METHOD OF DETECTING CIRCULATING TUMOR CELL BY USING FERROFLUID IN MICROFLUIDIC CHANNEL AND MICROFLUIDIC CHIP THEREFOR
The present invention relates to a method for detecting tumor cells from a blood sample containing tumor cells and normal cells. The method includes the steps of: providing a microfluidic chip including two micro-separation channels and two magnetic field-forming portions provided adjacently to each...
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| creator | KYE HYEON GI SHIN CHOONG SOO CHUNG BONG GEUN SONG HAN GYEOL LEE JONG MIN GONG YOUNG GUN SONG MIN GYU KIM TAE YEONG |
| description | The present invention relates to a method for detecting tumor cells from a blood sample containing tumor cells and normal cells. The method includes the steps of: providing a microfluidic chip including two micro-separation channels and two magnetic field-forming portions provided adjacently to each of the micro-separation channels; providing a viscoelastic solution including a blood sample and a magnetic fluid to the first micro-separation channel through an inlet; allowing the viscoelastic solution to pass through the surroundings of the first magnetic field-forming portion along the first micro-separation channel, thereby carrying out primary separation of the cells of the viscoelastic solution depending on particle sizes; allowing a part of the viscoelastic solution introduced to the second micro-separation channel through the surroundings of the second magnetic field-forming portion, thereby carrying out secondary separation of the tumor cells and normal cells of the viscoelastic solution; and collecting the tumor cells discharged from an outlet.
종양 세포와 정상 세포를 포함하는 혈액 샘플로부터 종양 세포를 검출하기 위한 종양 세포 검출방법은, 2개의 미세 분리 채널과 각 미세 분리 채널에 인접하게 제공되는 2개의 자기장 형성부를 포함하는 미세 유체칩을 제공하는 단계, 혈액 샘플과 자성 유체를 포함하는 점탄성 용액을 입구를 통해 제1 미세 분리 채널로 제공하는 단계, 제1 미세 분리 채널을 따라 점탄성 용액이 제1 자기장 형성부의 주변을 통과하도록 하여 점탄성 용액의 세포를 입자 크기에 따라 제1차로 분리하는 제1차 분리 단계, 제2 미세 분리 채널로 유입된 점탄성 용액의 일부가 제2 자기장 형성부의 주변을 통과하도록 하여 제2 미세 분리 채널을 통과하는 점탄성 용액의 종양 세포와 정상 세포를 제2차로 분리하는 제2 분리 단계 및 출구를 통해 배출되는 종양 세포를 수집하는 단계를 포함한다. |
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종양 세포와 정상 세포를 포함하는 혈액 샘플로부터 종양 세포를 검출하기 위한 종양 세포 검출방법은, 2개의 미세 분리 채널과 각 미세 분리 채널에 인접하게 제공되는 2개의 자기장 형성부를 포함하는 미세 유체칩을 제공하는 단계, 혈액 샘플과 자성 유체를 포함하는 점탄성 용액을 입구를 통해 제1 미세 분리 채널로 제공하는 단계, 제1 미세 분리 채널을 따라 점탄성 용액이 제1 자기장 형성부의 주변을 통과하도록 하여 점탄성 용액의 세포를 입자 크기에 따라 제1차로 분리하는 제1차 분리 단계, 제2 미세 분리 채널로 유입된 점탄성 용액의 일부가 제2 자기장 형성부의 주변을 통과하도록 하여 제2 미세 분리 채널을 통과하는 점탄성 용액의 종양 세포와 정상 세포를 제2차로 분리하는 제2 분리 단계 및 출구를 통해 배출되는 종양 세포를 수집하는 단계를 포함한다.</description><language>eng ; kor</language><subject>CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; PHYSICS ; TESTING ; TRANSPORTING</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200731&DB=EPODOC&CC=KR&NR=20200091538A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20200731&DB=EPODOC&CC=KR&NR=20200091538A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KYE HYEON GI</creatorcontrib><creatorcontrib>SHIN CHOONG SOO</creatorcontrib><creatorcontrib>CHUNG