Extracellular vesicles comprising an antigen protein derived from coronavirus or a gene encoding the protein and uses thereof

The present invention relates to extracellular vesicles comprising a Coronavirus-derived antigen protein or a gene encoding the protein, and to a use thereof and, more specifically, to extracellular vesicles comprising a Coronavirus-derived antigen protein or a gene encoding the protein, or a vaccin...

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Bibliographische Detailangaben
Hauptverfasser:	LEE EUN HEE, CHUNG JI HWA, KWON KI HWAN
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
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Beschreibung
Zusammenfassung:	The present invention relates to extracellular vesicles comprising a Coronavirus-derived antigen protein or a gene encoding the protein, and to a use thereof and, more specifically, to extracellular vesicles comprising a Coronavirus-derived antigen protein or a gene encoding the protein, or a vaccine composition for preventing or treating Coronavirus infection comprising the same. In the present invention, by using activated immune cells loaded with an antigen protein or mRNA, a mouse was administered with extracellular vesicles derived therefrom and immunized, and as a result, excellent effects of generating antigen-specific neutralizing antibodies and inducing T cell responses were identified, and when the extracellular vesicles were stored at room temperature and refrigeration conditions after freeze-drying, excellent stability and an antigen generating effect were experimentally confirmed. From here, the extracellular vesicles according to the present invention, or a vaccine composition comprising the same, may serve as a platform applicable to various diseases that has stability and an excellent effect of inducing antigen-specific immune responses, and as such, can be expected to be advantageously utilized in the development of prophylactic or therapeutic vaccines for various diseases including infectious diseases, and in particular, Coronavirus infections. 본 발명은 코로나바이러스 유래 항원 단백질 또는 상기 단백질을 암호화하는 유전자를 포함하는 세포외소포체 및 그의 용도에 관한 것으로서, 보다 구체적으로는 코로나바이러스 유래 항원 단백질 또는 상기 단백질을 암호화하는 유전자를 포함하는 세포외소포체 또는 이를 포함하는 코로나바이러스 감염증 예방 또는 치료용 백신 조성물에 관한 것이다. 본 발명에서는 항원 단백질 또는 mRNA가 로딩된 활성화된 면역세포를 이용하여 이로부터 유래한 세포외소포체를 마우스에 투여해 면역화시킨 결과, 우수한 항원 특이적 중화항체 생성 및 T 세포 반응 유도 효과를 확인하였고, 동결건조 후 상온 및 냉장 조건에서 저장할 경우 우수한 안정성 및 항체 생성 효과를 실험적으로 확인하였다. 이로부터, 본 발명에 따른 세포외소포체 또는 이를 포함하는 백신 조성물은 우수한 항원 특이적 면역반응 유도 효과 및 안정성을 갖춘 다양한 질환에 적용 가능한 플랫폼으로써 감염성 질환, 특히 코로나바이러스 감염증을 포함한 다양한 질환의 예방 또는 치료용 백신 개발분야에서 유용하게 활용될 수 있을 것으로 기대된다.