METODO ENZIMATICO DE PRODUCAO DE 4-0-B-D-GALACTOPIRANOSIL-D-XILOSE, 4-0-B-D-XILOSE OBTIDA COM O REFERIDO METODO, COMPOSICOES QUE AS CONTEM E A SUA UTILIZACAO NA AVALIACAO DA LACTASE INTESTINAL

In the preparation of 4-O-beta -D-galactopyranosyl-D-xylose (I) by reacting D-xylose, a beta -galactopyranoside substrate and beta -D-galactosidase (BDG) in aqueous buffer, (I) is isolated (after deactivating or removing the BDG and removing the substrate aglycone) by adding celite, carrying out sol...

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Hauptverfasser: MANUEL MARTIN LOMAS, CANADA VICINAY, FRANCISCO, JAVIER, CORRALES MORALES, G, FDEZ-MAYORALAS ALVAREZ, A, ARAGON REYES, JUAN JOSE
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creator MANUEL MARTIN LOMAS
CANADA VICINAY, FRANCISCO, JAVIER
CORRALES MORALES, G
FDEZ-MAYORALAS ALVAREZ, A
ARAGON REYES, JUAN JOSE
description In the preparation of 4-O-beta -D-galactopyranosyl-D-xylose (I) by reacting D-xylose, a beta -galactopyranoside substrate and beta -D-galactosidase (BDG) in aqueous buffer, (I) is isolated (after deactivating or removing the BDG and removing the substrate aglycone) by adding celite, carrying out solid-liquid extraction and eluting from a column, and (I)-containing fractions are treated by crystallization. Preparation of 4-O-beta -D-galactopyranosyl-D-xylose (I) involves: (i) treating a mixture of 2-20 wt.% D-xylose, 0.5-5 wt.% of a beta -galactopyranoside (as substrate) and 75-97.5 wt.% aqueous buffer of pH 5.0-9.0 (as reaction medium) with 10-1000 U (per g of substrate) of beta -D-galactosidase (BDG); (ii) carrying out disaccharide-forming reaction at a temperature between the freezing point of the mixture and 45[deg]C for 2-48 hours; (iii) deactivating the BDG by freezing at -20 to -170[deg]C or by heating at 95-115[deg]C or removing the BDG from the mixture by ultrafiltration; (iv) removing the aglycone of the substrate by extraction or filtration, and (v) isolating the (I)-containing fractions. Step (v) is carried out by adding celite, carrying out solid-liquid extraction with a solvent and eluting from a column with a first eluant, or by directly adding activated carbon, filtering and eluting with a second solvent. The (I)-containing fractions are further treated by crystallization from a mixture of acetone and methanol (or water) in a ratio of 5-20:1. An independent claim is included for (I) obtained by the process.
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Preparation of 4-O-beta -D-galactopyranosyl-D-xylose (I) involves: (i) treating a mixture of 2-20 wt.% D-xylose, 0.5-5 wt.% of a beta -galactopyranoside (as substrate) and 75-97.5 wt.% aqueous buffer of pH 5.0-9.0 (as reaction medium) with 10-1000 U (per g of substrate) of beta -D-galactosidase (BDG); (ii) carrying out disaccharide-forming reaction at a temperature between the freezing point of the mixture and 45[deg]C for 2-48 hours; (iii) deactivating the BDG by freezing at -20 to -170[deg]C or by heating at 95-115[deg]C or removing the BDG from the mixture by ultrafiltration; (iv) removing the aglycone of the substrate by extraction or filtration, and (v) isolating the (I)-containing fractions. Step (v) is carried out by adding celite, carrying out solid-liquid extraction with a solvent and eluting from a column with a first eluant, or by directly adding activated carbon, filtering and eluting with a second solvent. 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Preparation of 4-O-beta -D-galactopyranosyl-D-xylose (I) involves: (i) treating a mixture of 2-20 wt.% D-xylose, 0.5-5 wt.% of a beta -galactopyranoside (as substrate) and 75-97.5 wt.% aqueous buffer of pH 5.0-9.0 (as reaction medium) with 10-1000 U (per g of substrate) of beta -D-galactosidase (BDG); (ii) carrying out disaccharide-forming reaction at a temperature between the freezing point of the mixture and 45[deg]C for 2-48 hours; (iii) deactivating the BDG by freezing at -20 to -170[deg]C or by heating at 95-115[deg]C or removing the BDG from the mixture by ultrafiltration; (iv) removing the aglycone of the substrate by extraction or filtration, and (v) isolating the (I)-containing fractions. Step (v) is carried out by adding celite, carrying out solid-liquid extraction with a solvent and eluting from a column with a first eluant, or by directly adding activated carbon, filtering and eluting with a second solvent. The (I)-containing fractions are further treated by crystallization from a mixture of acetone and methanol (or water) in a ratio of 5-20:1. An independent claim is included for (I) obtained by the process.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
DERIVATIVES THEREOF
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
HUMAN NECESSITIES
HYGIENE
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROCESSES USING MICROORGANISMS
SEPARATION
SPIRITS
SUGARS
TRANSPORTING
VINEGAR
WINE
title METODO ENZIMATICO DE PRODUCAO DE 4-0-B-D-GALACTOPIRANOSIL-D-XILOSE, 4-0-B-D-XILOSE OBTIDA COM O REFERIDO METODO, COMPOSICOES QUE AS CONTEM E A SUA UTILIZACAO NA AVALIACAO DA LACTASE INTESTINAL
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