EXPRESSION OF NITROGENASE POLYPEPTIDES IN PLANT CELLS

The present invention relates to methods and means for producing nitrogenase polypeptides in the mitochondria of plant cells. The present disclosure provides plant cells that express one or more MTP-Nif fusions and/or translational NifD-NifK and NifE-NifN fusions. The present disclosure also provide...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: OKADA Shoko, TILBROOK Kimberley Thelma, WARDEN Andrew Charles, WOOD Craig Christopher, TAYLOR Matthew Craig, ALLEN Robert Silas
Format: Patent
Sprache:eng ; spa
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator OKADA Shoko
TILBROOK Kimberley Thelma
WARDEN Andrew Charles
WOOD Craig Christopher
TAYLOR Matthew Craig
ALLEN Robert Silas
description The present invention relates to methods and means for producing nitrogenase polypeptides in the mitochondria of plant cells. The present disclosure provides plant cells that express one or more MTP-Nif fusions and/or translational NifD-NifK and NifE-NifN fusions. The present disclosure also provides nucleic acid constructs encoding these fusions as well as expression constructs for expression and targeting of the fusions to the mitochondria of plant cells. The present disclosure also provides transgenic plants comprising the plant cells of the invention and products obtained therefrom. La presente invención se refiere a métodos y medios para producir polipéptidos de nitrogenasa en las mitocondrias de células vegetales. La presente descripción proporciona células vegetales que expresan una o más fusiones MTP-Nif y/o fusiones NifD-NifK y NifE-NifN traslacionales. La presente descripción también proporciona constructos de ácidos nucleicos que codifican estas fusiones, así como también constructos de expresión para expresar y dirigir las fusiones a las mitocondrias de células vegetales. La presente descripción también proporciona plantas transgénicas que comprenden las células vegetales de la invención y productos obtenidos de esta.
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_MX2024000163A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>MX2024000163A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_MX2024000163A3</originalsourceid><addsrcrecordid>eNrjZDB1jQgIcg0O9vT3U_B3U_DzDAnyd3f1cwx2VQjw94kMcA0I8XRxDVbw9FMI8HH0C1FwdvXxCeZhYE1LzClO5YXS3AxKbq4hzh66qQX58anFBYnJqXmpJfG-EUYGRiYGBgaGZsaOxkQpAgBiKyhZ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>EXPRESSION OF NITROGENASE POLYPEPTIDES IN PLANT CELLS</title><source>esp@cenet</source><creator>OKADA Shoko ; TILBROOK Kimberley Thelma ; WARDEN Andrew Charles ; WOOD Craig Christopher ; TAYLOR Matthew Craig ; ALLEN Robert Silas</creator><creatorcontrib>OKADA Shoko ; TILBROOK Kimberley Thelma ; WARDEN Andrew Charles ; WOOD Craig Christopher ; TAYLOR Matthew Craig ; ALLEN Robert Silas</creatorcontrib><description>The present invention relates to methods and means for producing nitrogenase polypeptides in the mitochondria of plant cells. The present disclosure provides plant cells that express one or more MTP-Nif fusions and/or translational NifD-NifK and NifE-NifN fusions. The present disclosure also provides nucleic acid constructs encoding these fusions as well as expression constructs for expression and targeting of the fusions to the mitochondria of plant cells. The present disclosure also provides transgenic plants comprising the plant cells of the invention and products obtained therefrom. La presente invención se refiere a métodos y medios para producir polipéptidos de nitrogenasa en las mitocondrias de células vegetales. La presente descripción proporciona células vegetales que expresan una o más fusiones MTP-Nif y/o fusiones NifD-NifK y NifE-NifN traslacionales. La presente descripción también proporciona constructos de ácidos nucleicos que codifican estas fusiones, así como también constructos de expresión para expresar y dirigir las fusiones a las mitocondrias de células vegetales. La presente descripción también proporciona plantas transgénicas que comprenden las células vegetales de la invención y productos obtenidos de esta.