Process for producing a proteoglycan preparation
The invention relates to biotechnology, in particular to the production of a proteoglycan preparation from the residual biomass of Arthrospira platensis cyanobacteria, which can be used in the zootechnical branch, food and cosmetic industries.The proteoglycan preparation, according to the invention,...
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creator | TOFAN Elena BEŞLIU Alina RUDIC Valeriu CHISELIŢA Oleg EFREMOVA Nadejda CHISELIŢA Natalia |
description | The invention relates to biotechnology, in particular to the production of a proteoglycan preparation from the residual biomass of Arthrospira platensis cyanobacteria, which can be used in the zootechnical branch, food and cosmetic industries.The proteoglycan preparation, according to the invention, is obtained from the residual biomass of Arthrospira platensis cyanobacteria, dried at a temperature of +50±5°C, which is ground for 3 min, mixed with 96% ethyl alcohol in a ratio of 1:10, the resulting suspension is placed in a water bath at a temperature of +45°C for 30 min, with periodic stirring, the extract is separated from the biomass by centrifugation at 3500 rpm for 5 min, then the biomass is mixed with distilled water in a ratio of 1:3, treated with ultrasound of a power of 50 W for 5 min and heated at a temperature of +45°C for 30 min or autoclaved at a temperature of +115°C (0.5 atm) for 30 min, the resulting extract is separated from the biomass by centrifugation at 3500 rpm for 10 min and is added 0. 2 mmol/L of EDTA to the resulting proteoglycan preparation.
Invenţia se referă la biotehnologie, în special la obţinerea unui preparat proteoglicanic din biomasa restantă de cianobacterii Arthrospira platensis, care poate fi utilizat în sectorul zootehnic, industria alimentară şi cosmetică.Preparatul proteoglicanic, conform invenţiei, se obţine din biomasa restantă de cianobacterii Arthrospira platensis,uscată la temperatura de +50±5°C, care se macină timp de 3 min, se amestecă cu alcool etilic de 96% în raport de 1:10, suspensia obţinută se plasează la baia cu apă la temperatura de +45°C timp de 30 de min, cu agitare periodică, extractul este separat de biomasă prin centrifugare la 3500 rot/min timp de 5 min, apoi biomasa se amestecă cu apă distilată în raport de 1:3, se tratează cu ultrasunet cu putere de 50 W timp de 5 min şi se încălzeşte la temperatura de +45°C timp de 30 de min sau se autoclavează la temperatura de +115°C (0,5 atm) timp de 30 de min, extractul obţinut se separă de biomasă prin centrifugare la 3500 rot/min timp de 10 min şi în preparatul proteoglicanic obţinut se adaugă şi 0,2 mmol/L EDTA.
Изобретение относится к биотехнологии, в частности к получению протеогликанового препарата из остаточной биомассы цианобактерий Arthrospira platensis, который может быть использован в зоотехнической отрасли, пищевой и косметической промышленности.Протеогликановый препарат, согласно изобретению, получают из остаточной биомассы цианобактерий Arth |
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fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_MD20220059A2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>MD20220059A2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_MD20220059A23</originalsourceid><addsrcrecordid>eNrjZDAIKMpPTi0uVkjLL1IoKMpPKU3OzEtXSASxS1Lz03MqkxPzgJzUgsSixJLM_DweBta0xJziVF4ozc2g6OYa4uyhm1qQH59aXJCYnJqXWhLv62JkYGRkYGBq6WhkTIwaAI7eK7E</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Process for producing a proteoglycan preparation</title><source>esp@cenet</source><creator>TOFAN Elena ; BEŞLIU Alina ; RUDIC Valeriu ; CHISELIŢA Oleg ; EFREMOVA Nadejda ; CHISELIŢA Natalia</creator><creatorcontrib>TOFAN Elena ; BEŞLIU Alina ; RUDIC Valeriu ; CHISELIŢA Oleg ; EFREMOVA Nadejda ; CHISELIŢA Natalia</creatorcontrib><description>The invention relates to biotechnology, in particular to the production of a proteoglycan preparation from the residual biomass of Arthrospira platensis cyanobacteria, which can be used in the zootechnical branch, food and cosmetic industries.The proteoglycan preparation, according to the invention, is obtained from the residual biomass of Arthrospira platensis cyanobacteria, dried at a temperature of +50±5°C, which is ground for 3 min, mixed with 96% ethyl alcohol in a ratio of 1:10, the resulting suspension is placed in a water bath at a temperature of +45°C for 30 min, with periodic stirring, the extract is separated from the biomass by centrifugation at 3500 rpm for 5 min, then the biomass is mixed with distilled water in a ratio of 1:3, treated with ultrasound of a power of 50 W for 5 min and heated at a temperature of +45°C for 30 min or autoclaved at a temperature of +115°C (0.5 atm) for 30 min, the resulting extract is separated from the biomass by centrifugation at 3500 rpm for 10 min and is added 0. 2 mmol/L of EDTA to the resulting proteoglycan preparation.
