전기화학발광 검출 방법

본 개시내용은 전기화학발광(ECL) 공반응물을 포함하는 신규 조성물에 관한 것이다. 일 실시형태에서, 본 조성물은 이온 성분, 계면활성제, 또는 이들의 조합을 더 포함한다. 일 실시형태에서, ECL 공반응물은 트라이에탄올아민(TEA), tert-뷰틸다이에탄올아민(tBDEA), 메틸다이뷰틸에탄올아민(MDEA), 3-[비스-(2-하이드록시-에틸)-아미노]-프로판-1-설폰산(DEA-PS), 또는 이들의 조합이다. 소광 모이어티를 갖는 ECL-표지 올리고뉴클레오타이드 프로브를 사용하는 방법을 비롯하여, 상기 조성물을 사용하는 방법 및 상기...

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Hauptverfasser: PETTERSSON JOHN, TUCKER SCHWARTZ ALEXANDER K, KENTEN JOHN, SIGAL GEORGE
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creator PETTERSSON JOHN
TUCKER SCHWARTZ ALEXANDER K
KENTEN JOHN
SIGAL GEORGE
description 본 개시내용은 전기화학발광(ECL) 공반응물을 포함하는 신규 조성물에 관한 것이다. 일 실시형태에서, 본 조성물은 이온 성분, 계면활성제, 또는 이들의 조합을 더 포함한다. 일 실시형태에서, ECL 공반응물은 트라이에탄올아민(TEA), tert-뷰틸다이에탄올아민(tBDEA), 메틸다이뷰틸에탄올아민(MDEA), 3-[비스-(2-하이드록시-에틸)-아미노]-프로판-1-설폰산(DEA-PS), 또는 이들의 조합이다. 소광 모이어티를 갖는 ECL-표지 올리고뉴클레오타이드 프로브를 사용하는 방법을 비롯하여, 상기 조성물을 사용하는 방법 및 상기 조성물을 포함하는 키트도 본 명세서에 제공되어 있다. The disclosure relates to novel compositions comprising an electrochemiluminescence (ECL) co-reactant. In embodiments, the composition further comprises an ionic component, a surfactant, or combination thereof. In embodiments, the ECL co-reactant is triethanolamine (TEA), tert-butyldiethanolamine (tBDEA), methyldibutylethanolamine (MDEA), 3-[Bis-(2-hydroxy-ethyl)-amino]-propane-1-sulfonic acid (DEA-PS), or a combination thereof. Methods of using the compositions and kits comprising the compositions are also provided herein, including methods using ECL-labeled oligonucleotide probes having quenching moieties.
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The disclosure relates to novel compositions comprising an electrochemiluminescence (ECL) co-reactant. In embodiments, the composition further comprises an ionic component, a surfactant, or combination thereof. In embodiments, the ECL co-reactant is triethanolamine (TEA), tert-butyldiethanolamine (tBDEA), methyldibutylethanolamine (MDEA), 3-[Bis-(2-hydroxy-ethyl)-amino]-propane-1-sulfonic acid (DEA-PS), or a combination thereof. 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The disclosure relates to novel compositions comprising an electrochemiluminescence (ECL) co-reactant. In embodiments, the composition further comprises an ionic component, a surfactant, or combination thereof. In embodiments, the ECL co-reactant is triethanolamine (TEA), tert-butyldiethanolamine (tBDEA), methyldibutylethanolamine (MDEA), 3-[Bis-(2-hydroxy-ethyl)-amino]-propane-1-sulfonic acid (DEA-PS), or a combination thereof. 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The disclosure relates to novel compositions comprising an electrochemiluminescence (ECL) co-reactant. In embodiments, the composition further comprises an ionic component, a surfactant, or combination thereof. In embodiments, the ECL co-reactant is triethanolamine (TEA), tert-butyldiethanolamine (tBDEA), methyldibutylethanolamine (MDEA), 3-[Bis-(2-hydroxy-ethyl)-amino]-propane-1-sulfonic acid (DEA-PS), or a combination thereof. Methods of using the compositions and kits comprising the compositions are also provided herein, including methods using ECL-labeled oligonucleotide probes having quenching moieties.</abstract><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
title 전기화학발광 검출 방법
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