Use of plasmid containing luxCDABE and recombinant E. coli SB01 which was transformed by this plasmid

PURPOSE: A recombinant plasmid containing luxCDABE, E. coli SB01 (KFCC 11176) transformed therewith, and the use thereof are provided, therefore the stability of the recombinant plasmid in E. coli can be increased to use it for the detection of toxicity. CONSTITUTION: The recombinant plasmid pBS1 co...

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Hauptverfasser: SONG, BANG HO, BAE, HUI GYEONG, SHIN, PYEONG GYUN, OH, HYEON JU
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BAE, HUI GYEONG
SHIN, PYEONG GYUN
OH, HYEON JU
description PURPOSE: A recombinant plasmid containing luxCDABE, E. coli SB01 (KFCC 11176) transformed therewith, and the use thereof are provided, therefore the stability of the recombinant plasmid in E. coli can be increased to use it for the detection of toxicity. CONSTITUTION: The recombinant plasmid pBS1 contains a vector pKLJ12 containing luxCDABE derived from Photorhabdus luminescens. E. coli SB01 KFCC 11176 is produced by transforming E. coli DH5alpha with the recombinant plasmid pBS1. A method for detecting toxicity in a sample solution comprises culturing the transformed microorganism E. coli SB01 KFCC 11176 in the sample solution in a continuous fermenter, and analyzing the luminescence of the sample solution. 본 발명은 발광유전자 luxCDABE를 포함하는 재조합 플라스미드, 이 플라스미드에 의해 형질전환된 대장균 SB01 및 이의 용도에 관한 것으로서, 더욱 상세하게는 육지발광미생물 포토하버드스 루미네슨스()의 발광유전자(luxCDABE)가 삽입된 미니(mini)-F pKLJ12를 포함하는 재조합 플라스미드 pBS1, 이 플라스미드에 의해 형질전환된 대장균 SB01(KFCC 11176)을 제공하며, 이렇게 형질전환된 대장균 SB01이 600 세대(25 일)까지 항생제가 첨가되지 않은 배지상에서 안정된 플라스미드 유지를 보여 플라스미드의 안정성과 발광유전자를 이용해 독성탐지용 연속배양 장치에서의 미생물원으로 이용이 가능하고, 시판중인 발광미생물 킷(kit)의 수입 대체로 인한 경제적인 효과도 나타내는 발광유전자 luxCDABE를 포함하는 재조합 플라스미드, 이 플라스미드에 의해 형질 전환된 대장균 SB01 및 이의 용도에 관한 것이다.
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CONSTITUTION: The recombinant plasmid pBS1 contains a vector pKLJ12 containing luxCDABE derived from Photorhabdus luminescens. E. coli SB01 KFCC 11176 is produced by transforming E. coli DH5alpha with the recombinant plasmid pBS1. A method for detecting toxicity in a sample solution comprises culturing the transformed microorganism E. coli SB01 KFCC 11176 in the sample solution in a continuous fermenter, and analyzing the luminescence of the sample solution. 본 발명은 발광유전자 luxCDABE를 포함하는 재조합 플라스미드, 이 플라스미드에 의해 형질전환된 대장균 SB01 및 이의 용도에 관한 것으로서, 더욱 상세하게는 육지발광미생물 포토하버드스 루미네슨스()의 발광유전자(luxCDABE)가 삽입된 미니(mini)-F pKLJ12를 포함하는 재조합 플라스미드 pBS1, 이 플라스미드에 의해 형질전환된 대장균 SB01(KFCC 11176)을 제공하며, 이렇게 형질전환된 대장균 SB01이 600 세대(25 일)까지 항생제가 첨가되지 않은 배지상에서 안정된 플라스미드 유지를 보여 플라스미드의 안정성과 발광유전자를 이용해 독성탐지용 연속배양 장치에서의 미생물원으로 이용이 가능하고, 시판중인 발광미생물 킷(kit)의 수입 대체로 인한 경제적인 효과도 나타내는 발광유전자 luxCDABE를 포함하는 재조합 플라스미드, 이 플라스미드에 의해 형질 전환된 대장균 SB01 및 이의 용도에 관한 것이다.</description><edition>7</edition><language>eng ; kor</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2002</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20020603&amp;DB=EPODOC&amp;CC=KR&amp;NR=20020041603A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20020603&amp;DB=EPODOC&amp;CC=KR&amp;NR=20020041603A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SONG, BANG HO</creatorcontrib><creatorcontrib>BAE, HUI GYEONG</creatorcontrib><creatorcontrib>SHIN, PYEONG GYUN</creatorcontrib><creatorcontrib>OH, HYEON JU</creatorcontrib><title>Use of plasmid containing luxCDABE and recombinant E. coli SB01 which was transformed by this plasmid</title><description>PURPOSE: A recombinant plasmid containing luxCDABE, E. coli SB01 (KFCC 11176) transformed therewith, and the use thereof are provided, therefore the stability of the recombinant plasmid in E. coli can be increased to use it for the detection of toxicity. 