THERMOSTABLE BIOCATALYST COMBINATION FOR NUCLEOSIDE SYNTHESIS
The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more c...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng ; spa |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | RAFAEL MONTILLA AREVALO CRISTINA LOPEZ GOMEZ MARTA PASCUAL GILABERT CARLOS ESTEVEZ COMPANY VICTOR MANUEL DERONCELE THOMAS JOSEP CASTELLS BOLIART |
| description | The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PNPase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.
La presente invención se refiere a un vector de expresión recombinante que comprende: a) la secuencia que codifica una purina nucleósido fosforilasa (PNPasa, E.C. 2.4.2.1), b) la secuencia que codifica una uridina fosforilasa (UPasa, E.C. 2.4.2.3), c) o ambas; cada una de las secuencias unida operativamente con una o más secuencias de control que dirigen la producción de tales fosforilasas en un huésped de expresión adecuado; originándose tales secuencias de la clase Archaea Thermoprotei, caracterizado porque la PNPasa es de Sulfolobus solfataricus (SEQ ID NO. 7) y la UPasa es de Aeropyrum pernix (SEQ ID NO. 8). Además, la presente invención se refiere a un método de transglicosilación entre un nucleósido donante de azúcar y una base aceptora en presencia de iones fosfato, caracterizado porque tal método comprende el uso de una uridina fosforilasa (UPasa) de Aeropyrum pernix (NC_000854.2), una purina nucleósido fosforilasa (PNPasa) de Sulfolobus solfataricus (NC_002754.1), o una combinación de las mismas. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_MX2012007487A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>MX2012007487A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_MX2012007487A3</originalsourceid><addsrcrecordid>eNrjZLAN8XAN8vUPDnF08nFVcPL0d3YMcfSJDA5RcPb3dfL0cwzx9PdTcPMPUvALdfZx9Q_2dHFVCI70A2oL9gzmYWBNS8wpTuWF0twMSm6uIc4euqkF-fGpxQWJyal5qSXxvhFGBoZGBgbmJhbmjsZEKQIAw3Mq5A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>THERMOSTABLE BIOCATALYST COMBINATION FOR NUCLEOSIDE SYNTHESIS</title><source>esp@cenet</source><creator>RAFAEL MONTILLA AREVALO ; CRISTINA LOPEZ GOMEZ ; MARTA PASCUAL GILABERT ; CARLOS ESTEVEZ COMPANY ; VICTOR MANUEL DERONCELE THOMAS ; JOSEP CASTELLS BOLIART</creator><creatorcontrib>RAFAEL MONTILLA AREVALO ; CRISTINA LOPEZ GOMEZ ; MARTA PASCUAL GILABERT ; CARLOS ESTEVEZ COMPANY ; VICTOR MANUEL DERONCELE THOMAS ; JOSEP CASTELLS BOLIART</creatorcontrib><description>The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PNPase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.
La presente invención se refiere a un vector de expresión recombinante que comprende: a) la secuencia que codifica una purina nucleósido fosforilasa (PNPasa, E.C. 2.4.2.1), b) la secuencia que codifica una uridina fosforilasa (UPasa, E.C. 2.4.2.3), c) o ambas; cada una de las secuencias unida operativamente con una o más secuencias de control que dirigen la producción de tales fosforilasas en un huésped de expresión adecuado; originándose tales secuencias de la clase Archaea Thermoprotei, caracterizado porque la PNPasa es de Sulfolobus solfataricus (SEQ ID NO. 7) y la UPasa es de Aeropyrum pernix (SEQ ID NO. 8). Además, la presente invención se refiere a un método de transglicosilación entre un nucleósido donante de azúcar y una base aceptora en presencia de iones fosfato, caracterizado porque tal método comprende el uso de una uridina fosforilasa (UPasa) de Aeropyrum pernix (NC_000854.2), una purina nucleósido fosforilasa (PNPasa) de Sulfolobus solfataricus (NC_002754.1), o una combinación de las mismas.</description><language>eng ; spa</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20121106&DB=EPODOC&CC=MX&NR=2012007487A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76418</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20121106&DB=EPODOC&CC=MX&NR=2012007487A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>RAFAEL MONTILLA AREVALO</creatorcontrib><creatorcontrib>CRISTINA LOPEZ GOMEZ</creatorcontrib><creatorcontrib>MARTA PASCUAL GILABERT</creatorcontrib><creatorcontrib>CARLOS ESTEVEZ COMPANY</creatorcontrib><creatorcontrib>VICTOR MANUEL DERONCELE THOMAS</creatorcontrib><creatorcontrib>JOSEP CASTELLS BOLIART</creatorcontrib><title>THERMOSTABLE BIOCATALYST COMBINATION FOR NUCLEOSIDE SYNTHESIS</title><description>The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PNPase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.
