Process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases
The invention relates to biotechnology, namely to a process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases and can be used in the microbiological industry for producing lipolytic enzymes with wide application in the food industry, production and processi...
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| creator | TIURINA Janeta DVORNINA Elena BULHAC Ion CLAPCO Steliana URECHE Dumitru LABLIUC Svetlana CILOCI Alexandra |
| description | The invention relates to biotechnology, namely to a process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases and can be used in the microbiological industry for producing lipolytic enzymes with wide application in the food industry, production and processing of fats and vegetable oils, in medicine in as a therapeutic and diagnostic agent.The process, according to the invention, provides for the production of a spore suspension of a strain grown for 30 days on a malt-agar medium, inoculation of the suspension in an amount of 5vol.% into a nutrient aqueous medium containing, g/L: soy flour - 35.0, (NH4)2SO4 - 1.0, KH2PO4 - 5.0, with simultaneous introduction of 0.005 g/L of [SrL3][Co(NCS)4], where L - dimethylpyridine-2,6-dicarboxylate, and cultivation with constant stirring at 180-200 rpm for 24 hours, at a temperature of 28-30°C.The result of the invention consists in increasing the biosynthesis of lipolytic enzymes and reducing the duration of cultivation of the strain by 24 hours.
Invenţia se referă la biotehnologie, şi anume la un procedeu de cultivare submersă a tulpinii de fungi Rhizopus arrhizus CNMN FD 03, producătoare de lipaze şi poate fi utilizată în industria microbiologică pentru producerea enzimelor lipolitice cu aplicare largă în industria alimentară, de producere şi procesare a grăsimilor şi uleiurilor vegetale, în medicină ca mijloc terapeutic şi diagnostic. Procedeul, conform invenţiei prevede obţinerea suspensiei de spori a tulpinii crescute timp de 30 zile pe un mediu de malţ-agar, inocularea suspensiei în cantitate de 5% v/v într-un mediu nutritiv apos ce conţine, g/L: făină de soia - 35,0, (NH4)2SO4 - 1,0, KH2PO4 - 5,0, cu adăugarea concomitentă a 0,005 g/L de [SrL3][Co(NCS)4], unde L - dimetilpiridin-2,6-dicarboxilat, şi cultivarea la agitare continuă de 180-200 rot/min în decurs de 24 de ore la temperatura de 28-30°C.Rezultatul invenţiei constă în sporirea biosintezei enzimelor lipolitice şi reducerea duratei de cultivare a tulpinii cu 24 de ore.
Изобретение относится к биотехнологии, а именно к способу глубинного культивирования штамма гриба Rhizopus arrhizus CNMN FD 03, продуцента липаз и может быть использовано в микробиологической промышленности для получения липолитических ферментов с широким применением в пищевой промышленности, производстве и переработке жиров и растительных масел, в медицине в качестве терапевтического и диагностического средства.Способ, согласно изобретению, предус |
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Invenţia se referă la biotehnologie, şi anume la un procedeu de cultivare submersă a tulpinii de fungi Rhizopus arrhizus CNMN FD 03, producătoare de lipaze şi poate fi utilizată în industria microbiologică pentru producerea enzimelor lipolitice cu aplicare largă în industria alimentară, de producere şi procesare a grăsimilor şi uleiurilor vegetale, în medicină ca mijloc terapeutic şi diagnostic. Procedeul, conform invenţiei prevede obţinerea suspensiei de spori a tulpinii crescute timp de 30 zile pe un mediu de malţ-agar, inocularea suspensiei în cantitate de 5% v/v într-un mediu nutritiv apos ce conţine, g/L: făină de soia - 35,0, (NH4)2SO4 - 1,0, KH2PO4 - 5,0, cu adăugarea concomitentă a 0,005 g/L de [SrL3][Co(NCS)4], unde L - dimetilpiridin-2,6-dicarboxilat, şi cultivarea la agitare continuă de 180-200 rot/min în decurs de 24 de ore la temperatura de 28-30°C.Rezultatul invenţiei constă în sporirea biosintezei enzimelor lipolitice şi reducerea duratei de cultivare a tulpinii cu 24 de ore.
Изобретение относится к биотехнологии, а именно к способу глубинного культивирования штамма гриба Rhizopus arrhizus CNMN FD 03, продуцента липаз и может быть использовано в микробиологической промышленности для получения липолитических ферментов с широким применением в пищевой промышленности, производстве и переработке жиров и растительных масел, в медицине в качестве терапевтического и диагностического средства.Способ, согласно изобретению, предусматривает получение суспензии спор штамма выращенного в течении 30 дней на среде солод-агар, инокуляцию суспензии в количестве 5 об.% в питательную водную среду, содержащую, г/л: соевую муку - 35,0, (NH4)2SO4 - 1,0, KH2PO4 -5,0, с одновременным введением 0,005 г/л [SrL3][Co(NCS)4], где L - диметилпиридин-2,6-дикарбоксилат, и культивирование при постоянном перемешивания при 180-200 об/мин в течение 24 ч, при температуре 28-30°C.Результат изобретения состоит в увеличении биосинтеза липолитических ферментов и сокращении продолжительности культивирования штамма на 24 часа.</description><language>eng ; rum ; rus</language><subject>ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; HETEROCYCLIC COMPOUNDS ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240131&DB=EPODOC&CC=MD&NR=1624Z8$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240131&DB=EPODOC&CC=MD&NR=1624Z8$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TIURINA Janeta</creatorcontrib><creatorcontrib>DVORNINA Elena</creatorcontrib><creatorcontrib>BULHAC Ion</creatorcontrib><creatorcontrib>CLAPCO Steliana</creatorcontrib><creatorcontrib>URECHE Dumitru</creatorcontrib><creatorcontrib>LABLIUC Svetlana</creatorcontrib><creatorcontrib>CILOCI Alexandra</creatorcontrib><title>Process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases</title><description>The invention relates to biotechnology, namely to a process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases and can be used in the microbiological industry for producing lipolytic enzymes with wide application in the food industry, production and processing of fats and vegetable oils, in medicine in as a therapeutic and diagnostic agent.The process, according to the invention, provides for the production of a spore suspension of a strain grown for 30 days on a malt-agar medium, inoculation of the suspension in an amount of 5vol.% into a nutrient aqueous medium containing, g/L: soy flour - 35.0, (NH4)2SO4 - 1.0, KH2PO4 - 5.0, with simultaneous introduction of 0.005 g/L of [SrL3][Co(NCS)4], where L - dimethylpyridine-2,6-dicarboxylate, and cultivation with constant stirring at 180-200 rpm for 24 hours, at a temperature of 28-30°C.The result of the invention consists in increasing the biosynthesis of lipolytic enzymes and reducing the duration of cultivation of the strain by 24 hours.
Invenţia se referă la biotehnologie, şi anume la un procedeu de cultivare submersă a tulpinii de fungi Rhizopus arrhizus CNMN FD 03, producătoare de lipaze şi poate fi utilizată în industria microbiologică pentru producerea enzimelor lipolitice cu aplicare largă în industria alimentară, de producere şi procesare a grăsimilor şi uleiurilor vegetale, în medicină ca mijloc terapeutic şi diagnostic. Procedeul, conform invenţiei prevede obţinerea suspensiei de spori a tulpinii crescute timp de 30 zile pe un mediu de malţ-agar, inocularea suspensiei în cantitate de 5% v/v într-un mediu nutritiv apos ce conţine, g/L: făină de soia - 35,0, (NH4)2SO4 - 1,0, KH2PO4 - 5,0, cu adăugarea concomitentă a 0,005 g/L de [SrL3][Co(NCS)4], unde L - dimetilpiridin-2,6-dicarboxilat, şi cultivarea la agitare continuă de 180-200 rot/min în decurs de 24 de ore la temperatura de 28-30°C.Rezultatul invenţiei constă în sporirea biosintezei enzimelor lipolitice şi reducerea duratei de cultivare a tulpinii cu 24 de ore.
Изобретение относится к биотехнологии, а именно к способу глубинного культивирования штамма гриба Rhizopus arrhizus CNMN FD 03, продуцента липаз и может быть использовано в микробиологической промышленности для получения липолитических ферментов с широким применением в пищевой промышленности, производстве и переработке жиров и растительных масел, в медицине в качестве терапевтического и диагностического средства.Способ, согласно изобретению, предусматривает получение суспензии спор штамма выращенного в течении 30 дней на среде солод-агар, инокуляцию суспензии в количестве 5 об.% в питательную водную среду, содержащую, г/л: соевую муку - 35,0, (NH4)2SO4 - 1,0, KH2PO4 -5,0, с одновременным введением 0,005 г/л [SrL3][Co(NCS)4], где L - диметилпиридин-2,6-дикарбоксилат, и культивирование при постоянном перемешивания при 180-200 об/мин в течение 24 ч, при температуре 28-30°C.Результат изобретения состоит в увеличении биосинтеза липолитических ферментов и сокращении продолжительности культивирования штамма на 24 часа.</description><subject>ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HETEROCYCLIC COMPOUNDS</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqFi7EKwjAURbs4iPoL8j5AQa2Ie2txaRFx6lJi8lIDNS-8lzj49VZwdzqHw73TzFyYNIqAJQZJ9ydyjwZ0GqJ7qejIA1m4PtybQhJQzKOOUjR1A1UJmxxs8v1YJLJyfgWBySSN_P0NLihBmWcTqwbBxY-zbFmdbsV5jYE6lKA0eoxdXW4Pu33bHvO_gw8gFD00</recordid><startdate>20240131</startdate><enddate>20240131</enddate><creator>TIURINA Janeta</creator><creator>DVORNINA Elena</creator><creator>BULHAC Ion</creator><creator>CLAPCO Steliana</creator><creator>URECHE Dumitru</creator><creator>LABLIUC Svetlana</creator><creator>CILOCI Alexandra</creator><scope>EVB</scope></search><sort><creationdate>20240131</creationdate><title>Process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases</title><author>TIURINA Janeta ; DVORNINA Elena ; BULHAC Ion ; CLAPCO Steliana ; URECHE Dumitru ; LABLIUC Svetlana ; CILOCI Alexandra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_MD1624ZZ83</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; rum ; rus</language><creationdate>2024</creationdate><topic>ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HETEROCYCLIC COMPOUNDS</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>TIURINA Janeta</creatorcontrib><creatorcontrib>DVORNINA Elena</creatorcontrib><creatorcontrib>BULHAC Ion</creatorcontrib><creatorcontrib>CLAPCO Steliana</creatorcontrib><creatorcontrib>URECHE Dumitru</creatorcontrib><creatorcontrib>LABLIUC Svetlana</creatorcontrib><creatorcontrib>CILOCI Alexandra</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TIURINA Janeta</au><au>DVORNINA Elena</au><au>BULHAC Ion</au><au>CLAPCO Steliana</au><au>URECHE Dumitru</au><au>LABLIUC Svetlana</au><au>CILOCI Alexandra</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases</title><date>2024-01-31</date><risdate>2024</risdate><abstract>The invention relates to biotechnology, namely to a process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases and can be used in the microbiological industry for producing lipolytic enzymes with wide application in the food industry, production and processing of fats and vegetable oils, in medicine in as a therapeutic and diagnostic agent.The process, according to the invention, provides for the production of a spore suspension of a strain grown for 30 days on a malt-agar medium, inoculation of the suspension in an amount of 5vol.% into a nutrient aqueous medium containing, g/L: soy flour - 35.0, (NH4)2SO4 - 1.0, KH2PO4 - 5.0, with simultaneous introduction of 0.005 g/L of [SrL3][Co(NCS)4], where L - dimethylpyridine-2,6-dicarboxylate, and cultivation with constant stirring at 180-200 rpm for 24 hours, at a temperature of 28-30°C.The result of the invention consists in increasing the biosynthesis of lipolytic enzymes and reducing the duration of cultivation of the strain by 24 hours.
Invenţia se referă la biotehnologie, şi anume la un procedeu de cultivare submersă a tulpinii de fungi Rhizopus arrhizus CNMN FD 03, producătoare de lipaze şi poate fi utilizată în industria microbiologică pentru producerea enzimelor lipolitice cu aplicare largă în industria alimentară, de producere şi procesare a grăsimilor şi uleiurilor vegetale, în medicină ca mijloc terapeutic şi diagnostic. Procedeul, conform invenţiei prevede obţinerea suspensiei de spori a tulpinii crescute timp de 30 zile pe un mediu de malţ-agar, inocularea suspensiei în cantitate de 5% v/v într-un mediu nutritiv apos ce conţine, g/L: făină de soia - 35,0, (NH4)2SO4 - 1,0, KH2PO4 - 5,0, cu adăugarea concomitentă a 0,005 g/L de [SrL3][Co(NCS)4], unde L - dimetilpiridin-2,6-dicarboxilat, şi cultivarea la agitare continuă de 180-200 rot/min în decurs de 24 de ore la temperatura de 28-30°C.Rezultatul invenţiei constă în sporirea biosintezei enzimelor lipolitice şi reducerea duratei de cultivare a tulpinii cu 24 de ore.
Изобретение относится к биотехнологии, а именно к способу глубинного культивирования штамма гриба Rhizopus arrhizus CNMN FD 03, продуцента липаз и может быть использовано в микробиологической промышленности для получения липолитических ферментов с широким применением в пищевой промышленности, производстве и переработке жиров и растительных масел, в медицине в качестве терапевтического и диагностического средства.Способ, согласно изобретению, предусматривает получение суспензии спор штамма выращенного в течении 30 дней на среде солод-агар, инокуляцию суспензии в количестве 5 об.% в питательную водную среду, содержащую, г/л: соевую муку - 35,0, (NH4)2SO4 - 1,0, KH2PO4 -5,0, с одновременным введением 0,005 г/л [SrL3][Co(NCS)4], где L - диметилпиридин-2,6-дикарбоксилат, и культивирование при постоянном перемешивания при 180-200 об/мин в течение 24 ч, при температуре 28-30°C.Результат изобретения состоит в увеличении биосинтеза липолитических ферментов и сокращении продолжительности культивирования штамма на 24 часа.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HETEROCYCLIC COMPOUNDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases |
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