Process for cultivation of cyanobacterium Spirulina platensis

The invention relates to biotechnology, in particular to a process for cultivation of cyanobacterium Spirulina platensis for the obtaining of biomass with high content of phycobiliproteins.The process for cultivation of cyanobacterium Spirulina platensis includes cultivation during 6 days on a nutri...

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Hauptverfasser: EFREMOVA NADEJDA, LOZAN VASILE, ELENCIUC DANIELA, BIVOL CEZARA, BULIMAGA VALENTINA, BOLOGA OLGA, RUDIC VALERIU, DJUR SVETLANA
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creator EFREMOVA NADEJDA
LOZAN VASILE
ELENCIUC DANIELA
BIVOL CEZARA
BULIMAGA VALENTINA
BOLOGA OLGA
RUDIC VALERIU
DJUR SVETLANA
description The invention relates to biotechnology, in particular to a process for cultivation of cyanobacterium Spirulina platensis for the obtaining of biomass with high content of phycobiliproteins.The process for cultivation of cyanobacterium Spirulina platensis includes cultivation during 6 days on a nutrient medium, containing, g/L: NaNO3 - 2.5, NaHCO3 - 16.8; NaCl - 1.0; K2SO4 - 1.0; K2HPO4·3H2O - 1.0; MgSO4·7H2O - 0.20; CaCl2·6H2O - 0.04; H3BO3 - 0.00286; MnCl2·4H2O - 0.00181; ZnSO4·7H2O - 0.00022; CuSO4·5H2O - 0.00008; MoO3 - 0.000015, 1 ml/L of FeHEDTA solution of 0.09 M and distilled water up to 1 L. On the first day of cultivation in the nutrient medium is added thiosemicarbazidediacetateethylenediamine Co(III) trihydrate with the formula ¢Co(L-H)En]·3H2O, where:L - H2N-CS-NH-N(CH2COOH) and En - H2N-C2H4-NH2, in the quantity of 10...20 mg/L, at the same time the cultivation is carried out at the light of 3000...4500 lx and the temperature of 25...30°C. Invenţia se referă la biotehnologie, în particular la un procedeu de cultivare a cianobacteriei Spirulina platensis pentru obţinerea biomasei cu un conţinut sporit de ficobiliproteine.Procedeul de cultivare a cianobacteriei Spirulina platensis include cultivarea timp de 6 zile pe un mediu nutritiv, conţinând, g/L: NaNO3 - 2,5; NaHCO3 - 16,8; NaCl - 1,0; K2SO4 - 1,0; K2HPO4·3H2O - 1,0; MgSO4·7H2O - 0,20; CaCl2·6H2O - 0,04; H3BO3 - 0,00286; MnCl2·4H2O - 0,00181; ZnSO4·7H2O - 0,00022; CuSO4·5H2O - 0,00008; MoO3 - 0,000015, 1mL/L de soluţie FeHEDTA de 0,09 M şi apă distilată până la 1 L. În prima zi de cultivare în mediul nutritiv se adaugă tiosemicarbaziddiacetatetilendiamina Co(III) trihidrat cu formula¢Co(L-H)En]·3H2O, unde:L - H2N-CS-NH-N(CH2COOH) şi En - H2N-C2H4-NH2, în cantitate de 10...20 mg/L, totodată cultivarea se efectuează la iluminarea de 3000...4500 lx şi temperatura de 25...30°C.
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On the first day of cultivation in the nutrient medium is added thiosemicarbazidediacetateethylenediamine Co(III) trihydrate with the formula ¢Co(L-H)En]·3H2O, where:L - H2N-CS-NH-N(CH2COOH) and En - H2N-C2H4-NH2, in the quantity of 10...20 mg/L, at the same time the cultivation is carried out at the light of 3000...4500 lx and the temperature of 25...30°C. Invenţia se referă la biotehnologie, în particular la un procedeu de cultivare a cianobacteriei Spirulina platensis pentru obţinerea biomasei cu un conţinut sporit de ficobiliproteine.Procedeul de cultivare a cianobacteriei Spirulina platensis include cultivarea timp de 6 zile pe un mediu nutritiv, conţinând, g/L: NaNO3 - 2,5; NaHCO3 - 16,8; NaCl - 1,0; K2SO4 - 1,0; K2HPO4·3H2O - 1,0; MgSO4·7H2O - 0,20; CaCl2·6H2O - 0,04; H3BO3 - 0,00286; MnCl2·4H2O - 0,00181; ZnSO4·7H2O - 0,00022; CuSO4·5H2O - 0,00008; MoO3 - 0,000015, 1mL/L de soluţie FeHEDTA de 0,09 M şi apă distilată până la 1 L. În prima zi de cultivare în mediul nutritiv se adaugă tiosemicarbaziddiacetatetilendiamina Co(III) trihidrat cu formula¢Co(L-H)En]·3H2O, unde:L - H2N-CS-NH-N(CH2COOH) şi En - H2N-C2H4-NH2, în cantitate de 10...20 mg/L, totodată cultivarea se efectuează la iluminarea de 3000...4500 lx şi temperatura de 25...30°C.</abstract><oa>free_for_read</oa></addata></record>
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subjects ACYCLIC OR CARBOCYCLIC COMPOUNDS
ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Process for cultivation of cyanobacterium Spirulina platensis
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