METHOD OF DIPHTHERIA ANTIGENIC DIAGNOSTICUM PREPARING

METHOD OF DIPHTHERIA ANTIGENIC DIAGNOSTICUM PREPARING

medicine, microbiology. SUBSTANCE: invention relates to preparing diagnosticum by culturing diphtheria microorganisms on the solid nutrient medium followed by preparing a bacterial suspension with density 1000 OU/ml, its heating at +56-58 C for 40 min, treatment with formalin and merthiolate, washin...

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Hauptverfasser: MOSKALENKO E.P, SYLKA O.I, KHARSEEVA G.G
Format: Patent
Sprache:eng ; rus
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HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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creator MOSKALENKO E.P
SYLKA O.I
KHARSEEVA G.G
description medicine, microbiology. SUBSTANCE: invention relates to preparing diagnosticum by culturing diphtheria microorganisms on the solid nutrient medium followed by preparing a bacterial suspension with density 1000 OU/ml, its heating at +56-58 C for 40 min, treatment with formalin and merthiolate, washing out with 0.15 M sodium chloride solution, disintegration, isolation from suspension a diphtheria antigen by dialysis through a membrane with pores 40-60 nm, dilution of an antigen to concentration 250-400 mcg/ml by protein and its combination in volume 1 ml with 3 ml of 50% formalinized sheep erythrocytes washed out with 0.15 M sodium chloride solution, 5 ml of 20% solution of polyethylene glycol with molecular mass 6000 kDa and 4 drops of formalin. Then the prepared suspension is kept at temperature +22-24 C for 2 h and at +4-8 C for 24 h. Invention can be used for diagnosis of diphtheria. EFFECT: decreased time of diagnosticum preparing, enhanced effectiveness of diagnosticum. 1 tbl, 2 ex
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SUBSTANCE: invention relates to preparing diagnosticum by culturing diphtheria microorganisms on the solid nutrient medium followed by preparing a bacterial suspension with density 1000 OU/ml, its heating at +56-58 C for 40 min, treatment with formalin and merthiolate, washing out with 0.15 M sodium chloride solution, disintegration, isolation from suspension a diphtheria antigen by dialysis through a membrane with pores 40-60 nm, dilution of an antigen to concentration 250-400 mcg/ml by protein and its combination in volume 1 ml with 3 ml of 50% formalinized sheep erythrocytes washed out with 0.15 M sodium chloride solution, 5 ml of 20% solution of polyethylene glycol with molecular mass 6000 kDa and 4 drops of formalin. Then the prepared suspension is kept at temperature +22-24 C for 2 h and at +4-8 C for 24 h. Invention can be used for diagnosis of diphtheria. 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subjects HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
title METHOD OF DIPHTHERIA ANTIGENIC DIAGNOSTICUM PREPARING
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