HIRUDINE DERIVATIVE AS THROMBIN INHIBITOR, RECOMBINANT DNA ENCODING HIRUDINE DERIVATIVE AND A METHOD OF ITS PREPARING
FIELD: biotechnology. SUBSTANCE: invention relates to preparing derivative of hirudine that acts as a thrombin inhibitor and exhibiting specific activity 10 000 AT U/mg, not less. Amino acid sequence of this derivative of hirudine is identified. Also, nucleotide sequence of recombinant DNA encoding...
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| creator | GERKHARD SHMID PAUL' KHABERMANN |
| description | FIELD: biotechnology. SUBSTANCE: invention relates to preparing derivative of hirudine that acts as a thrombin inhibitor and exhibiting specific activity 10 000 AT U/mg, not less. Amino acid sequence of this derivative of hirudine is identified. Also, nucleotide sequence of recombinant DNA encoding this derivative of hirudine is identified. Method of preparing hirudine derivative involves plasmid construction containing recombinant DNA and its using for transformation of a mutant-producer of Escherichia coli, culturing the transformed cells in medium and isolation of the end product. EFFECT: hirudine derivative shows specific activity as a thrombin inhibitor. 3 cl, 5 dwgc |
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SUBSTANCE: invention relates to preparing derivative of hirudine that acts as a thrombin inhibitor and exhibiting specific activity 10 000 AT U/mg, not less. Amino acid sequence of this derivative of hirudine is identified. Also, nucleotide sequence of recombinant DNA encoding this derivative of hirudine is identified. Method of preparing hirudine derivative involves plasmid construction containing recombinant DNA and its using for transformation of a mutant-producer of Escherichia coli, culturing the transformed cells in medium and isolation of the end product. 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SUBSTANCE: invention relates to preparing derivative of hirudine that acts as a thrombin inhibitor and exhibiting specific activity 10 000 AT U/mg, not less. Amino acid sequence of this derivative of hirudine is identified. Also, nucleotide sequence of recombinant DNA encoding this derivative of hirudine is identified. Method of preparing hirudine derivative involves plasmid construction containing recombinant DNA and its using for transformation of a mutant-producer of Escherichia coli, culturing the transformed cells in medium and isolation of the end product. 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SUBSTANCE: invention relates to preparing derivative of hirudine that acts as a thrombin inhibitor and exhibiting specific activity 10 000 AT U/mg, not less. Amino acid sequence of this derivative of hirudine is identified. Also, nucleotide sequence of recombinant DNA encoding this derivative of hirudine is identified. Method of preparing hirudine derivative involves plasmid construction containing recombinant DNA and its using for transformation of a mutant-producer of Escherichia coli, culturing the transformed cells in medium and isolation of the end product. EFFECT: hirudine derivative shows specific activity as a thrombin inhibitor. 3 cl, 5 dwgc</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | HIRUDINE DERIVATIVE AS THROMBIN INHIBITOR, RECOMBINANT DNA ENCODING HIRUDINE DERIVATIVE AND A METHOD OF ITS PREPARING |
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