PCR PCR PCR primer set and probe for detection of Gobiobotia naktongensis and real-time PCR method using the same
The present invention relates to a method for discriminating Gobiobotia nakdongensis from other freshwater fishes by amplifying a target sequence specific for Gobiobotia nakdongensis using a primer set comprising the oligonucleotide of SEQ ID NO: 3 and the oligonucleotide of SEQ ID NO: 4 which are P...
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| creator | KIM KEUN SIK YOON JOO DEOK KANG DONG WON |
| description | The present invention relates to a method for discriminating Gobiobotia nakdongensis from other freshwater fishes by amplifying a target sequence specific for Gobiobotia nakdongensis using a primer set comprising the oligonucleotide of SEQ ID NO: 3 and the oligonucleotide of SEQ ID NO: 4 which are PCR primers. In addition, the present invention relates to a method for rapidly detecting Gobiobotia nakdongensis with excellent specificity and sensitivity from environmental water through real time PCR using a mixture including a probe for detecting the oligonucleotide of SEQ ID NO: 3, the oligonucleotide of SEQ ID NO: 4 and oligonucleotides of SEQ ID NO: 5 derived from Gobiobotia nakdongensis.
본 발명은 PCR 프라이머인 서열번호 3의 올리고뉴클레오티드 및 서열번호 4의 올리고뉴클레오티드를 포함하는 프라이머 세트를 사용하여 흰수마자에 특이적인 표적 서열을 증폭시킴으로써 다른 담수어류와 흰수마자를 식별하는 방법에 관한 것이다. 또한 본 발명은 서열번호 3의 올리고뉴클레오티드, 서열번호 4의 올리고뉴클레오티드 및 서열번호 5의 흰수마자에서 유래한 올리고뉴클레오티드 검출용 프로브를 포함하는 혼합물을 사용하여 실시간 PCR을 통해 환경수로부터 흰수마자를 우수한 특이성과 민감도로 신속하게 검출할 수 있는 방법에 관한 것이다. |
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본 발명은 PCR 프라이머인 서열번호 3의 올리고뉴클레오티드 및 서열번호 4의 올리고뉴클레오티드를 포함하는 프라이머 세트를 사용하여 흰수마자에 특이적인 표적 서열을 증폭시킴으로써 다른 담수어류와 흰수마자를 식별하는 방법에 관한 것이다. 또한 본 발명은 서열번호 3의 올리고뉴클레오티드, 서열번호 4의 올리고뉴클레오티드 및 서열번호 5의 흰수마자에서 유래한 올리고뉴클레오티드 검출용 프로브를 포함하는 혼합물을 사용하여 실시간 PCR을 통해 환경수로부터 흰수마자를 우수한 특이성과 민감도로 신속하게 검출할 수 있는 방법에 관한 것이다.</description><language>eng ; kor</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210510&DB=EPODOC&CC=KR&NR=20210050754A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25569,76552</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210510&DB=EPODOC&CC=KR&NR=20210050754A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KIM KEUN SIK</creatorcontrib><creatorcontrib>YOON JOO DEOK</creatorcontrib><creatorcontrib>KANG DONG WON</creatorcontrib><title>PCR PCR PCR primer set and probe for detection of Gobiobotia naktongensis and real-time PCR method using the same</title><description>The present invention relates to a method for discriminating Gobiobotia nakdongensis from other freshwater fishes by amplifying a target sequence specific for Gobiobotia nakdongensis using a primer set comprising the oligonucleotide of SEQ ID NO: 3 and the oligonucleotide of SEQ ID NO: 4 which are PCR primers. In addition, the present invention relates to a method for rapidly detecting Gobiobotia nakdongensis with excellent specificity and sensitivity from environmental water through real time PCR using a mixture including a probe for detecting the oligonucleotide of SEQ ID NO: 3, the oligonucleotide of SEQ ID NO: 4 and oligonucleotides of SEQ ID NO: 5 derived from Gobiobotia nakdongensis.
