Fremgangsmåte for utförelse av en immunologisk bestemmelse av et antigen i en væskepröve samt reagens for bruk til dette

Fremgangsmåte for utförelse av en immunologisk bestemmelse av et antigen i en væskepröve samt reagens for bruk til dette

A method for obtaining an actual linear standard curve in a sandwich type of immunoassay where a first antibody bound to an insoluble support and a second unbound labelled antibody complex with the antigen contained in a test sample to form an insoluble antibody:antigen:labelled antibody complex whi...

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Hauptverfasser: LEE, SHIH-YUN, ROOD, FREDERICH WILLIAM JR, TUNG, KER-KONG, CRAGLE, LINDA KATHERINE
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator LEE, SHIH-YUN
ROOD, FREDERICH WILLIAM JR
TUNG, KER-KONG
CRAGLE, LINDA KATHERINE
description A method for obtaining an actual linear standard curve in a sandwich type of immunoassay where a first antibody bound to an insoluble support and a second unbound labelled antibody complex with the antigen contained in a test sample to form an insoluble antibody:antigen:labelled antibody complex which is then detected. Unbound unlabelled first antibody and/or unbound unlabelled second antibody are added to the reaction mixture to divert excess antigen away from the desired end-product complex, thus rendering the antigen of interest the rate-limiting factor in the overall immunoreaction. This results in a pseudo first-order reaction which produces an actual linear standard curve.
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Fremgangsmåte for utförelse av en immunologisk bestemmelse av et antigen i en væskepröve samt reagens for bruk til dette
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