EXTRACTION AND PURIFICATION METHOD OF PORPHYRAN WITH HYPERLIPIDEMIA TREATING ACTIVITY FROM PORPHYRA YEZOENSIS

PURPOSE: A method for extracting and purifying porphyran (treating hyperlipidemia) from porphyra yezoensis(laver) is provided, to improve the production yield and not to use toxic cetylpyridinium chloride. CONSTITUTION: The method comprises the steps of cutting laver finely, adding the laver into 0.1N HCl solution of 30-50 volume times, stirring the solution at 50-60 deg.C to obtain a porphyran extract, filtering the obtained porphyran extract, condensing the filtered one to make the volume be half; controlling the pH to be 6.0 by using a NaOH solution, adding 0.5-5 % of a proteolytic enzyme which is active in acidic condition to the condensed one and reacting the mixture at 50-60 deg.C to degrade the protein bonded to the extract, heating the mixture at 100 deg.C for 10-30 min to deactivate the enzyme, filtering the mixture, neutralizing it with a NaOH solution to make pH be 7.0, adding 3 times volume of ethyl alcohol to the condensed extract to fix it, centrifuging the fixed one, washing the centrifuged one to remove ethyl alcohol, drying it and grinding the dried one. Preferably the proteolytic enzyme is flavourzyme.