본 발명은 PCR 프라이머인 서열번호 3의 올리고뉴클레오티드 및 서열번호 4의 올리고뉴클레오티드를 포함하는 프라이머 세트를 사용하여 흰수마자에 특이적인 표적 서열을 증폭시킴으로써 다른 담수어류와 흰수마자를 식별하는 방법에 관한 것이다. 또한 본 발명은 서열번호 3의 올리고뉴클레오티드, 서열번호 4의 올리고뉴클레오티드 및 서열번호 5의 흰수마자에서 유래한 올리고뉴클레오티드 검출용 프로브를 포함하는 혼합물을 사용하여 실시간 PCR을 통해 환경수로부터 흰수마자를 우수한 특이성과 민감도로 신속하게 검출할 수 있는 방법에 관한 것이다.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjD0LwkAQRNNYiPofFqwDZ1SsJfgBNhLswyWZJIfJbrxb_78haG8xDANv3jx63dOMfhm86-EpQMlyNU4pQLV4qqAo1QmT1HSRwkkh6iyxfapwAw4uTBcP28U6WiZfD22londw3JC2oGB7LKNZbbuA1bcX0fp8eqTXGIPkCIMtwdD8liUm2RizN4f97rj9j_oAMitCUQ</recordid><startdate>20210510</startdate><enddate>20210510</enddate><creator>KIM KEUN SIK</creator><creator>YOON JOO DEOK</creator><creator>KANG DONG WON</creator><scope>EVB</scope></search><sort><creationdate>20210510</creationdate><title>PCR PCR PCR primer set and probe for detection of Gobiobotia naktongensis and real-time PCR method using the same</title><author>KIM KEUN SIK ; YOON JOO DEOK ; KANG DONG WON</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_KR20210050754A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; kor</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KIM KEUN SIK</creatorcontrib><creatorcontrib>YOON JOO DEOK</creatorcontrib><creatorcontrib>KANG DONG WON</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KIM KEUN SIK</au><au>YOON JOO DEOK</au><au>KANG DONG WON</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PCR PCR PCR primer set and probe for detection of Gobiobotia naktongensis and real-time PCR method using the same</title><date>2021-05-10</date><risdate>2021</risdate><abstract>The present invention relates to a method for discriminating Gobiobotia nakdongensis from other freshwater fishes by amplifying a target sequence specific for Gobiobotia nakdongensis using a primer set comprising the oligonucleotide of SEQ ID NO: 3 and the oligonucleotide of SEQ ID NO: 4 which are PCR primers. In addition, the present invention relates to a method for rapidly detecting Gobiobotia nakdongensis with excellent specificity and sensitivity from environmental water through real time PCR using a mixture including a probe for detecting the oligonucleotide of SEQ ID NO: 3, the oligonucleotide of SEQ ID NO: 4 and oligonucleotides of SEQ ID NO: 5 derived from Gobiobotia nakdongensis.
본 발명은 PCR 프라이머인 서열번호 3의 올리고뉴클레오티드 및 서열번호 4의 올리고뉴클레오티드를 포함하는 프라이머 세트를 사용하여 흰수마자에 특이적인 표적 서열을 증폭시킴으로써 다른 담수어류와 흰수마자를 식별하는 방법에 관한 것이다. 또한 본 발명은 서열번호 3의 올리고뉴클레오티드, 서열번호 4의 올리고뉴클레오티드 및 서열번호 5의 흰수마자에서 유래한 올리고뉴클레오티드 검출용 프로브를 포함하는 혼합물을 사용하여 실시간 PCR을 통해 환경수로부터 흰수마자를 우수한 특이성과 민감도로 신속하게 검출할 수 있는 방법에 관한 것이다.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | PCR PCR PCR primer set and probe for detection of Gobiobotia naktongensis and real-time PCR method using the same |
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