Process for cultivation of microalga Porphyridium cruentum CNMN-AR-01

The invention refers to biotechnology, namely to a process for cultivation of microalga Porphyridium cruentum in order to obtain the biomass with high lipid content. The process for cultivation of microalga Porphyridium cruentum CNMN-AR-01 comprises cultivation on a nutrient medium containing, g/L:...

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Hauptverfasser:	CHIRIAC Tatiana, VALUŢA Ana, RUDIC Valeriu, MISCU Vera, DJUR Svetlana, IAŢCO Iulia, DUMBRĂVEANU Veronica, TAŞCA Ion, ROTARI Mihaela, RUDI Ludmila, CEPOI Liliana
Format:	Patent
Sprache:	eng ; rum ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSESC01D OR C01F CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INORGANIC CHEMISTRY MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NANOTECHNOLOGY PERFORMING OPERATIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention refers to biotechnology, namely to a process for cultivation of microalga Porphyridium cruentum in order to obtain the biomass with high lipid content. The process for cultivation of microalga Porphyridium cruentum CNMN-AR-01 comprises cultivation on a nutrient medium containing, g/L: KCl 16.04; NaCl 12.52; KNO3 1.24; MgSO4·7H2O 2.5; CaCl2 0.118; K2HPO4·3H2O 0.5; KI 0.05; KBr 0.05; 1 mL/L solution containing, mg/L: H3BO3 2.86; MnCl2·4H2O 1.81; CuSO4·5H2O 0.08; MoO3 0.015, FeEDTA 0.5 mL, Au nanoparticles of 10 nm stabilized in citrate 0.023-0.027nM and disstiled water the rest, at a temperature of 25-28°C, pH 6.8-7.2, constant lighting of 50-57 µM photons/m2/s, for 14 days. The result of the invention consists in increasing the biosynthesis of lipids and their accumulation in the biomass of tmicroalga Porphyridium cruentum. Invenţia se referă la biotehnologie, şi anume la un procedeu de cultivare a microalgei Porphyridium cruentum în scopul obţinerii de biomasă cu conţinut sporit de lipide.Procedeul de cultivare a microalgei Porphyridium cruentum CNMN-AR-01include cultivarea pe un mediu nutritiv ce conţine, g/L: KCl 16,04; NaCl 12,52; KNO3 1,24; MgSO4·7H2O 2,5; CaCl2 0,118; K2HPO4·3H2O 0,5; KI 0,05; KBr 0,05; 1 mL/L soluţie ce conţine, mg/L: H3BO3 2,86; MnCl2·4H2O 1,81; CuSO4·5H2O 0,08; MoO3 0,015; FeEDTA 0,5 mL; nanoparticule de Au de 10 nm stabilizate în citrat 0,23-0,27 nM şi apă distilată restul, la temperatura de 25-28°C, pH 6,8-7,2, iluminarea continuă de 50-57 µM fotoni/m2.s, timp de 14 zile.Rezultatul invenţiei constă în sporirea biosintezei lipidelor şi acumulării lor în biomasa microalgei Porphyridium cruentum. Изобретение относится к биотехнологии, а именно к способу культивирования микроводоросли Porphyridium cruentum с целью получения биомассы с высоким содержанием липидов. Способ культивирования микроводоросли Porphyridium cruentum CNMN-AR-01 включает культивирование на питательной среде, содержащей, г/л: KCl 16,04; NaCl 12,52; KNO3 1,24; MgSO4·7H2O 2,5; CaCl2 0,118; K2HPO4·3H2O 0,5; KI 0,05; KBr 0,05; 1 мл/л раствора, содержащего, мг/л: H3BO3 2,86; MnCl2·4H2O 1,81; CuSO4·5H2O 0,08; MoO3 0,015; FeEDTA 0,5 мл; наночастицы Au 10 нм стабилизированные в цитрате 0,023-0,027 нМ и дистиллированную воду остальное, при температуре 25-28°C, pH 6,8-7,2, постоянном освещении в 50-57 мкМ фотон/м2.с, в течение 14 дней.Технический результат состоит в повышении биосинтеза липидов и их накопления в биомассе микроводоросли Porphyridium cruentum.