N- Method for preparing recombinant glycoproteins with improved N-glycan antennary structure by inhibiting biosynthesis of polylactosamine
In the present invention, it was confirmed that polylactosamine was reduced in recombinant human-derived erythropoietin produced by inhibiting (knocking down) the expression of the β3gnt2 gene, which is involved in polylactosamine biosynthesis, or from a cell line with the knocked out β3gnt2 gene, a...
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| creator | KIM, JUNG HOE KWAK, CHAN YEONG LEE, CHUNG GEUN |
| description | In the present invention, it was confirmed that polylactosamine was reduced in recombinant human-derived erythropoietin produced by inhibiting (knocking down) the expression of the β3gnt2 gene, which is involved in polylactosamine biosynthesis, or from a cell line with the knocked out β3gnt2 gene, and thus triple and/or quadruple antenna structures of N-glycan increased, leading to positive roles in increasing the half-life of glycoprotein and the efficacy of glycoprotein. Therefore, it was confirmed that a recombinant glycoprotein with an increased half-life can be produced through the method of the present invention.
본 발명은 폴리락토사민의 생합성에 관여하는 β3gnt2 유전자의 발현을 억제(녹다운)하거나 β3gnt2 유전자를 녹아웃시킨 세포주로부터 생산된 재조합 인간 유래 에리스로포이에틴에서 폴리락토사민의 감소를 확인, 그에 따른 N-글리칸의 삼중 및/또는 사중 안테나 구조 증가를 확인함으로써 당단백질의 반감기 증가 및 당단백질 효능에 긍정적인 역할이 가능함을 확인한 바, 본 발명의 방법을 통해 반감기를 증가시킨 재조합 당단백질의 제조가 가능함을 확인하였다. |
| format | Patent |
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본 발명은 폴리락토사민의 생합성에 관여하는 β3gnt2 유전자의 발현을 억제(녹다운)하거나 β3gnt2 유전자를 녹아웃시킨 세포주로부터 생산된 재조합 인간 유래 에리스로포이에틴에서 폴리락토사민의 감소를 확인, 그에 따른 N-글리칸의 삼중 및/또는 사중 안테나 구조 증가를 확인함으로써 당단백질의 반감기 증가 및 당단백질 효능에 긍정적인 역할이 가능함을 확인한 바, 본 발명의 방법을 통해 반감기를 증가시킨 재조합 당단백질의 제조가 가능함을 확인하였다.</description><language>eng ; kor</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180220&DB=EPODOC&CC=KR&NR=20180016952A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20180220&DB=EPODOC&CC=KR&NR=20180016952A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KIM, JUNG HOE</creatorcontrib><creatorcontrib>KWAK, CHAN YEONG</creatorcontrib><creatorcontrib>LEE, CHUNG GEUN</creatorcontrib><title>N- Method for preparing recombinant glycoproteins with improved N-glycan antennary structure by inhibiting biosynthesis of polylactosamine</title><description>In the present invention, it was confirmed that polylactosamine was reduced in recombinant human-derived erythropoietin produced by inhibiting (knocking down) the expression of the β3gnt2 gene, which is involved in polylactosamine biosynthesis, or from a cell line with the knocked out β3gnt2 gene, and thus triple and/or quadruple antenna structures of N-glycan increased, leading to positive roles in increasing the half-life of glycoprotein and the efficacy of glycoprotein. Therefore, it was confirmed that a recombinant glycoprotein with an increased half-life can be produced through the method of the present invention.
본 발명은 폴리락토사민의 생합성에 관여하는 β3gnt2 유전자의 발현을 억제(녹다운)하거나 β3gnt2 유전자를 녹아웃시킨 세포주로부터 생산된 재조합 인간 유래 에리스로포이에틴에서 폴리락토사민의 감소를 확인, 그에 따른 N-글리칸의 삼중 및/또는 사중 안테나 구조 증가를 확인함으로써 당단백질의 반감기 증가 및 당단백질 효능에 긍정적인 역할이 가능함을 확인한 바, 본 발명의 방법을 통해 반감기를 증가시킨 재조합 당단백질의 제조가 가능함을 확인하였다.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2018</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjDEKwkAQRdNYiHqHAetAVBQtRRRBTCH2YbOZmIFkZtkdlb2CpzYBD2D1ebzHHyefPIUraiMV1OLBeXTGEz_Ao5WuJDas8GijFedFkTjAm7QB6np-YQV5OljD0IfIbHyEoP5p9ekRygjEDZWkw2VJEiJrg4ECSA1O2tgaqxJMR4zTZFSbNuDst5NkfjreD-cUnRQYnLHIqMXltswW2yxbbHbr5X71X_UFaTlPaQ</recordid><startdate>20180220</startdate><enddate>20180220</enddate><creator>KIM, JUNG HOE</creator><creator>KWAK, CHAN YEONG</creator><creator>LEE, CHUNG GEUN</creator><scope>EVB</scope></search><sort><creationdate>20180220</creationdate><title>N- Method for preparing recombinant glycoproteins with improved N-glycan antennary structure by inhibiting biosynthesis of polylactosamine</title><author>KIM, JUNG HOE ; KWAK, CHAN YEONG ; LEE, CHUNG GEUN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_KR20180016952A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; kor</language><creationdate>2018</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KIM, JUNG HOE</creatorcontrib><creatorcontrib>KWAK, CHAN YEONG</creatorcontrib><creatorcontrib>LEE, CHUNG GEUN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KIM, JUNG HOE</au><au>KWAK, CHAN YEONG</au><au>LEE, CHUNG GEUN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>N- Method for preparing recombinant glycoproteins with improved N-glycan antennary structure by inhibiting biosynthesis of polylactosamine</title><date>2018-02-20</date><risdate>2018</risdate><abstract>In the present invention, it was confirmed that polylactosamine was reduced in recombinant human-derived erythropoietin produced by inhibiting (knocking down) the expression of the β3gnt2 gene, which is involved in polylactosamine biosynthesis, or from a cell line with the knocked out β3gnt2 gene, and thus triple and/or quadruple antenna structures of N-glycan increased, leading to positive roles in increasing the half-life of glycoprotein and the efficacy of glycoprotein. Therefore, it was confirmed that a recombinant glycoprotein with an increased half-life can be produced through the method of the present invention.
본 발명은 폴리락토사민의 생합성에 관여하는 β3gnt2 유전자의 발현을 억제(녹다운)하거나 β3gnt2 유전자를 녹아웃시킨 세포주로부터 생산된 재조합 인간 유래 에리스로포이에틴에서 폴리락토사민의 감소를 확인, 그에 따른 N-글리칸의 삼중 및/또는 사중 안테나 구조 증가를 확인함으로써 당단백질의 반감기 증가 및 당단백질 효능에 긍정적인 역할이 가능함을 확인한 바, 본 발명의 방법을 통해 반감기를 증가시킨 재조합 당단백질의 제조가 가능함을 확인하였다.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | N- Method for preparing recombinant glycoproteins with improved N-glycan antennary structure by inhibiting biosynthesis of polylactosamine |
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