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A method for detecting toxicity in a sample solution comprises culturing the transformed microorganism E. coli SB01 KFCC 11176 in the sample solution in a continuous fermenter, and analyzing the luminescence of the sample solution. 본 발명은 발광유전자 luxCDABE를 포함하는 재조합 플라스미드, 이 플라스미드에 의해 형질전환된 대장균 SB01 및 이의 용도에 관한 것으로서, 더욱 상세하게는 육지발광미생물 포토하버드스 루미네슨스()의 발광유전자(luxCDABE)가 삽입된 미니(mini)-F pKLJ12를 포함하는 재조합 플라스미드 pBS1, 이 플라스미드에 의해 형질전환된 대장균 SB01(KFCC 11176)을 제공하며, 이렇게 형질전환된 대장균 SB01이 600 세대(25 일)까지 항생제가 첨가되지 않은 배지상에서 안정된 플라스미드 유지를 보여 플라스미드의 안정성과 발광유전자를 이용해 독성탐지용 연속배양 장치에서의 미생물원으로 이용이 가능하고, 시판중인 발광미생물 킷(kit)의 수입 대체로 인한 경제적인 효과도 나타내는 발광유전자 luxCDABE를 포함하는 재조합 플라스미드, 이 플라스미드에 의해 형질 전환된 대장균 SB01 및 이의 용도에 관한 것이다.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2002</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjLsKwkAQANNYiPoPC9bKxYh9EiOCnY86rJeNWbjshexJ9O9Nob0wMM0w04huSuBr6BxqyxVYLwFZWB7gnq98n2YFoFTQk_XtnQUlQLEeM8dwyUwMQ8O2gQEVQo-ite9bquD-htCw_rbzaFKjU1p8PYuWh-KaH1fU-ZK0Q0tCoTydN8aMbOOdSdLkv-oD_rI9_w</recordid><startdate>20020603</startdate><enddate>20020603</enddate><creator>SONG, BANG HO</creator><creator>BAE, HUI GYEONG</creator><creator>SHIN, PYEONG GYUN</creator><creator>OH, HYEON JU</creator><scope>EVB</scope></search><sort><creationdate>20020603</creationdate><title>Use of plasmid containing luxCDABE and recombinant E. coli SB01 which was transformed by this plasmid</title><author>SONG, BANG HO ; BAE, HUI GYEONG ; SHIN, PYEONG GYUN ; OH, HYEON JU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_KR20020041603A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; kor</language><creationdate>2002</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>SONG, BANG HO</creatorcontrib><creatorcontrib>BAE, HUI GYEONG</creatorcontrib><creatorcontrib>SHIN, PYEONG GYUN</creatorcontrib><creatorcontrib>OH, HYEON JU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SONG, BANG HO</au><au>BAE, HUI GYEONG</au><au>SHIN, PYEONG GYUN</au><au>OH, HYEON JU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Use of plasmid containing luxCDABE and recombinant E. coli SB01 which was transformed by this plasmid</title><date>2002-06-03</date><risdate>2002</risdate><abstract>PURPOSE: A recombinant plasmid containing luxCDABE, E. coli SB01 (KFCC 11176) transformed therewith, and the use thereof are provided, therefore the stability of the recombinant plasmid in E. coli can be increased to use it for the detection of toxicity. CONSTITUTION: The recombinant plasmid pBS1 contains a vector pKLJ12 containing luxCDABE derived from Photorhabdus luminescens. E. coli SB01 KFCC 11176 is produced by transforming E. coli DH5alpha with the recombinant plasmid pBS1. A method for detecting toxicity in a sample solution comprises culturing the transformed microorganism E. coli SB01 KFCC 11176 in the sample solution in a continuous fermenter, and analyzing the luminescence of the sample solution. 본 발명은 발광유전자 luxCDABE를 포함하는 재조합 플라스미드, 이 플라스미드에 의해 형질전환된 대장균 SB01 및 이의 용도에 관한 것으로서, 더욱 상세하게는 육지발광미생물 포토하버드스 루미네슨스()의 발광유전자(luxCDABE)가 삽입된 미니(mini)-F pKLJ12를 포함하는 재조합 플라스미드 pBS1, 이 플라스미드에 의해 형질전환된 대장균 SB01(KFCC 11176)을 제공하며, 이렇게 형질전환된 대장균 SB01이 600 세대(25 일)까지 항생제가 첨가되지 않은 배지상에서 안정된 플라스미드 유지를 보여 플라스미드의 안정성과 발광유전자를 이용해 독성탐지용 연속배양 장치에서의 미생물원으로 이용이 가능하고, 시판중인 발광미생물 킷(kit)의 수입 대체로 인한 경제적인 효과도 나타내는 발광유전자 luxCDABE를 포함하는 재조합 플라스미드, 이 플라스미드에 의해 형질 전환된 대장균 SB01 및 이의 용도에 관한 것이다.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Use of plasmid containing luxCDABE and recombinant E. coli SB01 which was transformed by this plasmid
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