Apparatus and Method for Extracting Genome Using Ultrasound Transducer and Magnetic Bead

Provided is a method for extracting a dielectric. According to an embodiment of the present application, the method for extracting a dielectric comprises the steps of: (a) injecting a solution containing a plurality of magnetic beads (b) into a channel (110); (b) moving a magnetic body (200) to a fi...

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Hauptverfasser: OH MINTACK, LEE SOO HYUN, NAKWON CHOI, LEE BYUNG CHUL, LEE HYUNGBEEN
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LEE SOO HYUN
NAKWON CHOI
LEE BYUNG CHUL
LEE HYUNGBEEN
description Provided is a method for extracting a dielectric. According to an embodiment of the present application, the method for extracting a dielectric comprises the steps of: (a) injecting a solution containing a plurality of magnetic beads (b) into a channel (110); (b) moving a magnetic body (200) to a first position with respect to the channel (110) to aggregate the plurality of magnetic beads (b), and discharging a solution except the plurality of magnetic beads (b) to the outside of the channel (110); (c) injecting a solution containing a lysis buffer and a sample to be analyzed into the channel (110); and (d) moving the magnetic body (200) to a second position with respect to the channel (110), applying ultrasonic energy of a predetermined pattern to the channel (110) to cause the plurality of magnetic beads (b) to collide with a substance included in the sample to be analyzed such that the substance is lysed, and causing a dielectric to be analyzed contained in the substance to be eluted such that the dielectric to be analyzed is adsorbed to the plurality of magnetic beads (b), wherein a distance between the magnetic body (200) and the channel (110) at the first position may be closer than that at the second position. 유전체 추출 방법을 제공한다. 본 출원의 실시예에 따른 유전체 추출 방법은 (a) 복수의 자성 비드(Magnetic Bead)(b)가 포함된 용액이 채널(110)로 투입되는 단계, (b) 자성체(200)가 상기 채널(110)에 대한 제1 위치로 이동하여 상기 복수의 자성 비드(b)가 응집되고, 상기 복수의 자성 비드(b)를 제외한 용액이 상기 채널(110) 외부로 배출되는 단계, (c) 상기 채널(110)에 용해 버퍼(Lysis Buffer) 및 분석 대상 샘플을 포함하는 용액이 투입되는 단계 및 (d) 상기 자성체(200)가 상기 채널(110)에 대한 제2 위치로 이동하고, 상기 채널(110)에 기 결정된 패턴의 초음파 에너지가 인가되어, 상기 복수의 자성 비드(b)가 상기 분석 대상 샘플에 포함된 물질과 충돌하여 상기 물질이 용해(Lysis)되고, 상기 물질에 수용된 분석 대상 유전체가 용출되어 상기 복수의 자성 비드(b)에 상기 분석 대상 유전체가 흡착되는 단계를 포함하며, 상기 제1 위치에서 상기 자성체(200)와 상기 채널(110) 간의 거리는 상기 제2 위치에서보다 가까운 것일 수 있다.
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fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_KR102086637BB1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>KR102086637BB1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_KR102086637BB13</originalsourceid><addsrcrecordid>eNrjZIhwLChILEosKS1WSMxLUfBNLcnIT1FIyy9ScK0oKUpMLsnMS1dwT83Lz01VCC0GcUJzgOLF-aVA1SFFiXnFKaXJqUUQzYnpeaklmckKTqmJKTwMrGmJOcWpvFCam0HZzTXE2UM3tSA_PrW4IDE5Fag43jvI0MDIwMLMzNjcycnQmDhVAEaAOr4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Apparatus and Method for Extracting Genome Using Ultrasound Transducer and Magnetic Bead</title><source>esp@cenet</source><creator>OH MINTACK ; LEE SOO HYUN ; NAKWON CHOI ; LEE BYUNG CHUL ; LEE HYUNGBEEN</creator><creatorcontrib>OH MINTACK ; LEE SOO HYUN ; NAKWON CHOI ; LEE BYUNG CHUL ; LEE HYUNGBEEN</creatorcontrib><description>Provided is a method for extracting a dielectric. According to an embodiment of the present application, the method for extracting a dielectric comprises the steps of: (a) injecting a solution containing a plurality of magnetic beads (b) into a channel (110); (b) moving a magnetic body (200) to a first position with respect to the channel (110) to aggregate the plurality of magnetic beads (b), and discharging a solution except the plurality of magnetic beads (b) to the outside of the channel (110); (c) injecting a solution containing a lysis buffer and a sample to be analyzed into the channel (110); and (d) moving the magnetic body (200) to a second position with respect to the channel (110), applying ultrasonic energy of a predetermined pattern to the channel (110) to cause the plurality of magnetic beads (b) to collide with a substance included in the sample to be analyzed such that the substance is lysed, and causing a dielectric to be analyzed contained in the substance to be eluted such that the dielectric to be analyzed is adsorbed to the plurality of magnetic beads (b), wherein a distance between the magnetic body (200) and the channel (110) at the first position may be closer than that at the second position. 