Process for cultivating Spirulina platensis cyanobacterium

The invention relates to biotechnology, namely to a process for cultivating Spirulina platensis cyanobacterium and can be used for producing raw materials for the development and manufacture of drugs with anticancer and immunomodulatory action.According to the invention, the process for cultivating...

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Hauptverfasser: CHIRIAC Tatiana, VALUŢA Ana, ROTARI Ion, RUDI Liudmila, RUDIC Valeriu, MISCU Vera, DJUR Svetlana, DUMBRĂVEANU Veronica, CEPOI Anastasia, CEPOI Liliana, ZINICOVSCAIA Inga, TAŞCĂ Ion
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creator CHIRIAC Tatiana
VALUŢA Ana
ROTARI Ion
RUDI Liudmila
RUDIC Valeriu
MISCU Vera
DJUR Svetlana
DUMBRĂVEANU Veronica
CEPOI Anastasia
CEPOI Liliana
ZINICOVSCAIA Inga
TAŞCĂ Ion
description The invention relates to biotechnology, namely to a process for cultivating Spirulina platensis cyanobacterium and can be used for producing raw materials for the development and manufacture of drugs with anticancer and immunomodulatory action.According to the invention, the process for cultivating Spirulina platensis cyanobacterium comprises cultivation of cyanobacterium on a nutrient medium, containing, g/L: NaNO3-2,5; NaHCO3-8.0; NaCI-1.0; K2SO4-1.0; Na2HPO4-0.2; MgS04 ·7H2O-0.2, H3BO3-0.00286; MnCl2·4H2O-0.00181; CuSO4·5H2O-0.00008; MoO3-0. 000015, FeEDTA 1.0 ml/L and distilled water the rest, at a temperature of 30-32°C, pH 8.0-10.0 and illumination of 37-55 µM photons/m2/s in continuous regime, for 6 days, at the same time silver nanoparticles in a polyethylene glycol shell of a size of up to 5 nm, in a concentration of 0.10-0.12 µM/L are added into the nutrient medium on the fifth day of cultivation. The technical result consists in reducing the content of malondialdehyde in the spirulina biomass.. Invenţia se referă la biotehnologie, şi anume la un procedeu de cultivare a cianobacteriei Spirulina platensis şi poate fi aplicată în scopul obţinerii de materie primă pentru elaborarea şi fabricarea remediilor cu acţiune antimalignă şi imunomodulatoare.Conform invenţiei, procedeul de cultivare a cianobacteriei Spirulina platensis include cultivarea cianobacteriei pe mediul nutritiv ce conţine, în g/L: NaNO3-2,5, NaHCO3-8,0, NaCI-1,0, K2SO4-1,0, Na2HPO4-0,2, MgSO4·7H2O-0,2, H3BO3-0,00286, MnCI2·4H2O-0,00181, CuSO4·5H2O-0,00008; MoO3-0,000015, FeEDTA 1,0 ml/L şi apă distilată restul, la temperatura de 30-32°C, pH 8,0-10,0 şi iluminarea de 37-55 µM fotoni/m2.s în regim continuu, timp de 6 zile, totodată în a cincea zi de cultivare în mediul nutritiv se adaugă nanoparticule de Ag în înveliş de polietilenglicol cu dimensiunea de până la 5 nm în concentraţia de 0,10-0,12 µM/L.Rezultatul invenţiei constă în reducerea conţinutului dialdehidei malonice în biomasa de spirulină. Изобретение относится к биотехнологии, а именно к способу культивирования цианобактерии Spirulina platensis и может быть применено с целью получения сырья для разработки и производства средств с противораковым и иммуномодулирующим действием.Согласно изобретению, способ культивирования цианобактерии Spirulina platensis включает культивирование цианобактерии на питательной среде, содержащей, г/л: NaNO3-2,5, NaHCO3-8,0, NaCI-1,0, K2SO4-1,0, Na2HPO4-0,2, MgSO4·7H2O-0,2, H3BO3-0,00286, MnCI2·4H
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fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_MD4796B1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>MD4796B1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_MD4796B13</originalsourceid><addsrcrecordid>eNrjZLAKKMpPTi0uVkjLL1JILs0pySxLLMnMS1cILsgsKs3JzEtUKMhJLEnNK84sVkiuTMzLT0pMLkktyizN5WFgTUvMKU7lhdLcDLJuriHOHrqpBfnxqcUFicmpeakl8b4uJuaWZk6GxoTkAW2rLmE</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Process for cultivating Spirulina platensis cyanobacterium</title><source>esp@cenet</source><creator>CHIRIAC Tatiana ; VALUŢA Ana ; ROTARI Ion ; RUDI Liudmila ; RUDIC Valeriu ; MISCU Vera ; DJUR Svetlana ; DUMBRĂVEANU Veronica ; CEPOI Anastasia ; CEPOI Liliana ; ZINICOVSCAIA Inga ; TAŞCĂ Ion</creator><creatorcontrib>CHIRIAC Tatiana ; VALUŢA Ana ; ROTARI Ion ; RUDI Liudmila ; RUDIC Valeriu ; MISCU Vera ; DJUR Svetlana ; DUMBRĂVEANU Veronica ; CEPOI Anastasia ; CEPOI Liliana ; ZINICOVSCAIA Inga ; TAŞCĂ Ion</creatorcontrib><description>The invention relates to biotechnology, namely to a process for cultivating Spirulina platensis cyanobacterium and can be used for producing raw materials for the development and manufacture of drugs with anticancer and immunomodulatory action.According to the invention, the process for cultivating Spirulina platensis cyanobacterium comprises cultivation of cyanobacterium on a nutrient medium, containing, g/L: NaNO3-2,5; NaHCO3-8.0; NaCI-1.0; K2SO4-1.0; Na2HPO4-0.2; MgS04 ·7H2O-0.2, H3BO3-0.00286; MnCl2·4H2O-0.00181; CuSO4·5H2O-0.00008; MoO3-0. 000015, FeEDTA 1.0 ml/L and distilled water the rest, at a temperature of 30-32°C, pH 8.0-10.0 and illumination of 37-55 µM photons/m2/s in continuous regime, for 6 days, at the same time silver nanoparticles in a polyethylene glycol shell of a size of up to 5 nm, in a concentration of 0.10-0.12 µM/L are added into the nutrient medium on the fifth day of cultivation. The technical result consists in reducing the content of malondialdehyde in the spirulina biomass.. Invenţia se referă la biotehnologie, şi anume la un procedeu de cultivare a cianobacteriei Spirulina platensis şi poate fi aplicată în scopul obţinerii de materie primă pentru elaborarea şi fabricarea remediilor cu acţiune antimalignă şi imunomodulatoare.Conform invenţiei, procedeul de cultivare a cianobacteriei Spirulina platensis include cultivarea cianobacteriei pe mediul nutritiv ce conţine, în g/L: NaNO3-2,5, NaHCO3-8,0, NaCI-1,0, K2SO4-1,0, Na2HPO4-0,2, MgSO4·7H2O-0,2, H3BO3-0,00286, MnCI2·4H2O-0,00181, CuSO4·5H2O-0,00008; MoO3-0,000015, FeEDTA 1,0 ml/L şi apă distilată restul, la temperatura de 30-32°C, pH 8,0-10,0 şi iluminarea de 37-55 µM fotoni/m2.s în regim continuu, timp de 6 zile, totodată în a cincea zi de cultivare în mediul nutritiv se adaugă nanoparticule de Ag în înveliş de polietilenglicol cu dimensiunea de până la 5 nm în concentraţia de 0,10-0,12 µM/L.Rezultatul invenţiei constă în reducerea conţinutului dialdehidei malonice în biomasa de spirulină. Изобретение относится к биотехнологии, а именно к способу культивирования цианобактерии Spirulina platensis и может быть применено с целью получения сырья для разработки и производства средств с противораковым и иммуномодулирующим действием.Согласно изобретению, способ культивирования цианобактерии Spirulina platensis включает культивирование цианобактерии на питательной среде, содержащей, г/л: NaNO3-2,5, NaHCO3-8,0, NaCI-1,0, K2SO4-1,0, Na2HPO4-0,2, MgSO4·7H2O-0,2, H3BO3-0,00286, MnCI2·4H2O-0,00181, CuSO4·5H2O-0,00008; MoO3-0,000015, FeEDTA 1,0 мл/л и воду дистиллированную остальное, при температуре 30-32°C, pH 8,0-10,0, при постоянном освещении в 37-55 µM фотонов/м2.сек, в течение 6 дней, при этом на пятый день культивирования в питательную среду добавляют наночастицы серебра в полиэтиленгликолевой оболочке размером до 5 нм, в концентрации 0,10-0,12 µM/л. Технический результат заключается в снижении содержания малонового диальдегида в биомассе спирулины.</description><language>eng ; rum ; rus</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSESC01D OR C01F ; CULTURE MEDIA ; ENZYMOLOGY ; INORGANIC CHEMISTRY ; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES ; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NANOTECHNOLOGY ; PERFORMING OPERATIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES ; SPIRITS ; TRANSPORTING ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20220228&amp;DB=EPODOC&amp;CC=MD&amp;NR=4796B1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20220228&amp;DB=EPODOC&amp;CC=MD&amp;NR=4796B1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHIRIAC Tatiana</creatorcontrib><creatorcontrib>VALUŢA Ana</creatorcontrib><creatorcontrib>ROTARI Ion</creatorcontrib><creatorcontrib>RUDI Liudmila</creatorcontrib><creatorcontrib>RUDIC Valeriu</creatorcontrib><creatorcontrib>MISCU Vera</creatorcontrib><creatorcontrib>DJUR Svetlana</creatorcontrib><creatorcontrib>DUMBRĂVEANU Veronica</creatorcontrib><creatorcontrib>CEPOI Anastasia</creatorcontrib><creatorcontrib>CEPOI Liliana</creatorcontrib><creatorcontrib>ZINICOVSCAIA Inga</creatorcontrib><creatorcontrib>TAŞCĂ Ion</creatorcontrib><title>Process for cultivating Spirulina platensis cyanobacterium</title><description>The invention relates to biotechnology, namely to a process for cultivating Spirulina platensis cyanobacterium and can be used for producing raw materials for the development and manufacture of drugs with anticancer and immunomodulatory action.According to the invention, the process for cultivating Spirulina platensis cyanobacterium comprises cultivation of cyanobacterium on a nutrient medium, containing, g/L: NaNO3-2,5; NaHCO3-8.0; NaCI-1.0; K2SO4-1.0; Na2HPO4-0.2; MgS04 ·7H2O-0.2, H3BO3-0.00286; MnCl2·4H2O-0.00181; CuSO4·5H2O-0.00008; MoO3-0. 000015, FeEDTA 1.0 ml/L and distilled water the rest, at a temperature of 30-32°C, pH 8.0-10.0 and illumination of 37-55 µM photons/m2/s in continuous regime, for 6 days, at the same time silver nanoparticles in a polyethylene glycol shell of a size of up to 5 nm, in a concentration of 0.10-0.12 µM/L are added into the nutrient medium on the fifth day of cultivation. The technical result consists in reducing the content of malondialdehyde in the spirulina biomass.. Invenţia se referă la biotehnologie, şi anume la un procedeu de cultivare a cianobacteriei Spirulina platensis şi poate fi aplicată în scopul obţinerii de materie primă pentru elaborarea şi fabricarea remediilor cu acţiune antimalignă şi imunomodulatoare.Conform invenţiei, procedeul de cultivare a cianobacteriei Spirulina platensis include cultivarea cianobacteriei pe mediul nutritiv ce conţine, în g/L: NaNO3-2,5, NaHCO3-8,0, NaCI-1,0, K2SO4-1,0, Na2HPO4-0,2, MgSO4·7H2O-0,2, H3BO3-0,00286, MnCI2·4H2O-0,00181, CuSO4·5H2O-0,00008; MoO3-0,000015, FeEDTA 1,0 ml/L şi apă distilată restul, la temperatura de 30-32°C, pH 8,0-10,0 şi iluminarea de 37-55 µM fotoni/m2.s în regim continuu, timp de 6 zile, totodată în a cincea zi de cultivare în mediul nutritiv se adaugă nanoparticule de Ag în înveliş de polietilenglicol cu dimensiunea de până la 5 nm în concentraţia de 0,10-0,12 µM/L.Rezultatul invenţiei constă în reducerea conţinutului dialdehidei malonice în biomasa de spirulină. Изобретение относится к биотехнологии, а именно к способу культивирования цианобактерии Spirulina platensis и может быть применено с целью получения сырья для разработки и производства средств с противораковым и иммуномодулирующим действием.Согласно изобретению, способ культивирования цианобактерии Spirulina platensis включает культивирование цианобактерии на питательной среде, содержащей, г/л: NaNO3-2,5, NaHCO3-8,0, NaCI-1,0, K2SO4-1,0, Na2HPO4-0,2, MgSO4·7H2O-0,2, H3BO3-0,00286, MnCI2·4H2O-0,00181, CuSO4·5H2O-0,00008; MoO3-0,000015, FeEDTA 1,0 мл/л и воду дистиллированную остальное, при температуре 30-32°C, pH 8,0-10,0, при постоянном освещении в 37-55 µM фотонов/м2.сек, в течение 6 дней, при этом на пятый день культивирования в питательную среду добавляют наночастицы серебра в полиэтиленгликолевой оболочке размером до 5 нм, в концентрации 0,10-0,12 µM/л. Технический результат заключается в снижении содержания малонового диальдегида в биомассе спирулины.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSESC01D OR C01F</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INORGANIC CHEMISTRY</subject><subject>MANUFACTURE OR TREATMENT OF NANOSTRUCTURES</subject><subject>MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NANOTECHNOLOGY</subject><subject>PERFORMING OPERATIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES</subject><subject>SPIRITS</subject><subject>TRANSPORTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLAKKMpPTi0uVkjLL1JILs0pySxLLMnMS1cILsgsKs3JzEtUKMhJLEnNK84sVkiuTMzLT0pMLkktyizN5WFgTUvMKU7lhdLcDLJuriHOHrqpBfnxqcUFicmpeakl8b4uJuaWZk6GxoTkAW2rLmE</recordid><startdate>20220228</startdate><enddate>20220228</enddate><creator>CHIRIAC Tatiana</creator><creator>VALUŢA Ana</creator><creator>ROTARI Ion</creator><creator>RUDI Liudmila</creator><creator>RUDIC Valeriu</creator><creator>MISCU Vera</creator><creator>DJUR Svetlana</creator><creator>DUMBRĂVEANU Veronica</creator><creator>CEPOI Anastasia</creator><creator>CEPOI Liliana</creator><creator>ZINICOVSCAIA Inga</creator><creator>TAŞCĂ Ion</creator><scope>EVB</scope></search><sort><creationdate>20220228</creationdate><title>Process for cultivating Spirulina platensis cyanobacterium</title><author>CHIRIAC Tatiana ; VALUŢA Ana ; ROTARI Ion ; RUDI Liudmila ; RUDIC Valeriu ; MISCU Vera ; DJUR Svetlana ; DUMBRĂVEANU Veronica ; CEPOI Anastasia ; CEPOI Liliana ; ZINICOVSCAIA Inga ; TAŞCĂ Ion</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_MD4796B13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; rum ; rus</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSESC01D OR C01F</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INORGANIC CHEMISTRY</topic><topic>MANUFACTURE OR TREATMENT OF NANOSTRUCTURES</topic><topic>MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NANOTECHNOLOGY</topic><topic>PERFORMING OPERATIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES</topic><topic>SPIRITS</topic><topic>TRANSPORTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CHIRIAC Tatiana</creatorcontrib><creatorcontrib>VALUŢA Ana</creatorcontrib><creatorcontrib>ROTARI Ion</creatorcontrib><creatorcontrib>RUDI Liudmila</creatorcontrib><creatorcontrib>RUDIC Valeriu</creatorcontrib><creatorcontrib>MISCU Vera</creatorcontrib><creatorcontrib>DJUR Svetlana</creatorcontrib><creatorcontrib>DUMBRĂVEANU Veronica</creatorcontrib><creatorcontrib>CEPOI Anastasia</creatorcontrib><creatorcontrib>CEPOI Liliana</creatorcontrib><creatorcontrib>ZINICOVSCAIA Inga</creatorcontrib><creatorcontrib>TAŞCĂ Ion</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CHIRIAC Tatiana</au><au>VALUŢA Ana</au><au>ROTARI Ion</au><au>RUDI Liudmila</au><au>RUDIC Valeriu</au><au>MISCU Vera</au><au>DJUR Svetlana</au><au>DUMBRĂVEANU Veronica</au><au>CEPOI Anastasia</au><au>CEPOI Liliana</au><au>ZINICOVSCAIA Inga</au><au>TAŞCĂ Ion</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Process for cultivating Spirulina platensis cyanobacterium</title><date>2022-02-28</date><risdate>2022</risdate><abstract>The invention relates to biotechnology, namely to a process for cultivating Spirulina platensis cyanobacterium and can be used for producing raw materials for the development and manufacture of drugs with anticancer and immunomodulatory action.According to the invention, the process for cultivating Spirulina platensis cyanobacterium comprises cultivation of cyanobacterium on a nutrient medium, containing, g/L: NaNO3-2,5; NaHCO3-8.0; NaCI-1.0; K2SO4-1.0; Na2HPO4-0.2; MgS04 ·7H2O-0.2, H3BO3-0.00286; MnCl2·4H2O-0.00181; CuSO4·5H2O-0.00008; MoO3-0. 000015, FeEDTA 1.0 ml/L and distilled water the rest, at a temperature of 30-32°C, pH 8.0-10.0 and illumination of 37-55 µM photons/m2/s in continuous regime, for 6 days, at the same time silver nanoparticles in a polyethylene glycol shell of a size of up to 5 nm, in a concentration of 0.10-0.12 µM/L are added into the nutrient medium on the fifth day of cultivation. The technical result consists in reducing the content of malondialdehyde in the spirulina biomass.. Invenţia se referă la biotehnologie, şi anume la un procedeu de cultivare a cianobacteriei Spirulina platensis şi poate fi aplicată în scopul obţinerii de materie primă pentru elaborarea şi fabricarea remediilor cu acţiune antimalignă şi imunomodulatoare.Conform invenţiei, procedeul de cultivare a cianobacteriei Spirulina platensis include cultivarea cianobacteriei pe mediul nutritiv ce conţine, în g/L: NaNO3-2,5, NaHCO3-8,0, NaCI-1,0, K2SO4-1,0, Na2HPO4-0,2, MgSO4·7H2O-0,2, H3BO3-0,00286, MnCI2·4H2O-0,00181, CuSO4·5H2O-0,00008; MoO3-0,000015, FeEDTA 1,0 ml/L şi apă distilată restul, la temperatura de 30-32°C, pH 8,0-10,0 şi iluminarea de 37-55 µM fotoni/m2.s în regim continuu, timp de 6 zile, totodată în a cincea zi de cultivare în mediul nutritiv se adaugă nanoparticule de Ag în înveliş de polietilenglicol cu dimensiunea de până la 5 nm în concentraţia de 0,10-0,12 µM/L.Rezultatul invenţiei constă în reducerea conţinutului dialdehidei malonice în biomasa de spirulină. Изобретение относится к биотехнологии, а именно к способу культивирования цианобактерии Spirulina platensis и может быть применено с целью получения сырья для разработки и производства средств с противораковым и иммуномодулирующим действием.Согласно изобретению, способ культивирования цианобактерии Spirulina platensis включает культивирование цианобактерии на питательной среде, содержащей, г/л: NaNO3-2,5, NaHCO3-8,0, NaCI-1,0, K2SO4-1,0, Na2HPO4-0,2, MgSO4·7H2O-0,2, H3BO3-0,00286, MnCI2·4H2O-0,00181, CuSO4·5H2O-0,00008; MoO3-0,000015, FeEDTA 1,0 мл/л и воду дистиллированную остальное, при температуре 30-32°C, pH 8,0-10,0, при постоянном освещении в 37-55 µM фотонов/м2.сек, в течение 6 дней, при этом на пятый день культивирования в питательную среду добавляют наночастицы серебра в полиэтиленгликолевой оболочке размером до 5 нм, в концентрации 0,10-0,12 µM/л. Технический результат заключается в снижении содержания малонового диальдегида в биомассе спирулины.</abstract><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSESC01D OR C01F
CULTURE MEDIA
ENZYMOLOGY
INORGANIC CHEMISTRY
MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NANOTECHNOLOGY
PERFORMING OPERATIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
SPIRITS
TRANSPORTING
VINEGAR
WINE
title Process for cultivating Spirulina platensis cyanobacterium
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