High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris

High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris

Discloses a method of producing full length antibodies, comprising: (a) providing a growth culture comprising Pichia pastoris cells comprising genes that provide for the expression of said full length antibodies and growing said Pichia yeast in said growth culture for about 12-16 hours ("growth...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: QI PEI, GARCIA-MARTINEZ LEON F, MCNEILL PATRICIA DIANNE, LATHAM JOHN A, LESNICKI GARY L, JANSON NICOLE
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Discloses a method of producing full length antibodies, comprising: (a) providing a growth culture comprising Pichia pastoris cells comprising genes that provide for the expression of said full length antibodies and growing said Pichia yeast in said growth culture for about 12-16 hours ("growth phase"); (b) adding ethanol to said culture after said growth phase in a single bolus addition ("bolus") which bolus results in a concentration of ethanol in said culture of between 0.5% and 1.5% w/v, and (c) culturing said culture to produce said secreted full length antibodies , wherein said culturing comprises adding a feed comprising a fermentable carbon source to said culture.