Invenţia se referă la biotehnologie, în special la obţinerea unui preparat proteoglicanic din biomasa restantă de cianobacterii Arthrospira platensis, care poate fi utilizat în sectorul zootehnic, industria alimentară şi cosmetică.Preparatul proteoglicanic, conform invenţiei, se obţine din biomasa restantă de cianobacterii Arthrospira platensis,uscată la temperatura de +50±5°C, care se macină timp de 3 min, se amestecă cu alcool etilic de 96% în raport de 1:10, suspensia obţinută se plasează la baia cu apă la temperatura de +45°C timp de 30 de min, cu agitare periodică, extractul este separat de biomasă prin centrifugare la 3500 rot/min timp de 5 min, apoi biomasa se amestecă cu apă distilată în raport de 1:3, se tratează cu ultrasunet cu putere de 50 W timp de 5 min şi se încălzeşte la temperatura de +45°C timp de 30 de min sau se autoclavează la temperatura de +115°C (0,5 atm) timp de 30 de min, extractul obţinut se separă de biomasă prin centrifugare la 3500 rot/min timp de 10 min şi în preparatul proteoglicanic obţinut se adaugă şi 0,2 mmol/L EDTA.
Изобретение относится к биотехнологии, в частности к получению протеогликанового препарата из остаточной биомассы цианобактерий Arthrospira platensis, который может быть использован в зоотехнической отрасли, пищевой и косметической промышленности.Протеогликановый препарат, согласно изобретению, получают из остаточной биомассы цианобактерий Arthrospira platensis, высушенной при температуре +50±5°С, которую растирают в течение 3 мин, смешивают с 96% этиловым спиртом в соотношении 1:10, полученную суспензию помещают на водяную баню при температуре +45°С в течение 30 мин, при периодическом перемешивании, экстракт отделяют от биомассы центрифугированием при 3500 об/мин в течение 5 мин, затем биомассу смешивают с дистиллированной водой в соотношении 1:3, обрабатывают ультразвуком мощностью 50 Вт в течение 5 мин и прогревают при температуре +45°С в течение 30 мин или автоклавируют при температуре +115°С (0,5 атм) в течение 30 мин, полученный экстракт отделяют от биомассы центрифугированием при 3500 об/мин в течение 10 мин и добавляют 0,2 ммоль/л ЭДТА к полученному протеогликановому препарату.</description><language>eng ; rum ; rus</language><subject>CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY ; FODDER ; FOODS OR FOODSTUFFS ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; SEPARATION ; THEIR RELEVANT APPARATUS ; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES ; TRANSPORTING</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240630&DB=EPODOC&CC=MD&NR=20220059A2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25544,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240630&DB=EPODOC&CC=MD&NR=20220059A2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TOFAN Elena</creatorcontrib><creatorcontrib>BEŞLIU Alina</creatorcontrib><creatorcontrib>RUDIC Valeriu</creatorcontrib><creatorcontrib>CHISELIŢA Oleg</creatorcontrib><creatorcontrib>EFREMOVA Nadejda</creatorcontrib><creatorcontrib>CHISELIŢA Natalia</creatorcontrib><title>Process for producing a proteoglycan preparation</title><description>The invention relates to biotechnology, in particular to the production of a proteoglycan preparation from the residual biomass of Arthrospira platensis cyanobacteria, which can be used in the zootechnical branch, food and cosmetic industries.The proteoglycan preparation, according to the invention, is obtained from the residual biomass of Arthrospira platensis cyanobacteria, dried at a temperature of +50±5°C, which is ground for 3 min, mixed with 96% ethyl alcohol in a ratio of 1:10, the resulting suspension is placed in a water bath at a temperature of +45°C for 30 min, with periodic stirring, the extract is separated from the biomass by centrifugation at 3500 rpm for 5 min, then the biomass is mixed with distilled water in a ratio of 1:3, treated with ultrasound of a power of 50 W for 5 min and heated at a temperature of +45°C for 30 min or autoclaved at a temperature of +115°C (0.5 atm) for 30 min, the resulting extract is separated from the biomass by centrifugation at 3500 rpm for 10 min and is added 0. 2 mmol/L of EDTA to the resulting proteoglycan preparation.
