Expression vector, methods for the production of heterologous gene products, and selection method for high-producing recombinant cells

Expression vector, methods for the production of heterologous gene products, and selection method for high-producing recombinant cells

Disclosed is an expression vector for eukaryotic cells comprising a gene that codes for a protein of interest in a functional bond with a hamster ubiquitin/S27a promoter, and a gene which codes for a fluorescent protein. Preferably, the expression vector also comprises an amplifiable selection marke...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: FIEDER, JUERGEN, OTTO, RALF, ENENKEL, BARBARA, GRAMMATIKOS, STEFANOS
Format: Patent
Sprache:eng
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator FIEDER, JUERGEN
OTTO, RALF
ENENKEL, BARBARA
GRAMMATIKOS, STEFANOS
description Disclosed is an expression vector for eukaryotic cells comprising a gene that codes for a protein of interest in a functional bond with a hamster ubiquitin/S27a promoter, and a gene which codes for a fluorescent protein. Preferably, the expression vector also comprises an amplifiable selection marker gene. Also disclosed are host cells, preferably mammal cells, which are transfected with the expression vector, methods for producing heterologous gene products, and a method for selecting high-producing cells.
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_NZ540950A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>NZ540950A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_NZ540950A3</originalsourceid><addsrcrecordid>eNqFzT0KwkAQBeA0FqJeQeYACQQ0hWWQiJWVlU1YN5Pdhc3OsjMRT-C5_YnYWr3ifbw3zx7NPSZkdhTghloo5TCgWOoYekogFiEm6kYtb0I9WBRM5MnQyGAw_HrOQYUOGD1OeNr5zFhnbDE5Fwwk1DRcXVBBQKP3vMxmvfKMq28usvWhOe-PBUZqkaPSryNpT5dqW-6qst78BU_xp0tH</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Expression vector, methods for the production of heterologous gene products, and selection method for high-producing recombinant cells</title><source>esp@cenet</source><creator>FIEDER, JUERGEN ; OTTO, RALF ; ENENKEL, BARBARA ; GRAMMATIKOS, STEFANOS</creator><creatorcontrib>FIEDER, JUERGEN ; OTTO, RALF ; ENENKEL, BARBARA ; GRAMMATIKOS, STEFANOS</creatorcontrib><description>Disclosed is an expression vector for eukaryotic cells comprising a gene that codes for a protein of interest in a functional bond with a hamster ubiquitin/S27a promoter, and a gene which codes for a fluorescent protein. Preferably, the expression vector also comprises an amplifiable selection marker gene. Also disclosed are host cells, preferably mammal cells, which are transfected with the expression vector, methods for producing heterologous gene products, and a method for selecting high-producing cells.</description><edition>7</edition><language>eng</language><creationdate>2008</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20080131&amp;DB=EPODOC&amp;CC=NZ&amp;NR=540950A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20080131&amp;DB=EPODOC&amp;CC=NZ&amp;NR=540950A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>FIEDER, JUERGEN</creatorcontrib><creatorcontrib>OTTO, RALF</creatorcontrib><creatorcontrib>ENENKEL, BARBARA</creatorcontrib><creatorcontrib>GRAMMATIKOS, STEFANOS</creatorcontrib><title>Expression vector, methods for the production of heterologous gene products, and selection method for high-producing recombinant cells</title><description>Disclosed is an expression vector for eukaryotic cells comprising a gene that codes for a protein of interest in a functional bond with a hamster ubiquitin/S27a promoter, and a gene which codes for a fluorescent protein. Preferably, the expression vector also comprises an amplifiable selection marker gene. Also disclosed are host cells, preferably mammal cells, which are transfected with the expression vector, methods for producing heterologous gene products, and a method for selecting high-producing cells.</description><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2008</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqFzT0KwkAQBeA0FqJeQeYACQQ0hWWQiJWVlU1YN5Pdhc3OsjMRT-C5_YnYWr3ifbw3zx7NPSZkdhTghloo5TCgWOoYekogFiEm6kYtb0I9WBRM5MnQyGAw_HrOQYUOGD1OeNr5zFhnbDE5Fwwk1DRcXVBBQKP3vMxmvfKMq28usvWhOe-PBUZqkaPSryNpT5dqW-6qst78BU_xp0tH</recordid><startdate>20080131</startdate><enddate>20080131</enddate><creator>FIEDER, JUERGEN</creator><creator>OTTO, RALF</creator><creator>ENENKEL, BARBARA</creator><creator>GRAMMATIKOS, STEFANOS</creator><scope>EVB</scope></search><sort><creationdate>20080131</creationdate><title>Expression vector, methods for the production of heterologous gene products, and selection method for high-producing recombinant cells</title><author>FIEDER, JUERGEN ; OTTO, RALF ; ENENKEL, BARBARA ; GRAMMATIKOS, STEFANOS</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_NZ540950A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2008</creationdate><toplevel>online_resources</toplevel><creatorcontrib>FIEDER, JUERGEN</creatorcontrib><creatorcontrib>OTTO, RALF</creatorcontrib><creatorcontrib>ENENKEL, BARBARA</creatorcontrib><creatorcontrib>GRAMMATIKOS, STEFANOS</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>FIEDER, JUERGEN</au><au>OTTO, RALF</au><au>ENENKEL, BARBARA</au><au>GRAMMATIKOS, STEFANOS</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Expression vector, methods for the production of heterologous gene products, and selection method for high-producing recombinant cells</title><date>2008-01-31</date><risdate>2008</risdate><abstract>Disclosed is an expression vector for eukaryotic cells comprising a gene that codes for a protein of interest in a functional bond with a hamster ubiquitin/S27a promoter, and a gene which codes for a fluorescent protein. Preferably, the expression vector also comprises an amplifiable selection marker gene. Also disclosed are host cells, preferably mammal cells, which are transfected with the expression vector, methods for producing heterologous gene products, and a method for selecting high-producing cells.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng
recordid cdi_epo_espacenet_NZ540950A
source esp@cenet
title Expression vector, methods for the production of heterologous gene products, and selection method for high-producing recombinant cells
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T04%3A53%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=FIEDER,%20JUERGEN&rft.date=2008-01-31&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ENZ540950A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true