MASS PRODUCTION METHOD OF KERATINASE AND USE THEREOF

PURPOSE: A massive production of keratinase derived from Bacillus subtilis is provided to economically obtain keratinse and remove keratin from the skin. CONSTITUTION: A massive production of keratinase comprises: a step of preparing pH 7.0 of medium containing 1.0g/L of glucose, 7.0g/L of K2HPO4, 2...

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Bibliographische Detailangaben
Hauptverfasser:	PARK, SUNG MIN, CHO, SUNG BOK, KIM, HYUN SOO, JUNG, HYUCK JUN, KIM, MIN HEE, KO, HEE SUN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE: A massive production of keratinase derived from Bacillus subtilis is provided to economically obtain keratinse and remove keratin from the skin. CONSTITUTION: A massive production of keratinase comprises: a step of preparing pH 7.0 of medium containing 1.0g/L of glucose, 7.0g/L of K2HPO4, 2.0g/L of KH2PO4, 12g/L of soybean meal in distilled water; a step of adding 2 weight% of preculture of Bacillus subtilis SMMJ-2(KACC 91390P); and a step of culturing the medium at 30°C, 200rpm, and 2L/min of air for five days/ A cosmetic composition for removing keratin contains the keratinase.