La presente invención se refiere a un vector de expresión recombinante que comprende: a) la secuencia que codifica una purina nucleósido fosforilasa (PNPasa, E.C. 2.4.2.1), b) la secuencia que codifica una uridina fosforilasa (UPasa, E.C. 2.4.2.3), c) o ambas; cada una de las secuencias unida operativamente con una o más secuencias de control que dirigen la producción de tales fosforilasas en un huésped de expresión adecuado; originándose tales secuencias de la clase Archaea Thermoprotei, caracterizado porque la PNPasa es de Sulfolobus solfataricus (SEQ ID NO. 7) y la UPasa es de Aeropyrum pernix (SEQ ID NO. 8). Además, la presente invención se refiere a un método de transglicosilación entre un nucleósido donante de azúcar y una base aceptora en presencia de iones fosfato, caracterizado porque tal método comprende el uso de una uridina fosforilasa (UPasa) de Aeropyrum pernix (NC_000854.2), una purina nucleósido fosforilasa (PNPasa) de Sulfolobus solfataricus (NC_002754.1), o una combinación de las mismas.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2012</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLAN8XAN8vUPDnF08nFVcPL0d3YMcfSJDA5RcPb3dfL0cwzx9PdTcPMPUvALdfZx9Q_2dHFVCI70A2oL9gzmYWBNS8wpTuWF0twMSm6uIc4euqkF-fGpxQWJyal5qSXxvhFGBoZGBgbmJhbmjsZEKQIAw3Mq5A</recordid><startdate>20121106</startdate><enddate>20121106</enddate><creator>RAFAEL MONTILLA AREVALO</creator><creator>CRISTINA LOPEZ GOMEZ</creator><creator>MARTA PASCUAL GILABERT</creator><creator>CARLOS ESTEVEZ COMPANY</creator><creator>VICTOR MANUEL DERONCELE THOMAS</creator><creator>JOSEP CASTELLS BOLIART</creator><scope>EVB</scope></search><sort><creationdate>20121106</creationdate><title>THERMOSTABLE BIOCATALYST COMBINATION FOR NUCLEOSIDE SYNTHESIS</title><author>RAFAEL MONTILLA AREVALO ; CRISTINA LOPEZ GOMEZ ; MARTA PASCUAL GILABERT ; CARLOS ESTEVEZ COMPANY ; VICTOR MANUEL DERONCELE THOMAS ; JOSEP CASTELLS BOLIART</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_MX2012007487A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; spa</language><creationdate>2012</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>RAFAEL MONTILLA AREVALO</creatorcontrib><creatorcontrib>CRISTINA LOPEZ GOMEZ</creatorcontrib><creatorcontrib>MARTA PASCUAL GILABERT</creatorcontrib><creatorcontrib>CARLOS ESTEVEZ COMPANY</creatorcontrib><creatorcontrib>VICTOR MANUEL DERONCELE THOMAS</creatorcontrib><creatorcontrib>JOSEP CASTELLS BOLIART</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>RAFAEL MONTILLA AREVALO</au><au>CRISTINA LOPEZ GOMEZ</au><au>MARTA PASCUAL GILABERT</au><au>CARLOS ESTEVEZ COMPANY</au><au>VICTOR MANUEL DERONCELE THOMAS</au><au>JOSEP CASTELLS BOLIART</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>THERMOSTABLE BIOCATALYST COMBINATION FOR NUCLEOSIDE SYNTHESIS</title><date>2012-11-06</date><risdate>2012</risdate><abstract>The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PNPase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.
La presente invención se refiere a un vector de expresión recombinante que comprende: a) la secuencia que codifica una purina nucleósido fosforilasa (PNPasa, E.C. 2.4.2.1), b) la secuencia que codifica una uridina fosforilasa (UPasa, E.C. 2.4.2.3), c) o ambas; cada una de las secuencias unida operativamente con una o más secuencias de control que dirigen la producción de tales fosforilasas en un huésped de expresión adecuado; originándose tales secuencias de la clase Archaea Thermoprotei, caracterizado porque la PNPasa es de Sulfolobus solfataricus (SEQ ID NO. 7) y la UPasa es de Aeropyrum pernix (SEQ ID NO. 8). Además, la presente invención se refiere a un método de transglicosilación entre un nucleósido donante de azúcar y una base aceptora en presencia de iones fosfato, caracterizado porque tal método comprende el uso de una uridina fosforilasa (UPasa) de Aeropyrum pernix (NC_000854.2), una purina nucleósido fosforilasa (PNPasa) de Sulfolobus solfataricus (NC_002754.1), o una combinación de las mismas.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng ; spa |
| recordid | cdi_epo_espacenet_MX2012007487A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | THERMOSTABLE BIOCATALYST COMBINATION FOR NUCLEOSIDE SYNTHESIS |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T17%3A00%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=RAFAEL%20MONTILLA%20AREVALO&rft.date=2012-11-06&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EMX2012007487A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |