Method for assessing the toxicity of nanoparticles by means of red microalga Porphyridium cruentum

The invention relates to nanobiotechnology, in particular to a method for assessing the toxicity of nanoparticles to algae and can be used as component part of the systems for monitoring the environment quality and safety of nanoparticle utilization and synthesis technological processes.The method,...

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Hauptverfasser:	CHIRIAC Tatiana, RUDI Liudmila, RUDIC Valeriu, MISCU Vera, SADOVNIC Daniela, CEPOI Liliana
Format:	Patent
Sprache:	eng ; rum ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NANOTECHNOLOGY PERFORMING OPERATIONS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to nanobiotechnology, in particular to a method for assessing the toxicity of nanoparticles to algae and can be used as component part of the systems for monitoring the environment quality and safety of nanoparticle utilization and synthesis technological processes.The method, according to the invention, comprises the cultivation of red microalga Porphyridium cruentum for 6 hours on a nutrient medium with addition in an hour after microalga inoculation of nanoparticles in different concentrations, afterwards in the alga biomass is determined the content of malonic dialdehyde, at the same time are considered toxic the concentrations of nanoparticles that cause the enhancement of malonic dialdehyde content in the biomass. Invenţia se referă la nanobiotehnologie, în special la un procedeu de apreciere a toxicităţii nanoparticulelor pentru microalgeşi poate fi utilizată în calitate de parte componentă a sistemelor de monitorizare a calităţii mediului şi a inofensivităţii proceselor tehnologice de sinteză şi utilizare a nanoparticulelor.Procedeul, conform invenţiei, include cultivarea microalgei roşii Porphyridium cruentum timp de 6 ore pe mediu nutritiv, cu adăugarea peste o oră după inocularea microalgei a nanoparticulelor în diferite concentraţii, după care în biomasa algală se determină conţinutul de dialdehidă malonică, totodată sunt considerate toxice concentraţiile de nanoparticule care provoacă creşterea conţinutului de dialdehidă malonică în biomasă. Изобретение относится к нанобиотехнологии, в частности к способу оценки токсичности наночастиц для микроводорослей и может быть использовано в качестве составной части систем мониторинга качества среды и безопасности технологических процессов синтеза и использования наночастиц.Способ, согласно изобретению, включает культивирование красной микроводоросли Porphyridium cruentum в течение 6 часов на питательной среде, с добавлением через час после инокуляции микроводоросли наночастиц в разных концентрациях, после чего в биомассе водоросли определяется количество малонового диальдегида, при этом считаются токсичными концентрации наночастиц которые способствуют повышению количества малонового диальдегида в биомассе.