METHOD OF INDUSTRIAL PRODUCING HUMAN RECOMBINANT GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR
FIELD: biotechnology. SUBSTANCE: recombinant strain of Escherichia coli Sg20050/p280 gM is cultured on standard LB-medium at 25-37 C. Culturing is carried out in the presence of ampicillin and tetracycline. The concentration of dissolved oxygen is 10-40% of saturation. Culturing can be performed at...
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| creator | NAUMOVA N.V PIKALOVA M.I KOLOKOL'TSOV A.A KHAJBULLINA I.I GILEVA I.P DOMANOVA Z.M |
| description | FIELD: biotechnology. SUBSTANCE: recombinant strain of Escherichia coli Sg20050/p280 gM is cultured on standard LB-medium at 25-37 C. Culturing is carried out in the presence of ampicillin and tetracycline. The concentration of dissolved oxygen is 10-40% of saturation. Culturing can be performed at 29 ± 1 C. Culturing can be performed for three stages at successive increase of nutrient medium volume from 50 ml to 100 l. Invention ensures to provide the maximal accumulation of glycoprotein of granulocyte- -macrophage colony-stimulatin factor in bacterium cells. EFFECT: improved method of the factor producing, increased yield of the end product. 3 cl, 3 tbl, 2 ex |
| format | Patent |
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SUBSTANCE: recombinant strain of Escherichia coli Sg20050/p280 gM is cultured on standard LB-medium at 25-37 C. Culturing is carried out in the presence of ampicillin and tetracycline. The concentration of dissolved oxygen is 10-40% of saturation. Culturing can be performed at 29 ± 1 C. Culturing can be performed for three stages at successive increase of nutrient medium volume from 50 ml to 100 l. Invention ensures to provide the maximal accumulation of glycoprotein of granulocyte- -macrophage colony-stimulatin factor in bacterium cells. EFFECT: improved method of the factor producing, increased yield of the end product. 3 cl, 3 tbl, 2 ex</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD OF INDUSTRIAL PRODUCING HUMAN RECOMBINANT GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR |
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