USE OF CAS9 PROTEIN FROM THE BACTERIUM PASTEURELLA PNEUMOTROPICA

The present invention describes a novel bacterial nuclease of a CRISPR-Cas9 system from the bacterium P. pneumotropica, as well as the use of said nuclease for creating strictly specific two-strand cuts in a DNA molecule. Said nuclease has unusual properties and can be used for changing the sequence...

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Bibliographische Detailangaben
Hauptverfasser:	VASILIEVA Aleksandra Andreevna, MUSHAROVA Olga Sergeevna, ABRAMOVA Marina Viktorovna, SEVERINOV Konstantin Viktorovich, ARTAMONOVA Daria Nikolaevna, FEDOROVA Iana Vitalievna, ARTAMONOVA Tatiana Olegovna, SELKOVA Polina Anatolevna, SHMAKOV Sergey Anatolievich, POBEGALOV George Evgenevich, GORYANIN Ignaty Igorevich, KHODORKOVSKII Mikhail Alekseevich, ANDREEVA Julia Valerevna, ZYUBKO Tatiana Igorevna, ARSENIEV Anatoliy Nikolaevich
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention describes a novel bacterial nuclease of a CRISPR-Cas9 system from the bacterium P. pneumotropica, as well as the use of said nuclease for creating strictly specific two-strand cuts in a DNA molecule. Said nuclease has unusual properties and can be used for changing the sequence of genomic DNA in a cell of a unicellular or multicellular organism. The invention thus increases the universality of accessible CRISPR-Cas9 systems, making it possible to use different Cas9 nuclease variants to cut genomic or plasmid DNA in various organisms, in a large number of specific sites and/or under different conditions. La presente invención describe una novedosa nucleasa bacteriana del sistema CRISPR-Cas9 de la bacteria P. pneumotropica, así como también su uso para producir rupturas de cadena doble estrictamente específicas en una molécula de ADN. Esta nucleasa tiene propiedades inusuales y se puede utilizar como herramienta de modificación de la secuencia de ADN genómico en la célula de un organismo unicelular o multicelular. Así, aumenta la versatilidad de los sistemas CRISPR-Cas9 disponibles, hecho que permite el uso de distintas variantes de nucleasas Cas9 para el corte de ADN genómico o plásmido de distintos organismos en un número mayor de sitios específicos y/o en condiciones específicas.