High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris
Discloses a method of producing full length antibodies, comprising: (a) providing a growth culture comprising Pichia pastoris cells comprising genes that provide for the expression of said full length antibodies and growing said Pichia yeast in said growth culture for about 12-16 hours ("growth...
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| creator | QI PEI GARCIA-MARTINEZ LEON F MCNEILL PATRICIA DIANNE LATHAM JOHN A LESNICKI GARY L JANSON NICOLE |
| description | Discloses a method of producing full length antibodies, comprising: (a) providing a growth culture comprising Pichia pastoris cells comprising genes that provide for the expression of said full length antibodies and growing said Pichia yeast in said growth culture for about 12-16 hours ("growth phase"); (b) adding ethanol to said culture after said growth phase in a single bolus addition ("bolus") which bolus results in a concentration of ethanol in said culture of between 0.5% and 1.5% w/v, and (c) culturing said culture to produce said secreted full length antibodies , wherein said culturing comprises adding a feed comprising a fermentable carbon source to said culture. |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris |
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