PROCESS FOR PREPARING "ANTIBIOTICS A/16686"

PROCESS FOR PREPARING "ANTIBIOTICS A/16686"

Antibiotic Al6686 is prepd. by fermn. with Actinoplanes ATCC 33076. Thus, a preculture was inoculated into 10l medium contg. meat ext. 4, peptone 4, yeast ext. 1, NaCl 2.5, soybean meal 10, glucose 25, and CaCO3 5g/l and incubated at 28-30 C with aeration and stirring until activity was max. in 3-5...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: PAGANI HERMES, VOLPE GIANCARLO, CAVALLEI BRUNO
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Antibiotic Al6686 is prepd. by fermn. with Actinoplanes ATCC 33076. Thus, a preculture was inoculated into 10l medium contg. meat ext. 4, peptone 4, yeast ext. 1, NaCl 2.5, soybean meal 10, glucose 25, and CaCO3 5g/l and incubated at 28-30 C with aeration and stirring until activity was max. in 3-5 days. The mycelium from 170l medium was extd. twice with MeOH. The exts. were concd. from 60 to 6land extd. with BuOH. The BuOH exts. were concd. and added to petroleum ethers. The ppt. contained 80 g Al6686, which was worked up by extn. and chromatog. steps to yield 1.875 g of pure antibiotic. Al6686 is glycopeptide active against gram-pos. bacteria.