OPTIMIZED HOST/VECTOR SYSTEM FOR PRODUCING PROTECTIVE MONO- AND MULTIVALENT SUBUNIT VACCINES ON THE BASIS OF THE YEAST KLUYVEROMYCES LACTIS
The invention relates to recombinant Kluyveromyces lactis (K. lactis) yeasts which are capable of the highly efficient expression of one or more foreign proteins and are suitable for use as a vaccine for generating a protective immune response against pathogens. The invention provides in particular...
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| creator | BEHRENS Sven- Erik HÜHRLIMANN Hans Caspar GEBAUER Mandy BREUNIG Karin BEHRENS Martina |
| description | The invention relates to recombinant Kluyveromyces lactis (K. lactis) yeasts which are capable of the highly efficient expression of one or more foreign proteins and are suitable for use as a vaccine for generating a protective immune response against pathogens. The invention provides in particular K. lactis strains for the targeted cloning of foreign antigen-coding nucleic acids into the yeast genome of the K. lactis strain, which is characterized in that the K. lactis strain has integrated expression cassettes for foreign antigens as an alternative or in addition to the KILAC4 locus on the KIURA3-20 locus (KLLA0E22771g) and/or on the KIMET5-1 locus (KLLA0B03938g). The invention further relates to integrative expression vectors and to methods for producing the K. lactis strains of the invention as well as to the use thereof as vaccines.
La invención se refiere a levaduras de Kluyveromyces lactis recombinantes (K. lactis), las cuales son capaces de presentar una expresión altamente eficiente de una o más proteínas extrañas y son adecuadas para usarse como una vacuna para generar una respuesta inmunitaria protectora contra los patógenos. La invención proporciona en particular las cepas de K. lactis para la clonación dirigida de los ácidos nucleicos que codifican el antígeno extraño en el genoma de la levadura de la cepa de K. lactis, la cual se caracteriza porque la cepa de K. lactis tiene casetes de expresión integrados para los antígenos extraños como una alternativa o además del locus KILAC4 o el locus KIURA3-20 (KLLA0E2277 lg) y/o en el locus KIMET5-1 (KLLA0B03938g). La invención se refiere además a los vectores de expresión de integración y a los métodos para la producción de las cepas de K. lactis de la invención así como al uso de las mismas como vacunas. |
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La invención se refiere a levaduras de Kluyveromyces lactis recombinantes (K. lactis), las cuales son capaces de presentar una expresión altamente eficiente de una o más proteínas extrañas y son adecuadas para usarse como una vacuna para generar una respuesta inmunitaria protectora contra los patógenos. La invención proporciona en particular las cepas de K. lactis para la clonación dirigida de los ácidos nucleicos que codifican el antígeno extraño en el genoma de la levadura de la cepa de K. lactis, la cual se caracteriza porque la cepa de K. lactis tiene casetes de expresión integrados para los antígenos extraños como una alternativa o además del locus KILAC4 o el locus KIURA3-20 (KLLA0E2277 lg) y/o en el locus KIMET5-1 (KLLA0B03938g). La invención se refiere además a los vectores de expresión de integración y a los métodos para la producción de las cepas de K. lactis de la invención así como al uso de las mismas como vacunas.</description><language>eng ; spa</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201106&DB=EPODOC&CC=MX&NR=2020006846A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201106&DB=EPODOC&CC=MX&NR=2020006846A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>BEHRENS Sven- Erik</creatorcontrib><creatorcontrib>HÜHRLIMANN Hans Caspar</creatorcontrib><creatorcontrib>GEBAUER Mandy</creatorcontrib><creatorcontrib>BREUNIG Karin</creatorcontrib><creatorcontrib>BEHRENS Martina</creatorcontrib><title>OPTIMIZED HOST/VECTOR SYSTEM FOR PRODUCING PROTECTIVE MONO- AND MULTIVALENT SUBUNIT VACCINES ON THE BASIS OF THE YEAST KLUYVEROMYCES LACTIS</title><description>The invention relates to recombinant Kluyveromyces lactis (K. lactis) yeasts which are capable of the highly efficient expression of one or more foreign proteins and are suitable for use as a vaccine for generating a protective immune response against pathogens. The invention provides in particular K. lactis strains for the targeted cloning of foreign antigen-coding nucleic acids into the yeast genome of the K. lactis strain, which is characterized in that the K. lactis strain has integrated expression cassettes for foreign antigens as an alternative or in addition to the KILAC4 locus on the KIURA3-20 locus (KLLA0E22771g) and/or on the KIMET5-1 locus (KLLA0B03938g). The invention further relates to integrative expression vectors and to methods for producing the K. lactis strains of the invention as well as to the use thereof as vaccines.
