Method of obtaining cryoresistant bull seminal material
The invention relates to the veterinary medicine and can be used to increase the reproductive ability of bulls.The method of obtaining cryoresistant bull seminal material includes the intramuscular injection in the meddle part of the bull neck of 0.5% solution of Spirulina platensis extract in a dos...
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| creator | CHIRIAC Tatiana BUZAN Vitalii RUDIC Valeriu GRANACI Vera CEPOI Liliana |
| description | The invention relates to the veterinary medicine and can be used to increase the reproductive ability of bulls.The method of obtaining cryoresistant bull seminal material includes the intramuscular injection in the meddle part of the bull neck of 0.5% solution of Spirulina platensis extract in a dose of 0.4 ml/100 kg of body weight, during 10 days, after which it is carried out the selection and freezing of the seminal material using the extract obtained from Spirulina platensis extract cultivated on a medium, containing in g/L; NaNO3 - 2.5; NaHCO3 - 2.0; NaCl - 1.0; K2SO4 - 0.5; Na2HPO4 - 0.2; MgCl2 - 0.05; CaCl2 - 0.024; H2BO3 - 0.00286; MnCl2·4H2O - 0.00181; CuSO4·5H2O - 0.00008; MoO3 - 0.000015; Fe-EDTA - 1.0 ml and iron selenite, added in a concentration of 0.03 g/L in the first 12 hours of cultivation.
Invenţia se referă la medicina veterinară şi poate fi utilizată pentru sporirea capacităţii de reproducere a taurilor.Procedeul de obţinere a materialului seminal de tauri criorezistent include administrarea intramusculară în treimea mijlocie a gâtului taurului a unei soluţii de extract de Spirulina platensis de 0,5% în doză de 0,4 ml/100 kg de masă vie, timp de 10 zile, după care se efectuează recoltarea şi congelarea materialului seminal, totodată se utilizează extract obţinut din biomasă de Spirulina platensis cultivată pe un mediu, ce conţine în g/L: NaNO3 - 2,5; NaHCO3 - 2,0; NaCl - 1,0; K2SO4 - 0,5; Na2HPO4- 0,2; MgCl2 - 0,05; CaCl2 - 0,024; H2BO3 - 0,00286; MnCl2·4H2O - 0,00181; CuSO4·5H2O - 0,00008; MoO3 - 0,000015; Fe-EDTA - 1,0 ml şi selenit de fier, adaugat în concentraţie de 0,03 g/L în primele 12 ore de cultivare.
Изобретение относится к ветеринарии и может быть использовано для повышения воспроизводительной способности быков.Способ получения криорезистентного семенного материала быков включает внутримышечное введение в среднюю треть шеи быка 0,5%-ного раствора экстракта Spirulina platensis в дозе 0,4 мл/100 кг живой массы, в течение 10 дней, после чего осуществляют отбор и замораживание семенного материала, при этом используют экстракт полученный из биомассы Spirulina platensis культивированной на среде, содержащей г/л: NaNO3 - 2,5; NaHCO3 - 2,0; NaCl - 1,0; K2SO4 -0,5; Na2HPO4 - 0,2; MgCl2 - 0,05; CaCl2 - 0,024; H2BO3 - 0,00286; MnCl2·4H2O - 0,00181; CuSO4·5H2O - 0,00008; MoO3 - 0,000015; Fe-EDTA 1,0 мл и селенит железа, добавленный в концентрации 0,03 г/л в первые 12 часов культивирования. |
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Invenţia se referă la medicina veterinară şi poate fi utilizată pentru sporirea capacităţii de reproducere a taurilor.Procedeul de obţinere a materialului seminal de tauri criorezistent include administrarea intramusculară în treimea mijlocie a gâtului taurului a unei soluţii de extract de Spirulina platensis de 0,5% în doză de 0,4 ml/100 kg de masă vie, timp de 10 zile, după care se efectuează recoltarea şi congelarea materialului seminal, totodată se utilizează extract obţinut din biomasă de Spirulina platensis cultivată pe un mediu, ce conţine în g/L: NaNO3 - 2,5; NaHCO3 - 2,0; NaCl - 1,0; K2SO4 - 0,5; Na2HPO4- 0,2; MgCl2 - 0,05; CaCl2 - 0,024; H2BO3 - 0,00286; MnCl2·4H2O - 0,00181; CuSO4·5H2O - 0,00008; MoO3 - 0,000015; Fe-EDTA - 1,0 ml şi selenit de fier, adaugat în concentraţie de 0,03 g/L în primele 12 ore de cultivare.
