스테로이드의 C19-히드록실화를 위한 P450 BM3 모노옥시게나제 변이체
본 발명은 스테로이드 및 이의 유도체의 C19 히드록실화 또는 개선된 BM3 단백질 발현을 위한 신규 재조합 바실러스 메가테리움 (Bacillus megaterium) 사이토크롬 P450-모노옥시게나제 (P450-BM3) 변이체에 관한 것이다. 특히, 본 발명은 또한 에스트론 및 에스트라디올의 생산을 위해 P450-BM3 변이체를 사용하는 방법 및 과정에 관한 것이다. 본 발명은 또한 이들 P450-BM3 변이체의 발현을 위한 뉴클레오티드 서열, 구성체, 및 벡터에 관한 것이다. The present invention relates...
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| creator | WAGNER JAKOB LUDWIG MARCUS ZORN LUDWIG BERENDES FRANK COCO WAYNE RICHTER FLORIAN SKALDEN LILLY HELFRICH PETRA BURMEISTER JENS TRENNER SABINE BULUT DALIA KRETSCHMANN NILS PILLING JENS KENSCH OLIVER THEDE KAI LINNHOFF RUBEN |
| description | 본 발명은 스테로이드 및 이의 유도체의 C19 히드록실화 또는 개선된 BM3 단백질 발현을 위한 신규 재조합 바실러스 메가테리움 (Bacillus megaterium) 사이토크롬 P450-모노옥시게나제 (P450-BM3) 변이체에 관한 것이다. 특히, 본 발명은 또한 에스트론 및 에스트라디올의 생산을 위해 P450-BM3 변이체를 사용하는 방법 및 과정에 관한 것이다. 본 발명은 또한 이들 P450-BM3 변이체의 발현을 위한 뉴클레오티드 서열, 구성체, 및 벡터에 관한 것이다.
The present invention relates to novel recombinant Bacillus megaterium cytochrome P450-monooxygenase (P450-BM3) variants for the C19 hydroxylation of steroids and derivatives thereof. In particular, the present invention also relates to methods and processes using P450-BM3 variants for the production of estrone and estradiol. The invention further relates to nucleotide sequences, constructs and vectors for the expression of these P450-BM3 variants |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_KR20220136374A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>KR20220136374A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_KR20220136374A3</originalsourceid><addsrcrecordid>eNrjZIh807XkbWvP64Vz3szd8noykJyh4Gxoqft2XgeQ93rh3DfdS97OnPJ66R6FN3Na3k6doxBgYmqg4ORrrPB61YrXrTvezFj6pnvOq009r5tmvFkwR-H15gagSW82beFhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHeQUYGRkYGhsZmxuYmjsbEqQIAIBFQuQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>스테로이드의 C19-히드록실화를 위한 P450 BM3 모노옥시게나제 변이체</title><source>esp@cenet</source><creator>WAGNER JAKOB ; LUDWIG MARCUS ; ZORN LUDWIG ; BERENDES FRANK ; COCO WAYNE ; RICHTER FLORIAN ; SKALDEN LILLY ; HELFRICH PETRA ; BURMEISTER JENS ; TRENNER SABINE ; BULUT DALIA ; KRETSCHMANN NILS ; PILLING JENS ; KENSCH OLIVER ; THEDE KAI ; LINNHOFF RUBEN</creator><creatorcontrib>WAGNER JAKOB ; LUDWIG MARCUS ; ZORN LUDWIG ; BERENDES FRANK ; COCO WAYNE ; RICHTER FLORIAN ; SKALDEN LILLY ; HELFRICH PETRA ; BURMEISTER JENS ; TRENNER SABINE ; BULUT DALIA ; KRETSCHMANN NILS ; PILLING JENS ; KENSCH OLIVER ; THEDE KAI ; LINNHOFF RUBEN</creatorcontrib><description>본 발명은 스테로이드 및 이의 유도체의 C19 히드록실화 또는 개선된 BM3 단백질 발현을 위한 신규 재조합 바실러스 메가테리움 (Bacillus megaterium) 사이토크롬 P450-모노옥시게나제 (P450-BM3) 변이체에 관한 것이다. 특히, 본 발명은 또한 에스트론 및 에스트라디올의 생산을 위해 P450-BM3 변이체를 사용하는 방법 및 과정에 관한 것이다. 본 발명은 또한 이들 P450-BM3 변이체의 발현을 위한 뉴클레오티드 서열, 구성체, 및 벡터에 관한 것이다.
The present invention relates to novel recombinant Bacillus megaterium cytochrome P450-monooxygenase (P450-BM3) variants for the C19 hydroxylation of steroids and derivatives thereof. In particular, the present invention also relates to methods and processes using P450-BM3 variants for the production of estrone and estradiol. The invention further relates to nucleotide sequences, constructs and vectors for the expression of these P450-BM3 variants</description><language>kor</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20221007&DB=EPODOC&CC=KR&NR=20220136374A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20221007&DB=EPODOC&CC=KR&NR=20220136374A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WAGNER JAKOB</creatorcontrib><creatorcontrib>LUDWIG MARCUS</creatorcontrib><creatorcontrib>ZORN LUDWIG</creatorcontrib><creatorcontrib>BERENDES FRANK</creatorcontrib><creatorcontrib>COCO WAYNE</creatorcontrib><creatorcontrib>RICHTER FLORIAN</creatorcontrib><creatorcontrib>SKALDEN LILLY</creatorcontrib><creatorcontrib>HELFRICH PETRA</creatorcontrib><creatorcontrib>BURMEISTER JENS</creatorcontrib><creatorcontrib>TRENNER SABINE</creatorcontrib><creatorcontrib>BULUT DALIA</creatorcontrib><creatorcontrib>KRETSCHMANN NILS</creatorcontrib><creatorcontrib>PILLING JENS</creatorcontrib><creatorcontrib>KENSCH OLIVER</creatorcontrib><creatorcontrib>THEDE KAI</creatorcontrib><creatorcontrib>LINNHOFF RUBEN</creatorcontrib><title>스테로이드의 C19-히드록실화를 위한 P450 BM3 모노옥시게나제 변이체</title><description>본 발명은 스테로이드 및 이의 유도체의 C19 히드록실화 또는 개선된 BM3 단백질 발현을 위한 신규 재조합 바실러스 메가테리움 (Bacillus megaterium) 사이토크롬 P450-모노옥시게나제 (P450-BM3) 변이체에 관한 것이다. 특히, 본 발명은 또한 에스트론 및 에스트라디올의 생산을 위해 P450-BM3 변이체를 사용하는 방법 및 과정에 관한 것이다. 본 발명은 또한 이들 P450-BM3 변이체의 발현을 위한 뉴클레오티드 서열, 구성체, 및 벡터에 관한 것이다.