BONG GEUN</creatorcontrib><creatorcontrib>SONG HAN GYEOL</creatorcontrib><creatorcontrib>LEE JONG MIN</creatorcontrib><creatorcontrib>GONG YOUNG GUN</creatorcontrib><creatorcontrib>SONG MIN GYU</creatorcontrib><creatorcontrib>KIM TAE YEONG</creatorcontrib><title>METHOD OF DETECTING CIRCULATING TUMOR CELL BY USING FERROFLUID IN MICROFLUIDIC CHANNEL AND MICROFLUIDIC CHIP THEREFOR</title><description>The present invention relates to a method for detecting tumor cells from a blood sample containing tumor cells and normal cells. The method includes the steps of: providing a microfluidic chip including two micro-separation channels and two magnetic field-forming portions provided adjacently to each of the micro-separation channels; providing a viscoelastic solution including a blood sample and a magnetic fluid to the first micro-separation channel through an inlet; allowing the viscoelastic solution to pass through the surroundings of the first magnetic field-forming portion along the first micro-separation channel, thereby carrying out primary separation of the cells of the viscoelastic solution depending on particle sizes; allowing a part of the viscoelastic solution introduced to the second micro-separation channel through the surroundings of the second magnetic field-forming portion, thereby carrying out secondary separation of the tumor cells and normal cells of the viscoelastic solution; and collecting the tumor cells discharged from an outlet.
종양 세포와 정상 세포를 포함하는 혈액 샘플로부터 종양 세포를 검출하기 위한 종양 세포 검출방법은, 2개의 미세 분리 채널과 각 미세 분리 채널에 인접하게 제공되는 2개의 자기장 형성부를 포함하는 미세 유체칩을 제공하는 단계, 혈액 샘플과 자성 유체를 포함하는 점탄성 용액을 입구를 통해 제1 미세 분리 채널로 제공하는 단계, 제1 미세 분리 채널을 따라 점탄성 용액이 제1 자기장 형성부의 주변을 통과하도록 하여 점탄성 용액의 세포를 입자 크기에 따라 제1차로 분리하는 제1차 분리 단계, 제2 미세 분리 채널로 유입된 점탄성 용액의 일부가 제2 자기장 형성부의 주변을 통과하도록 하여 제2 미세 분리 채널을 통과하는 점탄성 용액의 종양 세포와 정상 세포를 제2차로 분리하는 제2 분리 단계 및 출구를 통해 배출되는 종양 세포를 수집하는 단계를 포함한다.</description><subject>CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PERFORMING OPERATIONS</subject><subject>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</subject><subject>PHYSICS</subject><subject>TESTING</subject><subject>TRANSPORTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNirEKwjAURbs4iPoPD5yF2CLoGF9eTDBN5DUZnEqROIkWqv8vSicnp3vPPXdavGqKJigIGhRFwmj9AdAyJie_PaY6MCA5B_szpOazaWIO2iWrwHqoLY5kEdBI78mB9OpX2BNEQ0w68LyYXLvbkBdjzoqlpohmlftHm4e-u-R7frZHLkUphNitN9VWVv-93reEOdo</recordid><startdate>20200731</startdate><enddate>20200731</enddate><creator>KYE HYEON GI</creator><creator>SHIN CHOONG SOO</creator><creator>CHUNG BONG GEUN</creator><creator>SONG HAN GYEOL</creator><creator>LEE JONG MIN</creator><creator>GONG YOUNG GUN</creator><creator>SONG MIN GYU</creator><creator>KIM TAE YEONG</creator><scope>EVB</scope></search><sort><creationdate>20200731</creationdate><title>METHOD OF DETECTING CIRCULATING TUMOR CELL BY USING FERROFLUID IN MICROFLUIDIC CHANNEL AND MICROFLUIDIC CHIP THEREFOR</title><author>KYE HYEON GI ; SHIN CHOONG SOO ; CHUNG BONG GEUN ; SONG HAN GYEOL ; LEE JONG MIN ; GONG YOUNG GUN ; SONG MIN GYU ; KIM TAE YEONG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_KR20200091538A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; kor</language><creationdate>2020</creationdate><topic>CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PERFORMING OPERATIONS</topic><topic>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</topic><topic>PHYSICS</topic><topic>TESTING</topic><topic>TRANSPORTING</topic><toplevel>online_resources</toplevel><creatorcontrib>KYE HYEON GI</creatorcontrib><creatorcontrib>SHIN CHOONG SOO</creatorcontrib><creatorcontrib>CHUNG BONG GEUN</creatorcontrib><creatorcontrib>SONG HAN GYEOL</creatorcontrib><creatorcontrib>LEE JONG MIN</creatorcontrib><creatorcontrib>GONG YOUNG GUN</creatorcontrib><creatorcontrib>SONG MIN GYU</creatorcontrib><creatorcontrib>KIM TAE YEONG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KYE HYEON GI</au><au>SHIN CHOONG SOO</au><au>CHUNG BONG GEUN</au><au>SONG HAN GYEOL</au><au>LEE JONG MIN</au><au>GONG YOUNG GUN</au><au>SONG MIN GYU</au><au>KIM TAE YEONG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD OF DETECTING CIRCULATING TUMOR CELL BY USING FERROFLUID IN MICROFLUIDIC CHANNEL AND MICROFLUIDIC CHIP THEREFOR</title><date>2020-07-31</date><risdate>2020</risdate><abstract>The present invention relates to a method for detecting tumor cells from a blood sample containing tumor cells and normal cells. The method includes the steps of: providing a microfluidic chip including two micro-separation channels and two magnetic field-forming portions provided adjacently to each of the micro-separation channels; providing a viscoelastic solution including a blood sample and a magnetic fluid to the first micro-separation channel through an inlet; allowing the viscoelastic solution to pass through the surroundings of the first magnetic field-forming portion along the first micro-separation channel, thereby carrying out primary separation of the cells of the viscoelastic solution depending on particle sizes; allowing a part of the viscoelastic solution introduced to the second micro-separation channel through the surroundings of the second magnetic field-forming portion, thereby carrying out secondary separation of the tumor cells and normal cells of the viscoelastic solution; and collecting the tumor cells discharged from an outlet.
종양 세포와 정상 세포를 포함하는 혈액 샘플로부터 종양 세포를 검출하기 위한 종양 세포 검출방법은, 2개의 미세 분리 채널과 각 미세 분리 채널에 인접하게 제공되는 2개의 자기장 형성부를 포함하는 미세 유체칩을 제공하는 단계, 혈액 샘플과 자성 유체를 포함하는 점탄성 용액을 입구를 통해 제1 미세 분리 채널로 제공하는 단계, 제1 미세 분리 채널을 따라 점탄성 용액이 제1 자기장 형성부의 주변을 통과하도록 하여 점탄성 용액의 세포를 입자 크기에 따라 제1차로 분리하는 제1차 분리 단계, 제2 미세 분리 채널로 유입된 점탄성 용액의 일부가 제2 자기장 형성부의 주변을 통과하도록 하여 제2 미세 분리 채널을 통과하는 점탄성 용액의 종양 세포와 정상 세포를 제2차로 분리하는 제2 분리 단계 및 출구를 통해 배출되는 종양 세포를 수집하는 단계를 포함한다.</abstract><oa>free_for_read</oa></addata></record> |
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| title | METHOD OF DETECTING CIRCULATING TUMOR CELL BY USING FERROFLUID IN MICROFLUIDIC CHANNEL AND MICROFLUIDIC CHIP THEREFOR |
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