Invenţia se referă la biotehnologie, în special la obţinerea unui preparat proteoglicanic din biomasa restantă de cianobacterii Arthrospira platensis, care poate fi utilizat în sectorul zootehnic, industria alimentară şi cosmetică.Preparatul proteoglicanic, conform invenţiei, se obţine din biomasa restantă de cianobacterii Arthrospira platensis,uscată la temperatura de +50±5°C, care se macină timp de 3 min, se amestecă cu alcool etilic de 96% în raport de 1:10, suspensia obţinută se plasează la baia cu apă la temperatura de +45°C timp de 30 de min, cu agitare periodică, extractul este separat de biomasă prin centrifugare la 3500 rot/min timp de 5 min, apoi biomasa se amestecă cu apă distilată în raport de 1:3, se tratează cu ultrasunet cu putere de 50 W timp de 5 min şi se încălzeşte la temperatura de +45°C timp de 30 de min sau se autoclavează la temperatura de +115°C (0,5 atm) timp de 30 de min, extractul obţinut se separă de biomasă prin centrifugare la 3500 rot/min timp de 10 min şi în preparatul proteoglicanic obţinut se adaugă şi 0,2 mmol/L EDTA.
Изобретение относится к биотехнологии, в частности к получению протеогликанового препарата из остаточной биомассы цианобактерий Arthrospira platensis, который может быть использован в зоотехнической отрасли, пищевой и косметической промышленности.Протеогликановый препарат, согласно изобретению, получают из остаточной биомассы цианобактерий Arthrospira platensis, высушенной при температуре +50±5°С, которую растирают в течение 3 мин, смешивают с 96% этиловым спиртом в соотношении 1:10, полученную суспензию помещают на водяную баню при температуре +45°С в течение 30 мин, при периодическом перемешивании, экстракт отделяют от биомассы центрифугированием при 3500 об/мин в течение 5 мин, затем биомассу смешивают с дистиллированной водой в соотношении 1:3, обрабатывают ультразвуком мощностью 50 Вт в течение 5 мин и прогревают при температуре +45°С в течение 30 мин или автоклавируют при температуре +115°С (0,5 атм) в течение 30 мин, полученный экстракт отделяют от биомассы центрифугированием при 3500 об/мин в течение 10 мин и добавляют 0,2 ммоль/л ЭДТА к полученному протеогликановому препарату.</description><subject>CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY</subject><subject>FODDER</subject><subject>FOODS OR FOODSTUFFS</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>PERFORMING OPERATIONS</subject><subject>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>SEPARATION</subject><subject>THEIR RELEVANT APPARATUS</subject><subject>THEIR TREATMENT, NOT COVERED BY OTHER CLASSES</subject><subject>TRANSPORTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDAIKMpPTi0uVkjLL1IoKMpPKU3OzEtXSASxS1Lz03MqkxPzgJzUgsSixJLM_DweBta0xJziVF4ozc2g6OYa4uyhm1qQH59aXJCYnJqXWhLv62JkYGRkYGBq6WhkTIwaAI7eK7E</recordid><startdate>20240630</startdate><enddate>20240630</enddate><creator>TOFAN Elena</creator><creator>BEŞLIU Alina</creator><creator>RUDIC Valeriu</creator><creator>CHISELIŢA Oleg</creator><creator>EFREMOVA Nadejda</creator><creator>CHISELIŢA Natalia</creator><scope>EVB</scope></search><sort><creationdate>20240630</creationdate><title>Process for producing a proteoglycan preparation</title><author>TOFAN Elena ; BEŞLIU Alina ; RUDIC Valeriu ; CHISELIŢA Oleg ; EFREMOVA Nadejda ; CHISELIŢA Natalia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_MD20220059A23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; rum ; rus</language><creationdate>2024</creationdate><topic>CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY</topic><topic>FODDER</topic><topic>FOODS OR FOODSTUFFS</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>PERFORMING OPERATIONS</topic><topic>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>SEPARATION</topic><topic>THEIR RELEVANT APPARATUS</topic><topic>THEIR TREATMENT, NOT COVERED BY OTHER CLASSES</topic><topic>TRANSPORTING</topic><toplevel>online_resources</toplevel><creatorcontrib>TOFAN Elena</creatorcontrib><creatorcontrib>BEŞLIU