La invención se refiere a levaduras de Kluyveromyces lactis recombinantes (K. lactis), las cuales son capaces de presentar una expresión altamente eficiente de una o más proteínas extrañas y son adecuadas para usarse como una vacuna para generar una respuesta inmunitaria protectora contra los patógenos. La invención proporciona en particular las cepas de K. lactis para la clonación dirigida de los ácidos nucleicos que codifican el antígeno extraño en el genoma de la levadura de la cepa de K. lactis, la cual se caracteriza porque la cepa de K. lactis tiene casetes de expresión integrados para los antígenos extraños como una alternativa o además del locus KILAC4 o el locus KIURA3-20 (KLLA0E2277 lg) y/o en el locus KIMET5-1 (KLLA0B03938g). La invención se refiere además a los vectores de expresión de integración y a los métodos para la producción de las cepas de K. lactis de la invención así como al uso de las mismas como vacunas.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNTD0OgjAUZnEw6h1e3IkNGuJaykMaaUvoKxEXQkydjJLgLby01XgAp-__m0cvU5NU8ow5lMbSpkVBpgHbWUIFRaB1Y3InpD58GIVYtgjKaBMD1zkoVwWHV6gJrMuclgQtF2GAFowGKhEybmUQxVd0yC3BsXJdi41RnQi9iodbu4xm1-E2-dUPF9G6QBJl7MdH76dxuPi7f_bqlLCEMZbudynf_lV6Awt5QFc</recordid><startdate>20201106</startdate><enddate>20201106</enddate><creator>BEHRENS Sven- Erik</creator><creator>HÜHRLIMANN Hans Caspar</creator><creator>GEBAUER Mandy</creator><creator>BREUNIG Karin</creator><creator>BEHRENS Martina</creator><scope>EVB</scope></search><sort><creationdate>20201106</creationdate><title>OPTIMIZED HOST/VECTOR SYSTEM FOR PRODUCING PROTECTIVE MONO- AND MULTIVALENT SUBUNIT VACCINES ON THE BASIS OF THE YEAST KLUYVEROMYCES LACTIS</title><author>BEHRENS Sven- Erik ; HÜHRLIMANN Hans Caspar ; GEBAUER Mandy ; BREUNIG Karin ; BEHRENS Martina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_MX2020006846A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; spa</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>BEHRENS Sven- Erik</creatorcontrib><creatorcontrib>HÜHRLIMANN Hans Caspar</creatorcontrib><creatorcontrib>GEBAUER Mandy</creatorcontrib><creatorcontrib>BREUNIG Karin</creatorcontrib><creatorcontrib>BEHRENS Martina</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>BEHRENS Sven- Erik</au><au>HÜHRLIMANN Hans Caspar</au><au>GEBAUER Mandy</au><au>BREUNIG Karin</au><au>BEHRENS Martina</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>OPTIMIZED HOST/VECTOR SYSTEM FOR PRODUCING PROTECTIVE MONO- AND MULTIVALENT SUBUNIT VACCINES ON THE BASIS OF THE YEAST KLUYVEROMYCES LACTIS</title><date>2020-11-06</date><risdate>2020</risdate><abstract>The invention relates to recombinant Kluyveromyces lactis (K. lactis) yeasts which are capable of the highly efficient expression of one or more foreign proteins and are suitable for use as a vaccine for generating a protective immune response against pathogens. The invention provides in particular K. lactis strains for the targeted cloning of foreign antigen-coding nucleic acids into the yeast genome of the K. lactis strain, which is characterized in that the K. lactis strain has integrated expression cassettes for foreign antigens as an alternative or in addition to the KILAC4 locus on the KIURA3-20 locus (KLLA0E22771g) and/or on the KIMET5-1 locus (KLLA0B03938g). The invention further relates to integrative expression vectors and to methods for producing the K. lactis strains of the invention as well as to the use thereof as vaccines.
La invención se refiere a levaduras de Kluyveromyces lactis recombinantes (K. lactis), las cuales son capaces de presentar una expresión altamente eficiente de una o más proteínas extrañas y son adecuadas para usarse como una vacuna para generar una respuesta inmunitaria protectora contra los patógenos. La invención proporciona en particular las cepas de K. lactis para la clonación dirigida de los ácidos nucleicos que codifican el antígeno extraño en el genoma de la levadura de la cepa de K. lactis, la cual se caracteriza porque la cepa de K. lactis tiene casetes de expresión integrados para los antígenos extraños como una alternativa o además del locus KILAC4 o el locus KIURA3-20 (KLLA0E2277 lg) y/o en el locus KIMET5-1 (KLLA0B03938g). La invención se refiere además a los vectores de expresión de integración y a los métodos para la producción de las cepas de K. lactis de la invención así como al uso de las mismas como vacunas.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | OPTIMIZED HOST/VECTOR SYSTEM FOR PRODUCING PROTECTIVE MONO- AND MULTIVALENT SUBUNIT VACCINES ON THE BASIS OF THE YEAST KLUYVEROMYCES LACTIS |
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