</description><language>eng ; spa</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20240124&amp;DB=EPODOC&amp;CC=MX&amp;NR=2024000163A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25562,76317</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20240124&amp;DB=EPODOC&amp;CC=MX&amp;NR=2024000163A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>OKADA Shoko</creatorcontrib><creatorcontrib>TILBROOK Kimberley Thelma</creatorcontrib><creatorcontrib>WARDEN Andrew Charles</creatorcontrib><creatorcontrib>WOOD Craig Christopher</creatorcontrib><creatorcontrib>TAYLOR Matthew Craig</creatorcontrib><creatorcontrib>ALLEN Robert Silas</creatorcontrib><title>EXPRESSION OF NITROGENASE POLYPEPTIDES IN PLANT CELLS</title><description>The present invention relates to methods and means for producing nitrogenase polypeptides in the mitochondria of plant cells. The present disclosure provides plant cells that express one or more MTP-Nif fusions and/or translational NifD-NifK and NifE-NifN fusions. The present disclosure also provides nucleic acid constructs encoding these fusions as well as expression constructs for expression and targeting of the fusions to the mitochondria of plant cells. The present disclosure also provides transgenic plants comprising the plant cells of the invention and products obtained therefrom. La presente invención se refiere a métodos y medios para producir polipéptidos de nitrogenasa en las mitocondrias de células vegetales. La presente descripción proporciona células vegetales que expresan una o más fusiones MTP-Nif y/o fusiones NifD-NifK y NifE-NifN traslacionales. La presente descripción también proporciona constructos de ácidos nucleicos que codifican estas fusiones, así como también constructos de expresión para expresar y dirigir las fusiones a las mitocondrias de células vegetales. La presente descripción también proporciona plantas transgénicas que comprenden las células vegetales de la invención y productos obtenidos de esta.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDB1jQgIcg0O9vT3U_B3U_DzDAnyd3f1cwx2VQjw94kMcA0I8XRxDVbw9FMI8HH0C1FwdvXxCeZhYE1LzClO5YXS3AxKbq4hzh66qQX58anFBYnJqXmpJfG-EUYGRiYGBgaGZsaOxkQpAgBiKyhZ</recordid><startdate>20240124</startdate><enddate>20240124</enddate><creator>OKADA Shoko</creator><creator>TILBROOK Kimberley Thelma</creator><creator>WARDEN Andrew Charles</creator><creator>WOOD Craig Christopher</creator><creator>TAYLOR Matthew Craig</creator><creator>ALLEN Robert Silas</creator><scope>EVB</scope></search><sort><creationdate>20240124</creationdate><title>EXPRESSION OF NITROGENASE POLYPEPTIDES IN PLANT CELLS</title><author>OKADA Shoko ; TILBROOK Kimberley Thelma ; WARDEN Andrew Charles ; WOOD Craig Christopher ; TAYLOR Matthew Craig ; ALLEN Robert Silas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_MX2024000163A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; spa</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>OKADA Shoko</creatorcontrib><creatorcontrib>TILBROOK Kimberley Thelma</creatorcontrib><creatorcontrib>WARDEN Andrew Charles</creatorcontrib><creatorcontrib>WOOD Craig Christopher</creatorcontrib><creatorcontrib>TAYLOR Matthew Craig</creatorcontrib><creatorcontrib>ALLEN Robert Silas</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>OKADA Shoko</au><au>TILBROOK Kimberley Thelma</au><au>WARDEN Andrew Charles</au><au>WOOD Craig Christopher</au><au>TAYLOR Matthew Craig</au><au>ALLEN Robert Silas</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>EXPRESSION OF NITROGENASE POLYPEPTIDES IN PLANT CELLS</title><date>2024-01-24</date><risdate>2024</risdate><abstract>The present invention relates to methods and means for producing nitrogenase polypeptides in the mitochondria of plant cells. The present disclosure provides plant cells that express one or more MTP-Nif fusions and/or translational NifD-NifK and NifE-NifN fusions. The present disclosure also provides nucleic acid constructs encoding these fusions as well as expression constructs for expression and targeting of the fusions to the mitochondria of plant cells. The present disclosure also provides transgenic plants comprising the plant cells of the invention and products obtained therefrom. La presente invención se refiere a métodos y medios para producir polipéptidos de nitrogenasa en las mitocondrias de células vegetales. La presente descripción proporciona células vegetales que expresan una o más fusiones MTP-Nif y/o fusiones NifD-NifK y NifE-NifN traslacionales. La presente descripción también proporciona constructos de ácidos nucleicos que codifican estas fusiones, así como también constructos de expresión para expresar y dirigir las fusiones a las mitocondrias de células vegetales. La presente descripción también proporciona plantas transgénicas que comprenden las células vegetales de la invención y productos obtenidos de esta.</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng ; spa
recordid cdi_epo_espacenet_MX2024000163A
source esp@cenet
subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title EXPRESSION OF NITROGENASE POLYPEPTIDES IN PLANT CELLS
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T19%3A35%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=OKADA%20Shoko&rft.date=2024-01-24&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EMX2024000163A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true