OXIDIZED AVIDIN WITH HIGH RESIDENCY TIME IN THE TREATED TISSUES

OXIDIZED AVIDIN WITH HIGH RESIDENCY TIME IN THE TREATED TISSUES

The present invention describes chemically modified avidins that have higher permanence in treated tissues compared to wild type avidin. Avidin oxidation is performed by periodate incubation in the presence of the low affinity ligand HABA which, occupying the biotin binding sites, prevents protein d...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: RITA DE SANTIS, CARLO ANTONIO NUZZOLO
Format: Patent
Sprache:eng ; por
Schlagworte:
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator RITA DE SANTIS
CARLO ANTONIO NUZZOLO
description The present invention describes chemically modified avidins that have higher permanence in treated tissues compared to wild type avidin. Avidin oxidation is performed by periodate incubation in the presence of the low affinity ligand HABA which, occupying the biotin binding sites, prevents protein denaturation during the oxidation step. Periodate oxidation generates CHO groups from avidin mannose ring opening that, once injected, react with tissue NH2 residues to form stable Schiff's bases. The anchored avidins maintain the ability to bind biotinylated agents endowed of therapeutic activity, like radiolabeled biotins, stem cells and somatic cells, useful for brachytherapies like Intraoperative Avidination Radionuclide Therapy (IART®) or degenerative or genetic diseases.
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_PT2185203TT</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>PT2185203TT</sourcerecordid><originalsourceid>FETCH-epo_espacenet_PT2185203TT3</originalsourceid><addsrcrecordid>eNrjZLD3j_B08YxydVFwDAMy_BTCPUM8FDw83T0UglyDPV1c_ZwjFUI8fV0VgHIhHq4KIUGujiFA5SGewcGhrsE8DKxpiTnFqbxQmptBwc01xNlDN7UgPz61uCAxOTUvtSQ-IMTI0MLUyMA4JMSYCCUALiIqXw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>OXIDIZED AVIDIN WITH HIGH RESIDENCY TIME IN THE TREATED TISSUES</title><source>esp@cenet</source><creator>RITA DE SANTIS ; CARLO ANTONIO NUZZOLO</creator><creatorcontrib>RITA DE SANTIS ; CARLO ANTONIO NUZZOLO</creatorcontrib><description>The present invention describes chemically modified avidins that have higher permanence in treated tissues compared to wild type avidin. Avidin oxidation is performed by periodate incubation in the presence of the low affinity ligand HABA which, occupying the biotin binding sites, prevents protein denaturation during the oxidation step. Periodate oxidation generates CHO groups from avidin mannose ring opening that, once injected, react with tissue NH2 residues to form stable Schiff's bases. The anchored avidins maintain the ability to bind biotinylated agents endowed of therapeutic activity, like radiolabeled biotins, stem cells and somatic cells, useful for brachytherapies like Intraoperative Avidination Radionuclide Therapy (IART®) or degenerative or genetic diseases.</description><language>eng ; por</language><subject>HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20170602&amp;DB=EPODOC&amp;CC=PT&amp;NR=2185203T$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20170602&amp;DB=EPODOC&amp;CC=PT&amp;NR=2185203T$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>RITA DE SANTIS</creatorcontrib><creatorcontrib>CARLO ANTONIO NUZZOLO</creatorcontrib><title>OXIDIZED AVIDIN WITH HIGH RESIDENCY TIME IN THE TREATED TISSUES</title><description>The present invention describes chemically modified avidins that have higher permanence in treated tissues compared to wild type avidin. Avidin oxidation is performed by periodate incubation in the presence of the low affinity ligand HABA which, occupying the biotin binding sites, prevents protein denaturation during the oxidation step. Periodate oxidation generates CHO groups from avidin mannose ring opening that, once injected, react with tissue NH2 residues to form stable Schiff's bases. The anchored avidins maintain the ability to bind biotinylated agents endowed of therapeutic activity, like radiolabeled biotins, stem cells and somatic cells, useful for brachytherapies like Intraoperative Avidination Radionuclide Therapy (IART®) or degenerative or genetic diseases.</description><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLD3j_B08YxydVFwDAMy_BTCPUM8FDw83T0UglyDPV1c_ZwjFUI8fV0VgHIhHq4KIUGujiFA5SGewcGhrsE8DKxpiTnFqbxQmptBwc01xNlDN7UgPz61uCAxOTUvtSQ-IMTI0MLUyMA4JMSYCCUALiIqXw</recordid><startdate>20170602</startdate><enddate>20170602</enddate><creator>RITA DE SANTIS</creator><creator>CARLO ANTONIO NUZZOLO</creator><scope>EVB</scope></search><sort><creationdate>20170602</creationdate><title>OXIDIZED AVIDIN WITH HIGH RESIDENCY TIME IN THE TREATED TISSUES</title><author>RITA DE SANTIS ; CARLO ANTONIO NUZZOLO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_PT2185203TT3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; por</language><creationdate>2017</creationdate><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><toplevel>online_resources</toplevel><creatorcontrib>RITA DE SANTIS</creatorcontrib><creatorcontrib>CARLO ANTONIO NUZZOLO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>RITA DE SANTIS</au><au>CARLO ANTONIO NUZZOLO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>OXIDIZED AVIDIN WITH HIGH RESIDENCY TIME IN THE TREATED TISSUES</title><date>2017-06-02</date><risdate>2017</risdate><abstract>The present invention describes chemically modified avidins that have higher permanence in treated tissues compared to wild type avidin. Avidin oxidation is performed by periodate incubation in the presence of the low affinity ligand HABA which, occupying the biotin binding sites, prevents protein denaturation during the oxidation step. Periodate oxidation generates CHO groups from avidin mannose ring opening that, once injected, react with tissue NH2 residues to form stable Schiff's bases. The anchored avidins maintain the ability to bind biotinylated agents endowed of therapeutic activity, like radiolabeled biotins, stem cells and somatic cells, useful for brachytherapies like Intraoperative Avidination Radionuclide Therapy (IART®) or degenerative or genetic diseases.</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng ; por
recordid cdi_epo_espacenet_PT2185203TT
source esp@cenet
subjects HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
title OXIDIZED AVIDIN WITH HIGH RESIDENCY TIME IN THE TREATED TISSUES
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T10%3A16%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=RITA%20DE%20SANTIS&rft.date=2017-06-02&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EPT2185203TT%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true