A Preparation Method for Embedding n-3 Long Chain Polyunsaturated Fatty Acid Microcapsules

The invention discloses a preparation method of fish oil microcapsules based on emulsification and aggregation of pea protein isolate, which comprises the following steps: 81. Preparation of PPI solution and SBP solution: PPl and SBP are dissolved respectively 5 in phosphate buffer solution and stirred until fully dissolved to obtain PPl reserve solution and SBP reserve solution respectively; 82. Preparation of PPI stabilized oil-in-water emulsion of fish oil: PPl reserve solution was stirred and mixed with FO to obtain PPl oil-in- water emulsion of fish oil; 83. Preparation of FO-PPl-SBP complex condensates: PPl fish oil in water emulsion was mixed with SBP reserve solution, pH value was adjusted to 3.5- 10 4.5, and then standing for 24 hours to obtain FO-PPl-SBP complex condensates. 84. Preparation of fish oil microcapsules: the composite condensates of FO-PPl-SBP were sent into the micro spray dryer to form fish oil microcapsules through spray drying; By using FO- PPl-SBP composite condensate as the feed dispersion of fish oil microcapsules, this application greatly improves the powder yield, payload and encapsulation rate of fish oil 15 microcapsules, and improves the denaturation temperature and network thermal stability of fish oil microcapsules