LIGNIN DEGRADATION OF BIOMASS PRETREAMENT VIA OZONOLYSIS FOR ENHANCED GLUCOSE RECOVERY

The present invention provides a biomass pretreatments via ozonolysis to degrade lignin for enhanced glucose recovery. Accordingly, the biomass pretreament includes: a) an ozonolysis treatment at a reactor that is connected to an ozone generator and destructor, such that overnight-moistured biomass is being fed to the reactor, wherein ozone gas is being continuously passed through the reactor, and the oxygen is flashed into the reactor after the reaction is completed; b) sodium hydroxide (NaOH), wherein the sodium hydroxide (NaOH) is introduced into the reactor and such solution is then filtered and rinsed with distilled aquatic solution to remove the soluble compound before the treated biomass is being dried; and c) excess ozone is being destructed in the ozone destructor before being discharged to atmosphere; wherein lignin degradation is being determined by mixing the biomass with Sulfuric acid (H2SO4) with Potassium permanganate (KMnO4) and sugar monomers (i.e. glucose and xylose) of the biomass are being synthesized via a two-step acid hydrolysis. The most illustrative drawing: FIG. 2