A METHOD FOR ISOLATION OF NUCLEIC ACIDS AND A KIT THEREOF
The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes th...
Gespeichert in:
Hauptverfasser: | , , , , , |
---|---|
Format: | Patent |
Sprache: | eng ; por |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | |
container_start_page | |
container_title | |
container_volume | |
creator | MULAKKAPURATH NARAYANAN MANOJ PILLARISETTI VENKATA SUBBARAO PHANI KUMAR PULLELA CHANDRASEKHAR BHASKARAN NAIR MITCHELL PREETHAM PINTO SANTHOSH KUMAR GANDHAVALLA |
description | The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs. |
format | Patent |
fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_PT2521780TT</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>PT2521780TT</sourcerecordid><originalsourceid>FETCH-epo_espacenet_PT2521780TT3</originalsourceid><addsrcrecordid>eNrjZLB0VPB1DfHwd1Fw8w9S8Az293EM8fT3U_B3U_ALdfZx9XRWcHT2dAlWcPRzUXBU8PYMUQjxcA1y9XfjYWBNS8wpTuWF0twMCm6uIc4euqkF-fGpxQWJyal5qSXxASFGpkaG5hYGISHGRCgBAPaLKCQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>A METHOD FOR ISOLATION OF NUCLEIC ACIDS AND A KIT THEREOF</title><source>esp@cenet</source><creator>MULAKKAPURATH NARAYANAN MANOJ ; PILLARISETTI VENKATA SUBBARAO ; PHANI KUMAR PULLELA ; CHANDRASEKHAR BHASKARAN NAIR ; MITCHELL PREETHAM PINTO ; SANTHOSH KUMAR GANDHAVALLA</creator><creatorcontrib>MULAKKAPURATH NARAYANAN MANOJ ; PILLARISETTI VENKATA SUBBARAO ; PHANI KUMAR PULLELA ; CHANDRASEKHAR BHASKARAN NAIR ; MITCHELL PREETHAM PINTO ; SANTHOSH KUMAR GANDHAVALLA</creatorcontrib><description>The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.</description><language>eng ; por</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20171221&DB=EPODOC&CC=PT&NR=2521780T$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20171221&DB=EPODOC&CC=PT&NR=2521780T$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>MULAKKAPURATH NARAYANAN MANOJ</creatorcontrib><creatorcontrib>PILLARISETTI VENKATA SUBBARAO</creatorcontrib><creatorcontrib>PHANI KUMAR PULLELA</creatorcontrib><creatorcontrib>CHANDRASEKHAR BHASKARAN NAIR</creatorcontrib><creatorcontrib>MITCHELL PREETHAM PINTO</creatorcontrib><creatorcontrib>SANTHOSH KUMAR GANDHAVALLA</creatorcontrib><title>A METHOD FOR ISOLATION OF NUCLEIC ACIDS AND A KIT THEREOF</title><description>The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLB0VPB1DfHwd1Fw8w9S8Az293EM8fT3U_B3U_ALdfZx9XRWcHT2dAlWcPRzUXBU8PYMUQjxcA1y9XfjYWBNS8wpTuWF0twMCm6uIc4euqkF-fGpxQWJyal5qSXxASFGpkaG5hYGISHGRCgBAPaLKCQ</recordid><startdate>20171221</startdate><enddate>20171221</enddate><creator>MULAKKAPURATH NARAYANAN MANOJ</creator><creator>PILLARISETTI VENKATA SUBBARAO</creator><creator>PHANI KUMAR PULLELA</creator><creator>CHANDRASEKHAR BHASKARAN NAIR</creator><creator>MITCHELL PREETHAM PINTO</creator><creator>SANTHOSH KUMAR GANDHAVALLA</creator><scope>EVB</scope></search><sort><creationdate>20171221</creationdate><title>A METHOD FOR ISOLATION OF NUCLEIC ACIDS AND A KIT THEREOF</title><author>MULAKKAPURATH NARAYANAN MANOJ ; PILLARISETTI VENKATA SUBBARAO ; PHANI KUMAR PULLELA ; CHANDRASEKHAR BHASKARAN NAIR ; MITCHELL PREETHAM PINTO ; SANTHOSH KUMAR GANDHAVALLA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_PT2521780TT3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; por</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>MULAKKAPURATH NARAYANAN MANOJ</creatorcontrib><creatorcontrib>PILLARISETTI VENKATA SUBBARAO</creatorcontrib><creatorcontrib>PHANI KUMAR PULLELA</creatorcontrib><creatorcontrib>CHANDRASEKHAR BHASKARAN NAIR</creatorcontrib><creatorcontrib>MITCHELL PREETHAM PINTO</creatorcontrib><creatorcontrib>SANTHOSH KUMAR GANDHAVALLA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>MULAKKAPURATH NARAYANAN MANOJ</au><au>PILLARISETTI VENKATA SUBBARAO</au><au>PHANI KUMAR PULLELA</au><au>CHANDRASEKHAR BHASKARAN NAIR</au><au>MITCHELL PREETHAM PINTO</au><au>SANTHOSH KUMAR GANDHAVALLA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>A METHOD FOR ISOLATION OF NUCLEIC ACIDS AND A KIT THEREOF</title><date>2017-12-21</date><risdate>2017</risdate><abstract>The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.</abstract><oa>free_for_read</oa></addata></record> |
fulltext | fulltext_linktorsrc |
identifier | |
ispartof | |
issn | |
language | eng ; por |
recordid | cdi_epo_espacenet_PT2521780TT |
source | esp@cenet |
subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
title | A METHOD FOR ISOLATION OF NUCLEIC ACIDS AND A KIT THEREOF |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T14%3A34%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=MULAKKAPURATH%20NARAYANAN%20MANOJ&rft.date=2017-12-21&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EPT2521780TT%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |