PRODUCTION AND USE OF MODERN OVOTRANSFERRIN ()
The invention relates to a method for producing the ovotransferrin OTF-M specific for a target antigen, with applications in medicine. According to the invention, the method consists in the immunisation of the laying hens with an antigen consisting of a mixture of bacterial strains resistant to anti...
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Zusammenfassung: | The invention relates to a method for producing the ovotransferrin OTF-M specific for a target antigen, with applications in medicine. According to the invention, the method consists in the immunisation of the laying hens with an antigen consisting of a mixture of bacterial strains resistant to antibiotics, isolation of the white from the eggs produced by the immunised hens, followed by diluting it in a ratio of 1:1 with deionized water, adjusting the pH to 5, settling in two stages with ammonium sulphate and citric acid, followed by dialysis and lyophilisation.
Invenţia se referă la o metodă de producere a ovotransferinei specifice OTF-M pentru un antigen ţintă cu aplicaţii în medicină. Metoda conform invenţiei constă în imunizarea găinilor ouătoare cu un antigen format dintr-un amestec de tulpini bacteriene rezistente la antibiotice, izolarea albuşului din ouăle care provin de la găinile imunizate, diluarea acestuia în raport de 1:1 cu apă deionizată, ajustareaH la 5, precipitarea în două etape cu sulfat de amoniu şi acid citric, urmată de dializă şi liofilizare. |
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