METHOD FOR PURIFYING THE RABIES VIRUS
The subject matter of the invention is a method for purifying the rabies virus, comprising a single step of ion exchange chromatography, said step being a cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with...
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| creator | Pierre RIFFARD Eric CALVOSA Céline ROCCA Virginie FABRE |
| description | The subject matter of the invention is a method for purifying the rabies virus, comprising a single step of ion exchange chromatography, said step being a cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with a cation exchange chromatography substrate comprising a matrix of polymethacrylate onto which sulphoisobutyl groups have been grafted such that the rabies virus binds to this substrate, and b) the virus is eluted from the substrate thereof.
La presente invención se refiere a un método para purificar el virus de rabia, que comprende una sola etapa de cromatografía de intercambio iónico, la etapa que es una cromatografía de intercambio catiónico de acuerdo con la cual: a) el sobrenadante de un cultivo de células infectadas con este virus se lleva en contacto con un sustrato de cromatografía de intercambio catiónico que comprende una matriz de polimetacrilato sobre la cual se han injertado grupos de sulfoisobutilo tal que el virus de rabia se enlaza a este sustrato y b) el virus se eluye del sustrato mismo. |
| format | Patent |
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La presente invención se refiere a un método para purificar el virus de rabia, que comprende una sola etapa de cromatografía de intercambio iónico, la etapa que es una cromatografía de intercambio catiónico de acuerdo con la cual: a) el sobrenadante de un cultivo de células infectadas con este virus se lleva en contacto con un sustrato de cromatografía de intercambio catiónico que comprende una matriz de polimetacrilato sobre la cual se han injertado grupos de sulfoisobutilo tal que el virus de rabia se enlaza a este sustrato y b) el virus se eluye del sustrato mismo.</description><language>eng ; spa</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190920&DB=EPODOC&CC=MX&NR=368161B$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190920&DB=EPODOC&CC=MX&NR=368161B$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Pierre RIFFARD</creatorcontrib><creatorcontrib>Eric CALVOSA</creatorcontrib><creatorcontrib>Céline ROCCA</creatorcontrib><creatorcontrib>Virginie FABRE</creatorcontrib><title>METHOD FOR PURIFYING THE RABIES VIRUS</title><description>The subject matter of the invention is a method for purifying the rabies virus, comprising a single step of ion exchange chromatography, said step being a cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with a cation exchange chromatography substrate comprising a matrix of polymethacrylate onto which sulphoisobutyl groups have been grafted such that the rabies virus binds to this substrate, and b) the virus is eluted from the substrate thereof.
La presente invención se refiere a un método para purificar el virus de rabia, que comprende una sola etapa de cromatografía de intercambio iónico, la etapa que es una cromatografía de intercambio catiónico de acuerdo con la cual: a) el sobrenadante de un cultivo de células infectadas con este virus se lleva en contacto con un sustrato de cromatografía de intercambio catiónico que comprende una matriz de polimetacrilato sobre la cual se han injertado grupos de sulfoisobutilo tal que el virus de rabia se enlaza a este sustrato y b) el virus se eluye del sustrato mismo.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZFD1dQ3x8HdRcPMPUggIDfJ0i_T0c1cI8XBVCHJ08nQNVgjzDAoN5mFgTUvMKU7lhdLcDHJuriHOHrqpBfnxqcUFicmpeakl8b4RxmYWhmaGTsYEFQAA2roiNw</recordid><startdate>20190920</startdate><enddate>20190920</enddate><creator>Pierre RIFFARD</creator><creator>Eric CALVOSA</creator><creator>Céline ROCCA</creator><creator>Virginie FABRE</creator><scope>EVB</scope></search><sort><creationdate>20190920</creationdate><title>METHOD FOR PURIFYING THE RABIES VIRUS</title><author>Pierre RIFFARD ; Eric CALVOSA ; Céline ROCCA ; Virginie FABRE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_MX368161B3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; spa</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>Pierre RIFFARD</creatorcontrib><creatorcontrib>Eric CALVOSA</creatorcontrib><creatorcontrib>Céline ROCCA</creatorcontrib><creatorcontrib>Virginie FABRE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Pierre RIFFARD</au><au>Eric CALVOSA</au><au>Céline ROCCA</au><au>Virginie FABRE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR PURIFYING THE RABIES VIRUS</title><date>2019-09-20</date><risdate>2019</risdate><abstract>The subject matter of the invention is a method for purifying the rabies virus, comprising a single step of ion exchange chromatography, said step being a cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with a cation exchange chromatography substrate comprising a matrix of polymethacrylate onto which sulphoisobutyl groups have been grafted such that the rabies virus binds to this substrate, and b) the virus is eluted from the substrate thereof.
La presente invención se refiere a un método para purificar el virus de rabia, que comprende una sola etapa de cromatografía de intercambio iónico, la etapa que es una cromatografía de intercambio catiónico de acuerdo con la cual: a) el sobrenadante de un cultivo de células infectadas con este virus se lleva en contacto con un sustrato de cromatografía de intercambio catiónico que comprende una matriz de polimetacrilato sobre la cual se han injertado grupos de sulfoisobutilo tal que el virus de rabia se enlaza a este sustrato y b) el virus se eluye del sustrato mismo.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD FOR PURIFYING THE RABIES VIRUS |
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