Method for Expressing Target Protein
The present invention relates to a method for expressing a target protein, and more specifically, to a signal peptide for secreting the target protein into periplasm, a nucleic acid encoding the same, an expression vector containing the nucleic acid, a recombinant microorganism into which the expres...
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| creator | LA WAN GEUN RYU DONG KYUN KIM MAN SU LEE YOO WON |
| description | The present invention relates to a method for expressing a target protein, and more specifically, to a signal peptide for secreting the target protein into periplasm, a nucleic acid encoding the same, an expression vector containing the nucleic acid, a recombinant microorganism into which the expression vector has been introduced, and a method for expressing the target protein using the recombinant microorganism. The present invention not only ensures that the target protein is well expressed in E. coli, but also improves secretion efficiency into the periplasmic. Accordingly, high-yield protein production is possible through the target protein expression method according to the present invention.
본 발명은 목적 단백질의 발현 방법에 관한 것으로, 더욱 상세하게는 목적 단백질을 주변 세포질(periplasmic)로 분비시키기 위한 신호 펩티드, 이를 코딩하는 핵산, 이 핵산을 포함하는 발현 벡터, 이 발현 벡터가 도입되어 있는 재조합 미생물, 및 이 재조합 미생물을 이용한 목적 단백질의 발현 방법에 관한 것이다. 본 발명은 목적 단백질이 대장균에서 잘 발현되도록 할 뿐만 아니라 주변 세포질(periplasmic)로의 분비 효율이 향상되도록 한다. 이에 본 발명에 따른 목적 단백질 발현 방법을 통해 높은 수율의 단백질 생산이 가능하다. |
| format | Patent |
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본 발명은 목적 단백질의 발현 방법에 관한 것으로, 더욱 상세하게는 목적 단백질을 주변 세포질(periplasmic)로 분비시키기 위한 신호 펩티드, 이를 코딩하는 핵산, 이 핵산을 포함하는 발현 벡터, 이 발현 벡터가 도입되어 있는 재조합 미생물, 및 이 재조합 미생물을 이용한 목적 단백질의 발현 방법에 관한 것이다. 본 발명은 목적 단백질이 대장균에서 잘 발현되도록 할 뿐만 아니라 주변 세포질(periplasmic)로의 분비 효율이 향상되도록 한다. 이에 본 발명에 따른 목적 단백질 발현 방법을 통해 높은 수율의 단백질 생산이 가능하다.</description><language>eng ; kor</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230707&DB=EPODOC&CC=KR&NR=20230102682A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230707&DB=EPODOC&CC=KR&NR=20230102682A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LA WAN GEUN</creatorcontrib><creatorcontrib>RYU DONG KYUN</creatorcontrib><creatorcontrib>KIM MAN SU</creatorcontrib><creatorcontrib>LEE YOO WON</creatorcontrib><title>Method for Expressing Target Protein</title><description>The present invention relates to a method for expressing a target protein, and more specifically, to a signal peptide for secreting the target protein into periplasm, a nucleic acid encoding the same, an expression vector containing the nucleic acid, a recombinant microorganism into which the expression vector has been introduced, and a method for expressing the target protein using the recombinant microorganism. The present invention not only ensures that the target protein is well expressed in E. coli, but also improves secretion efficiency into the periplasmic. Accordingly, high-yield protein production is possible through the target protein expression method according to the present invention.
본 발명은 목적 단백질의 발현 방법에 관한 것으로, 더욱 상세하게는 목적 단백질을 주변 세포질(periplasmic)로 분비시키기 위한 신호 펩티드, 이를 코딩하는 핵산, 이 핵산을 포함하는 발현 벡터, 이 발현 벡터가 도입되어 있는 재조합 미생물, 및 이 재조합 미생물을 이용한 목적 단백질의 발현 방법에 관한 것이다. 본 발명은 목적 단백질이 대장균에서 잘 발현되도록 할 뿐만 아니라 주변 세포질(periplasmic)로의 분비 효율이 향상되도록 한다. 이에 본 발명에 따른 목적 단백질 발현 방법을 통해 높은 수율의 단백질 생산이 가능하다.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZFDxTS3JyE9RSMsvUnCtKChKLS7OzEtXCEksSk8tUQgoyi9JzczjYWBNS8wpTuWF0twMym6uIc4euqkF-fGpxQWJyal5qSXx3kFGBkbGBoYGRmYWRo7GxKkCAJXeJ3M</recordid><startdate>20230707</startdate><enddate>20230707</enddate><creator>LA WAN GEUN</creator><creator>RYU DONG KYUN</creator><creator>KIM MAN SU</creator><creator>LEE YOO WON</creator><scope>EVB</scope></search><sort><creationdate>20230707</creationdate><title>Method for Expressing Target Protein</title><author>LA WAN GEUN ; RYU DONG KYUN ; KIM MAN SU ; LEE YOO WON</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_KR20230102682A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; kor</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LA WAN GEUN</creatorcontrib><creatorcontrib>RYU DONG KYUN</creatorcontrib><creatorcontrib>KIM MAN SU</creatorcontrib><creatorcontrib>LEE YOO WON</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LA WAN GEUN</au><au>RYU DONG KYUN</au><au>KIM MAN SU</au><au>LEE YOO WON</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for Expressing Target Protein</title><date>2023-07-07</date><risdate>2023</risdate><abstract>The present invention relates to a method for expressing a target protein, and more specifically, to a signal peptide for secreting the target protein into periplasm, a nucleic acid encoding the same, an expression vector containing the nucleic acid, a recombinant microorganism into which the expression vector has been introduced, and a method for expressing the target protein using the recombinant microorganism. The present invention not only ensures that the target protein is well expressed in E. coli, but also improves secretion efficiency into the periplasmic. Accordingly, high-yield protein production is possible through the target protein expression method according to the present invention.
본 발명은 목적 단백질의 발현 방법에 관한 것으로, 더욱 상세하게는 목적 단백질을 주변 세포질(periplasmic)로 분비시키기 위한 신호 펩티드, 이를 코딩하는 핵산, 이 핵산을 포함하는 발현 벡터, 이 발현 벡터가 도입되어 있는 재조합 미생물, 및 이 재조합 미생물을 이용한 목적 단백질의 발현 방법에 관한 것이다. 본 발명은 목적 단백질이 대장균에서 잘 발현되도록 할 뿐만 아니라 주변 세포질(periplasmic)로의 분비 효율이 향상되도록 한다. 이에 본 발명에 따른 목적 단백질 발현 방법을 통해 높은 수율의 단백질 생산이 가능하다.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for Expressing Target Protein |
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