The present invention relates to novel recombinant Bacillus megaterium cytochrome P450-monooxygenase (P450-BM3) variants for the C19 hydroxylation of steroids and derivatives thereof. In particular, the present invention also relates to methods and processes using P450-BM3 variants for the production of estrone and estradiol. The invention further relates to nucleotide sequences, constructs and vectors for the expression of these P450-BM3 variants</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZIh807XkbWvP64Vz3szd8noykJyh4Gxoqft2XgeQ93rh3DfdS97OnPJ66R6FN3Na3k6doxBgYmqg4ORrrPB61YrXrTvezFj6pnvOq009r5tmvFkwR-H15gagSW82beFhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHeQUYGRkYGhsZmxuYmjsbEqQIAIBFQuQ</recordid><startdate>20221007</startdate><enddate>20221007</enddate><creator>WAGNER JAKOB</creator><creator>LUDWIG MARCUS</creator><creator>ZORN LUDWIG</creator><creator>BERENDES FRANK</creator><creator>COCO WAYNE</creator><creator>RICHTER FLORIAN</creator><creator>SKALDEN LILLY</creator><creator>HELFRICH PETRA</creator><creator>BURMEISTER JENS</creator><creator>TRENNER SABINE</creator><creator>BULUT DALIA</creator><creator>KRETSCHMANN NILS</creator><creator>PILLING JENS</creator><creator>KENSCH OLIVER</creator><creator>THEDE KAI</creator><creator>LINNHOFF RUBEN</creator><scope>EVB</scope></search><sort><creationdate>20221007</creationdate><title>스테로이드의 C19-히드록실화를 위한 P450 BM3 모노옥시게나제 변이체</title><author>WAGNER JAKOB ; LUDWIG MARCUS ; ZORN LUDWIG ; BERENDES FRANK ; COCO WAYNE ; RICHTER FLORIAN ; SKALDEN LILLY ; HELFRICH PETRA ; BURMEISTER JENS ; TRENNER SABINE ; BULUT DALIA ; KRETSCHMANN NILS ; PILLING JENS ; KENSCH OLIVER ; THEDE KAI ; LINNHOFF RUBEN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_KR20220136374A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>kor</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WAGNER JAKOB</creatorcontrib><creatorcontrib>LUDWIG MARCUS</creatorcontrib><creatorcontrib>ZORN LUDWIG</creatorcontrib><creatorcontrib>BERENDES FRANK</creatorcontrib><creatorcontrib>COCO WAYNE</creatorcontrib><creatorcontrib>RICHTER FLORIAN</creatorcontrib><creatorcontrib>SKALDEN LILLY</creatorcontrib><creatorcontrib>HELFRICH PETRA</creatorcontrib><creatorcontrib>BURMEISTER JENS</creatorcontrib><creatorcontrib>TRENNER SABINE</creatorcontrib><creatorcontrib>BULUT DALIA</creatorcontrib><creatorcontrib>KRETSCHMANN NILS</creatorcontrib><creatorcontrib>PILLING JENS</creatorcontrib><creatorcontrib>KENSCH OLIVER</creatorcontrib><creatorcontrib>THEDE KAI</creatorcontrib><creatorcontrib>LINNHOFF RUBEN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WAGNER JAKOB</au><au>LUDWIG MARCUS</au><au>ZORN LUDWIG</au><au>BERENDES FRANK</au><au>COCO WAYNE</au><au>RICHTER FLORIAN</au><au>SKALDEN LILLY</au><au>HELFRICH PETRA</au><au>BURMEISTER JENS</au><au>TRENNER SABINE</au><au>BULUT DALIA</au><au>KRETSCHMANN NILS</au><au>PILLING JENS</au><au>KENSCH OLIVER</au><au>THEDE KAI</au><au>LINNHOFF RUBEN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>스테로이드의 C19-히드록실화를 위한 P450 BM3 모노옥시게나제 변이체</title><date>2022-10-07</date><risdate>2022</risdate><abstract>본 발명은 스테로이드 및 이의 유도체의 C19 히드록실화 또는 개선된 BM3 단백질 발현을 위한 신규 재조합 바실러스 메가테리움 (Bacillus megaterium) 사이토크롬 P450-모노옥시게나제 (P450-BM3) 변이체에 관한 것이다. 특히, 본 발명은 또한 에스트론 및 에스트라디올의 생산을 위해 P450-BM3 변이체를 사용하는 방법 및 과정에 관한 것이다. 본 발명은 또한 이들 P450-BM3 변이체의 발현을 위한 뉴클레오티드 서열, 구성체, 및 벡터에 관한 것이다.
The present invention relates to novel recombinant Bacillus megaterium cytochrome P450-monooxygenase (P450-BM3) variants for the C19 hydroxylation of steroids and derivatives thereof. In particular, the present invention also relates to methods and processes using P450-BM3 variants for the production of estrone and estradiol. The invention further relates to nucleotide sequences, constructs and vectors for the expression of these P450-BM3 variants</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | 스테로이드의 C19-히드록실화를 위한 P450 BM3 모노옥시게나제 변이체 |
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