Alina</creatorcontrib><creatorcontrib>RUDIC Valeriu</creatorcontrib><creatorcontrib>CHISELIŢA Oleg</creatorcontrib><creatorcontrib>EFREMOVA Nadejda</creatorcontrib><creatorcontrib>CHISELIŢA Natalia</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TOFAN Elena</au><au>BEŞLIU Alina</au><au>RUDIC Valeriu</au><au>CHISELIŢA Oleg</au><au>EFREMOVA Nadejda</au><au>CHISELIŢA Natalia</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Process for producing a proteoglycan preparation</title><date>2024-06-30</date><risdate>2024</risdate><abstract>The invention relates to biotechnology, in particular to the production of a proteoglycan preparation from the residual biomass of Arthrospira platensis cyanobacteria, which can be used in the zootechnical branch, food and cosmetic industries.The proteoglycan preparation, according to the invention, is obtained from the residual biomass of Arthrospira platensis cyanobacteria, dried at a temperature of +50±5°C, which is ground for 3 min, mixed with 96% ethyl alcohol in a ratio of 1:10, the resulting suspension is placed in a water bath at a temperature of +45°C for 30 min, with periodic stirring, the extract is separated from the biomass by centrifugation at 3500 rpm for 5 min, then the biomass is mixed with distilled water in a ratio of 1:3, treated with ultrasound of a power of 50 W for 5 min and heated at a temperature of +45°C for 30 min or autoclaved at a temperature of +115°C (0.5 atm) for 30 min, the resulting extract is separated from the biomass by centrifugation at 3500 rpm for 10 min and is added 0. 2 mmol/L of EDTA to the resulting proteoglycan preparation.
Invenţia se referă la biotehnologie, în special la obţinerea unui preparat proteoglicanic din biomasa restantă de cianobacterii Arthrospira platensis, care poate fi utilizat în sectorul zootehnic, industria alimentară şi cosmetică.Preparatul proteoglicanic, conform invenţiei, se obţine din biomasa restantă de cianobacterii Arthrospira platensis,uscată la temperatura de +50±5°C, care se macină timp de 3 min, se amestecă cu alcool etilic de 96% în raport de 1:10, suspensia obţinută se plasează la baia cu apă la temperatura de +45°C timp de 30 de min, cu agitare periodică, extractul este separat de biomasă prin centrifugare la 3500 rot/min timp de 5 min, apoi biomasa se amestecă cu apă distilată în raport de 1:3, se tratează cu ultrasunet cu putere de 50 W timp de 5 min şi se încălzeşte la temperatura de +45°C timp de 30 de min sau se autoclavează la temperatura de +115°C (0,5 atm) timp de 30 de min, extractul obţinut se separă de biomasă prin centrifugare la 3500 rot/min timp de 10 min şi în preparatul proteoglicanic obţinut se adaugă şi 0,2 mmol/L EDTA.
Изобретение относится к биотехнологии, в частности к получению протеогликанового препарата из остаточной биомассы цианобактерий Arthrospira platensis, который может быть использован в зоотехнической отрасли, пищевой и косметической промышленности.Протеогликановый препарат, согласно изобретению, получают из остаточной биомассы цианобактерий Arthrospira platensis, высушенной при температуре +50±5°С, которую растирают в течение 3 мин, смешивают с 96% этиловым спиртом в соотношении 1:10, полученную суспензию помещают на водяную баню при температуре +45°С в течение 30 мин, при периодическом перемешивании, экстракт отделяют от биомассы центрифугированием при 3500 об/мин в течение 5 мин, затем биомассу смешивают с дистиллированной водой в соотношении 1:3, обрабатывают ультразвуком мощностью 50 Вт в течение 5 мин и прогревают при температуре +45°С в течение 30 мин или автоклавируют при температуре +115°С (0,5 атм) в течение 30 мин, полученный экстракт отделяют от биомассы центрифугированием при 3500 об/мин в течение 10 мин и добавляют 0,2 ммоль/л ЭДТА к полученному протеогликановому препарату.</abstract><oa>free_for_read</oa></addata></record> |
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title | Process for producing a proteoglycan preparation |
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