ENHANCED MULTIPLEX FISH

Subject of the present invention is a combination of nucleic acid molecules capable of hybridising with a target nucleic acid sequence. In order to overcome problems with the reproducibility of FISH assays and to decrease assay time, hairpin probes are used in combination with helper probes annealin...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: WALTER FREIHERR VON STEIN, IAN PETER THRIPPLETON
Format: Patent
Sprache:eng ; por
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Beschreibung
Zusammenfassung:Subject of the present invention is a combination of nucleic acid molecules capable of hybridising with a target nucleic acid sequence. In order to overcome problems with the reproducibility of FISH assays and to decrease assay time, hairpin probes are used in combination with helper probes annealing adjacent to the target site of the hairpin probe.