Изобретение относится к ветеринарии и может быть использовано для повышения воспроизводительной способности быков.Способ получения криорезистентного семенного материала быков включает внутримышечное введение в среднюю треть шеи быка 0,5%-ного раствора экстракта Spirulina platensis в дозе 0,4 мл/100 кг живой массы, в течение 10 дней, после чего осуществляют отбор и замораживание семенного материала, при этом используют экстракт полученный из биомассы Spirulina platensis культивированной на среде, содержащей г/л: NaNO3 - 2,5; NaHCO3 - 2,0; NaCl - 1,0; K2SO4 -0,5; Na2HPO4 - 0,2; MgCl2 - 0,05; CaCl2 - 0,024; H2BO3 - 0,00286; MnCl2·4H2O - 0,00181; CuSO4·5H2O - 0,00008; MoO3 - 0,000015; Fe-EDTA 1,0 мл и селенит железа, добавленный в концентрации 0,03 г/л в первые 12 часов культивирования.</description><language>eng ; rum ; rus</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS ; VINEGAR ; WINE</subject><creationdate>2010</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20100630&DB=EPODOC&CC=MD&NR=221Y$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20100630&DB=EPODOC&CC=MD&NR=221Y$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHIRIAC Tatiana</creatorcontrib><creatorcontrib>BUZAN Vitalii</creatorcontrib><creatorcontrib>RUDIC Valeriu</creatorcontrib><creatorcontrib>GRANACI Vera</creatorcontrib><creatorcontrib>CEPOI Liliana</creatorcontrib><title>Method of obtaining cryoresistant bull seminal material</title><description>The invention relates to the veterinary medicine and can be used to increase the reproductive ability of bulls.The method of obtaining cryoresistant bull seminal material includes the intramuscular injection in the meddle part of the bull neck of 0.5% solution of Spirulina platensis extract in a dose of 0.4 ml/100 kg of body weight, during 10 days, after which it is carried out the selection and freezing of the seminal material using the extract obtained from Spirulina platensis extract cultivated on a medium, containing in g/L; NaNO3 - 2.5; NaHCO3 - 2.0; NaCl - 1.0; K2SO4 - 0.5; Na2HPO4 - 0.2; MgCl2 - 0.05; CaCl2 - 0.024; H2BO3 - 0.00286; MnCl2·4H2O - 0.00181; CuSO4·5H2O - 0.00008; MoO3 - 0.000015; Fe-EDTA - 1.0 ml and iron selenite, added in a concentration of 0.03 g/L in the first 12 hours of cultivation.
Invenţia se referă la medicina veterinară şi poate fi utilizată pentru sporirea capacităţii de reproducere a taurilor.Procedeul de obţinere a materialului seminal de tauri criorezistent include administrarea intramusculară în treimea mijlocie a gâtului taurului a unei soluţii de extract de Spirulina platensis de 0,5% în doză de 0,4 ml/100 kg de masă vie, timp de 10 zile, după care se efectuează recoltarea şi congelarea materialului seminal, totodată se utilizează extract obţinut din biomasă de Spirulina platensis cultivată pe un mediu, ce conţine în g/L: NaNO3 - 2,5; NaHCO3 - 2,0; NaCl - 1,0; K2SO4 - 0,5; Na2HPO4- 0,2; MgCl2 - 0,05; CaCl2 - 0,024; H2BO3 - 0,00286; MnCl2·4H2O - 0,00181; CuSO4·5H2O - 0,00008; MoO3 - 0,000015; Fe-EDTA - 1,0 ml şi selenit de fier, adaugat în concentraţie de 0,03 g/L în primele 12 ore de cultivare.
Изобретение относится к ветеринарии и может быть использовано для повышения воспроизводительной способности быков.Способ получения криорезистентного семенного материала быков включает внутримышечное введение в среднюю треть шеи быка 0,5%-ного раствора экстракта Spirulina platensis в дозе 0,4 мл/100 кг живой массы, в течение 10 дней, после чего осуществляют отбор и замораживание семенного материала, при этом используют экстракт полученный из биомассы Spirulina platensis культивированной на среде, содержащей г/л: NaNO3 - 2,5; NaHCO3 - 2,0; NaCl - 1,0; K2SO4 -0,5; Na2HPO4 - 0,2; MgCl2 - 0,05; CaCl2 - 0,024; H2BO3 - 0,00286; MnCl2·4H2O - 0,00181; CuSO4·5H2O - 0,00008; MoO3 - 0,000015; Fe-EDTA 1,0 мл и селенит железа, добавленный в концентрации 0,03 г/л в первые 12 часов культивирования.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</subject><subject>SPIRITS</subject><subject>VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2010</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDD3TS3JyE9RyE9TyE8qSczMy8xLV0guqswvSi3OLC5JzCtRSCrNyVEoTs3NzEvMUchNLEktykzM4WFgTUvMKU7lhdLcDDJuriHOHrqpBfnxqcUFicmpeakl8b4uRkaGkZHGBKQBTQYssg</recordid><startdate>20100630</startdate><enddate>20100630</enddate><creator>CHIRIAC Tatiana</creator><creator>BUZAN Vitalii</creator><creator>RUDIC Valeriu</creator><creator>GRANACI Vera</creator><creator>CEPOI Liliana</creator><scope>EVB</scope></search><sort><creationdate>20100630</creationdate><title>Method of obtaining cryoresistant bull seminal material</title><author>CHIRIAC Tatiana ; BUZAN Vitalii ; RUDIC Valeriu ; GRANACI Vera ; CEPOI Liliana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_MD221YY3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; rum ; rus</language><creationdate>2010</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</topic><topic>SPIRITS</topic><topic>VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CHIRIAC Tatiana</creatorcontrib><creatorcontrib>BUZAN Vitalii</creatorcontrib><creatorcontrib>RUDIC Valeriu</creatorcontrib><creatorcontrib>GRANACI Vera</creatorcontrib><creatorcontrib>CEPOI Liliana</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CHIRIAC Tatiana</au><au>BUZAN Vitalii</au><au>RUDIC Valeriu</au><au>GRANACI Vera</au><au>CEPOI Liliana</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method of obtaining cryoresistant bull seminal material</title><date>2010-06-30</date><risdate>2010</risdate><abstract>The invention relates to the veterinary medicine and can be used to increase the reproductive ability of bulls.The method of obtaining cryoresistant bull seminal material includes the intramuscular injection in the meddle part of the bull neck of 0.5% solution of Spirulina platensis extract in a dose of 0.4 ml/100 kg of body weight, during 10 days, after which it is carried out the selection and freezing of the seminal material using the extract obtained from Spirulina platensis extract cultivated on a medium, containing in g/L; NaNO3 - 2.5; NaHCO3 - 2.0; NaCl - 1.0; K2SO4 - 0.5; Na2HPO4 - 0.2; MgCl2 - 0.05; CaCl2 - 0.024; H2BO3 - 0.00286; MnCl2·4H2O - 0.00181; CuSO4·5H2O - 0.00008; MoO3 - 0.000015; Fe-EDTA - 1.0 ml and iron selenite, added in a concentration of 0.03 g/L in the first 12 hours of cultivation.
Invenţia se referă la medicina veterinară şi poate fi utilizată pentru sporirea capacităţii de reproducere a taurilor.Procedeul de obţinere a materialului seminal de tauri criorezistent include administrarea intramusculară în treimea mijlocie a gâtului taurului a unei soluţii de extract de Spirulina platensis de 0,5% în doză de 0,4 ml/100 kg de masă vie, timp de 10 zile, după care se efectuează recoltarea şi congelarea materialului seminal, totodată se utilizează extract obţinut din biomasă de Spirulina platensis cultivată pe un mediu, ce conţine în g/L: NaNO3 - 2,5; NaHCO3 - 2,0; NaCl - 1,0; K2SO4 - 0,5; Na2HPO4- 0,2; MgCl2 - 0,05; CaCl2 - 0,024; H2BO3 - 0,00286; MnCl2·4H2O - 0,00181; CuSO4·5H2O - 0,00008; MoO3 - 0,000015; Fe-EDTA - 1,0 ml şi selenit de fier, adaugat în concentraţie de 0,03 g/L în primele 12 ore de cultivare.
Изобретение относится к ветеринарии и может быть использовано для повышения воспроизводительной способности быков.Способ получения криорезистентного семенного материала быков включает внутримышечное введение в среднюю треть шеи быка 0,5%-ного раствора экстракта Spirulina platensis в дозе 0,4 мл/100 кг живой массы, в течение 10 дней, после чего осуществляют отбор и замораживание семенного материала, при этом используют экстракт полученный из биомассы Spirulina platensis культивированной на среде, содержащей г/л: NaNO3 - 2,5; NaHCO3 - 2,0; NaCl - 1,0; K2SO4 -0,5; Na2HPO4 - 0,2; MgCl2 - 0,05; CaCl2 - 0,024; H2BO3 - 0,00286; MnCl2·4H2O - 0,00181; CuSO4·5H2O - 0,00008; MoO3 - 0,000015; Fe-EDTA 1,0 мл и селенит железа, добавленный в концентрации 0,03 г/л в первые 12 часов культивирования.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS VINEGAR WINE |
| title | Method of obtaining cryoresistant bull seminal material |
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