레바우디오시드의 고효율 생산을 위한 UDP 의존성 글리코실트랜스퍼라제
본 명세서에서 숙주 세포에서 스테비올 글리코시드의 개선된 생산을 위한 조성물 및 방법을 제공한다. 일부 실시태양에서, 상기 숙주 세포를 세타리아 이탈리카 UDP-글리코실트랜스퍼라제 40087 또는 그의 변형 UDP-글리코실트랜스퍼라제를 암호화하는 이종 뉴클레오티드 서열을 포함하도록 유전자 변형시킨다. 일부 실시태양에서, 상기 숙주 세포를 UDP-글리코실트랜스퍼라제 sr.UGT_9252778, Bd_UGT10850, 및/또는 Ob_UGT91B1_유사를 암호화하는 이종 뉴클레오티드 서열을 포함하도록 유전자 변형시킨다. 일부 실시태양에서...
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| creator | LEAVELL MICHAEL JACKSON SHAINA KHANKHOJE ADITI PLATT DARREN ZHAO LISHAN GARCIA DE GONZALO CHANTAL WICHMANN GALE LI WENZONG MAHATDEJKUL MEADOWS TINA |
| description | 본 명세서에서 숙주 세포에서 스테비올 글리코시드의 개선된 생산을 위한 조성물 및 방법을 제공한다. 일부 실시태양에서, 상기 숙주 세포를 세타리아 이탈리카 UDP-글리코실트랜스퍼라제 40087 또는 그의 변형 UDP-글리코실트랜스퍼라제를 암호화하는 이종 뉴클레오티드 서열을 포함하도록 유전자 변형시킨다. 일부 실시태양에서, 상기 숙주 세포를 UDP-글리코실트랜스퍼라제 sr.UGT_9252778, Bd_UGT10850, 및/또는 Ob_UGT91B1_유사를 암호화하는 이종 뉴클레오티드 서열을 포함하도록 유전자 변형시킨다. 일부 실시태양에서, 상기 숙주 세포는 상기 숙주 세포에서 스테비올 글리코시드를 생산할 수 있는 경로의 추가의 효소들을 암호화하는 하나 이상의 이종 뉴클레오티드 서열을 추가로 포함한다. 본 명세서에 기재된 조성물 및 방법은 비제한적으로 레바우디오시드 D 및 레바우디오시드 M을 포함한 스테비올 글리코시드의 이종 생산에 효율적인 경로를 제공한다.
Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M. |
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| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_KR20190038612A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>KR20190038612A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_KR20190038612A3</originalsourceid><addsrcrecordid>eNrjZEh4vaDj9YYpb2ZteD1lypsZS950z3k9ec6buTMUXm1e8HbWijdzVii8aZ77pmnDm7ktCm_mtLydOkch1CVAAajkzcItb1o2Krza0fB62Zo3e6e86V7ytmvH63lz3nQtedu75_XcPW8WzOFhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHeQUYGhpYGBsYWZoZGjsbEqQIABWtaFA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>레바우디오시드의 고효율 생산을 위한 UDP 의존성 글리코실트랜스퍼라제</title><source>esp@cenet</source><creator>LEAVELL MICHAEL ; JACKSON SHAINA ; KHANKHOJE ADITI ; PLATT DARREN ; ZHAO LISHAN ; GARCIA DE GONZALO CHANTAL ; WICHMANN GALE ; LI WENZONG ; MAHATDEJKUL MEADOWS TINA</creator><creatorcontrib>LEAVELL MICHAEL ; JACKSON SHAINA ; KHANKHOJE ADITI ; PLATT DARREN ; ZHAO LISHAN ; GARCIA DE GONZALO CHANTAL ; WICHMANN GALE ; LI WENZONG ; MAHATDEJKUL MEADOWS TINA</creatorcontrib><description>본 명세서에서 숙주 세포에서 스테비올 글리코시드의 개선된 생산을 위한 조성물 및 방법을 제공한다. 일부 실시태양에서, 상기 숙주 세포를 세타리아 이탈리카 UDP-글리코실트랜스퍼라제 40087 또는 그의 변형 UDP-글리코실트랜스퍼라제를 암호화하는 이종 뉴클레오티드 서열을 포함하도록 유전자 변형시킨다. 일부 실시태양에서, 상기 숙주 세포를 UDP-글리코실트랜스퍼라제 sr.UGT_9252778, Bd_UGT10850, 및/또는 Ob_UGT91B1_유사를 암호화하는 이종 뉴클레오티드 서열을 포함하도록 유전자 변형시킨다. 일부 실시태양에서, 상기 숙주 세포는 상기 숙주 세포에서 스테비올 글리코시드를 생산할 수 있는 경로의 추가의 효소들을 암호화하는 하나 이상의 이종 뉴클레오티드 서열을 추가로 포함한다. 본 명세서에 기재된 조성물 및 방법은 비제한적으로 레바우디오시드 D 및 레바우디오시드 M을 포함한 스테비올 글리코시드의 이종 생산에 효율적인 경로를 제공한다.
Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.</description><language>kor</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; FOODS OR FOODSTUFFS ; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J ; HUMAN NECESSITIES ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT ; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190408&DB=EPODOC&CC=KR&NR=20190038612A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25569,76552</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190408&DB=EPODOC&CC=KR&NR=20190038612A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LEAVELL MICHAEL</creatorcontrib><creatorcontrib>JACKSON SHAINA</creatorcontrib><creatorcontrib>KHANKHOJE ADITI</creatorcontrib><creatorcontrib>PLATT DARREN</creatorcontrib><creatorcontrib>ZHAO LISHAN</creatorcontrib><creatorcontrib>GARCIA DE GONZALO CHANTAL</creatorcontrib><creatorcontrib>WICHMANN GALE</creatorcontrib><creatorcontrib>LI WENZONG</creatorcontrib><creatorcontrib>MAHATDEJKUL MEADOWS TINA</creatorcontrib><title>레바우디오시드의 고효율 생산을 위한 UDP 의존성 글리코실트랜스퍼라제</title><description>본 명세서에서 숙주 세포에서 스테비올 글리코시드의 개선된 생산을 위한 조성물 및 방법을 제공한다. 일부 실시태양에서, 상기 숙주 세포를 세타리아 이탈리카 UDP-글리코실트랜스퍼라제 40087 또는 그의 변형 UDP-글리코실트랜스퍼라제를 암호화하는 이종 뉴클레오티드 서열을 포함하도록 유전자 변형시킨다. 일부 실시태양에서, 상기 숙주 세포를 UDP-글리코실트랜스퍼라제 sr.UGT_9252778, Bd_UGT10850, 및/또는 Ob_UGT91B1_유사를 암호화하는 이종 뉴클레오티드 서열을 포함하도록 유전자 변형시킨다. 일부 실시태양에서, 상기 숙주 세포는 상기 숙주 세포에서 스테비올 글리코시드를 생산할 수 있는 경로의 추가의 효소들을 암호화하는 하나 이상의 이종 뉴클레오티드 서열을 추가로 포함한다. 본 명세서에 기재된 조성물 및 방법은 비제한적으로 레바우디오시드 D 및 레바우디오시드 M을 포함한 스테비올 글리코시드의 이종 생산에 효율적인 경로를 제공한다.
Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>FOODS OR FOODSTUFFS</subject><subject>FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J</subject><subject>HUMAN NECESSITIES</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT</subject><subject>THEIR TREATMENT, NOT COVERED BY OTHER CLASSES</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZEh4vaDj9YYpb2ZteD1lypsZS950z3k9ec6buTMUXm1e8HbWijdzVii8aZ77pmnDm7ktCm_mtLydOkch1CVAAajkzcItb1o2Krza0fB62Zo3e6e86V7ytmvH63lz3nQtedu75_XcPW8WzOFhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHeQUYGhpYGBsYWZoZGjsbEqQIABWtaFA</recordid><startdate>20190408</startdate><enddate>20190408</enddate><creator>LEAVELL MICHAEL</creator><creator>JACKSON SHAINA</creator><creator>KHANKHOJE ADITI</creator><creator>PLATT DARREN</creator><creator>ZHAO LISHAN</creator><creator>GARCIA DE GONZALO CHANTAL</creator><creator>WICHMANN GALE</creator><creator>LI WENZONG</creator><creator>MAHATDEJKUL MEADOWS TINA</creator><scope>EVB</scope></search><sort><creationdate>20190408</creationdate><title>레바우디오시드의 고효율 생산을 위한 UDP 의존성 글리코실트랜스퍼라제</title><author>LEAVELL MICHAEL ; JACKSON SHAINA ; KHANKHOJE ADITI ; PLATT DARREN ; ZHAO LISHAN ; GARCIA DE GONZALO CHANTAL ; WICHMANN GALE ; LI WENZONG ; MAHATDEJKUL MEADOWS TINA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_KR20190038612A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>kor</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>FOODS OR FOODSTUFFS</topic><topic>FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J</topic><topic>HUMAN NECESSITIES</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT</topic><topic>THEIR TREATMENT, NOT COVERED BY OTHER CLASSES</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LEAVELL MICHAEL</creatorcontrib><creatorcontrib>JACKSON SHAINA</creatorcontrib><creatorcontrib>KHANKHOJE ADITI</creatorcontrib><creatorcontrib>PLATT DARREN</creatorcontrib><creatorcontrib>ZHAO LISHAN</creatorcontrib><creatorcontrib>GARCIA DE GONZALO CHANTAL</creatorcontrib><creatorcontrib>WICHMANN GALE</creatorcontrib><creatorcontrib>LI WENZONG</creatorcontrib><creatorcontrib>MAHATDEJKUL MEADOWS TINA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LEAVELL MICHAEL</au><au>JACKSON SHAINA</au><au>KHANKHOJE ADITI</au><au>PLATT DARREN</au><au>ZHAO LISHAN</au><au>GARCIA DE GONZALO CHANTAL</au><au>WICHMANN GALE</au><au>LI WENZONG</au><au>MAHATDEJKUL MEADOWS TINA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>레바우디오시드의 고효율 생산을 위한 UDP 의존성 글리코실트랜스퍼라제</title><date>2019-04-08</date><risdate>2019</risdate><abstract>본 명세서에서 숙주 세포에서 스테비올 글리코시드의 개선된 생산을 위한 조성물 및 방법을 제공한다. 일부 실시태양에서, 상기 숙주 세포를 세타리아 이탈리카 UDP-글리코실트랜스퍼라제 40087 또는 그의 변형 UDP-글리코실트랜스퍼라제를 암호화하는 이종 뉴클레오티드 서열을 포함하도록 유전자 변형시킨다. 일부 실시태양에서, 상기 숙주 세포를 UDP-글리코실트랜스퍼라제 sr.UGT_9252778, Bd_UGT10850, 및/또는 Ob_UGT91B1_유사를 암호화하는 이종 뉴클레오티드 서열을 포함하도록 유전자 변형시킨다. 일부 실시태양에서, 상기 숙주 세포는 상기 숙주 세포에서 스테비올 글리코시드를 생산할 수 있는 경로의 추가의 효소들을 암호화하는 하나 이상의 이종 뉴클레오티드 서열을 추가로 포함한다. 본 명세서에 기재된 조성물 및 방법은 비제한적으로 레바우디오시드 D 및 레바우디오시드 M을 포함한 스테비올 글리코시드의 이종 생산에 효율적인 경로를 제공한다.
Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a Setaria italica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_UGT10850, and/or Ob_UGT91B1_like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.</abstract><oa>free_for_read</oa></addata></record> |
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| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE |
| title | 레바우디오시드의 고효율 생산을 위한 UDP 의존성 글리코실트랜스퍼라제 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-16T10%3A23%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=LEAVELL%20MICHAEL&rft.date=2019-04-08&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EKR20190038612A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |