RT-PCR of clonal neurospheres

A method is described for the preparation, characterization and utilization of temporally ordered panels of cDNA libraries from human microclones. Each microclone undergoing morphogenetic differentiation in vitro differs in discrete gene expression. The developmental stage of the microclone is deter...

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Hauptverfasser: STEINDLER, DENNIS A, KUKEKOV, VALERY G, SUSLOV, OLEG N
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KUKEKOV, VALERY G
SUSLOV, OLEG N
description A method is described for the preparation, characterization and utilization of temporally ordered panels of cDNA libraries from human microclones. Each microclone undergoing morphogenetic differentiation in vitro differs in discrete gene expression. The developmental stage of the microclone is determined and the gene expression patterns of the microclones temporally ordered and arranged into expression profiles and temporal spectra. Direct comparison of the cDNAs produced from different neural microclones allows identification of novel RNA transcripts.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title RT-PCR of clonal neurospheres
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