유전체 추출 방법을 제공한다. 본 출원의 실시예에 따른 유전체 추출 방법은 (a) 복수의 자성 비드(Magnetic Bead)(b)가 포함된 용액이 채널(110)로 투입되는 단계, (b) 자성체(200)가 상기 채널(110)에 대한 제1 위치로 이동하여 상기 복수의 자성 비드(b)가 응집되고, 상기 복수의 자성 비드(b)를 제외한 용액이 상기 채널(110) 외부로 배출되는 단계, (c) 상기 채널(110)에 용해 버퍼(Lysis Buffer) 및 분석 대상 샘플을 포함하는 용액이 투입되는 단계 및 (d) 상기 자성체(200)가 상기 채널(110)에 대한 제2 위치로 이동하고, 상기 채널(110)에 기 결정된 패턴의 초음파 에너지가 인가되어, 상기 복수의 자성 비드(b)가 상기 분석 대상 샘플에 포함된 물질과 충돌하여 상기 물질이 용해(Lysis)되고, 상기 물질에 수용된 분석 대상 유전체가 용출되어 상기 복수의 자성 비드(b)에 상기 분석 대상 유전체가 흡착되는 단계를 포함하며, 상기 제1 위치에서 상기 자성체(200)와 상기 채널(110) 간의 거리는 상기 제2 위치에서보다 가까운 것일 수 있다.</description><language>eng ; kor</language><subject>BEER ; BIOCHEMISTRY ; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRANSPORTING ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20200310&amp;DB=EPODOC&amp;CC=KR&amp;NR=102086637B1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25547,76298</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20200310&amp;DB=EPODOC&amp;CC=KR&amp;NR=102086637B1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>OH MINTACK</creatorcontrib><creatorcontrib>LEE SOO HYUN</creatorcontrib><creatorcontrib>NAKWON CHOI</creatorcontrib><creatorcontrib>LEE BYUNG CHUL</creatorcontrib><creatorcontrib>LEE HYUNGBEEN</creatorcontrib><title>Apparatus and Method for Extracting Genome Using Ultrasound Transducer and Magnetic Bead</title><description>Provided is a method for extracting a dielectric. According to an embodiment of the present application, the method for extracting a dielectric comprises the steps of: (a) injecting a solution containing a plurality of magnetic beads (b) into a channel (110); (b) moving a magnetic body (200) to a first position with respect to the channel (110) to aggregate the plurality of magnetic beads (b), and discharging a solution except the plurality of magnetic beads (b) to the outside of the channel (110); (c) injecting a solution containing a lysis buffer and a sample to be analyzed into the channel (110); and (d) moving the magnetic body (200) to a second position with respect to the channel (110), applying ultrasonic energy of a predetermined pattern to the channel (110) to cause the plurality of magnetic beads (b) to collide with a substance included in the sample to be analyzed such that the substance is lysed, and causing a dielectric to be analyzed contained in the substance to be eluted such that the dielectric to be analyzed is adsorbed to the plurality of magnetic beads (b), wherein a distance between the magnetic body (200) and the channel (110) at the first position may be closer than that at the second position. 유전체 추출 방법을 제공한다. 본 출원의 실시예에 따른 유전체 추출 방법은 (a) 복수의 자성 비드(Magnetic Bead)(b)가 포함된 용액이 채널(110)로 투입되는 단계, (b) 자성체(200)가 상기 채널(110)에 대한 제1 위치로 이동하여 상기 복수의 자성 비드(b)가 응집되고, 상기 복수의 자성 비드(b)를 제외한 용액이 상기 채널(110) 외부로 배출되는 단계, (c) 상기 채널(110)에 용해 버퍼(Lysis Buffer) 및 분석 대상 샘플을 포함하는 용액이 투입되는 단계 및 (d) 상기 자성체(200)가 상기 채널(110)에 대한 제2 위치로 이동하고, 상기 채널(110)에 기 결정된 패턴의 초음파 에너지가 인가되어, 상기 복수의 자성 비드(b)가 상기 분석 대상 샘플에 포함된 물질과 충돌하여 상기 물질이 용해(Lysis)되고, 상기 물질에 수용된 분석 대상 유전체가 용출되어 상기 복수의 자성 비드(b)에 상기 분석 대상 유전체가 흡착되는 단계를 포함하며, 상기 제1 위치에서 상기 자성체(200)와 상기 채널(110) 간의 거리는 상기 제2 위치에서보다 가까운 것일 수 있다.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PERFORMING OPERATIONS</subject><subject>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRANSPORTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZIhwLChILEosKS1WSMxLUfBNLcnIT1FIyy9ScK0oKUpMLsnMS1dwT83Lz01VCC0GcUJzgOLF-aVA1SFFiXnFKaXJqUUQzYnpeaklmckKTqmJKTwMrGmJOcWpvFCam0HZzTXE2UM3tSA_PrW4IDE5Fag43jvI0MDIwMLMzNjcycnQmDhVAEaAOr4</recordid><startdate>20200310</startdate><enddate>20200310</enddate><creator>OH MINTACK</creator><creator>LEE SOO HYUN</creator><creator>NAKWON CHOI</creator><creator>LEE BYUNG CHUL</creator><creator>LEE HYUNGBEEN</creator><scope>EVB</scope></search><sort><creationdate>20200310</creationdate><title>Apparatus and Method for Extracting Genome Using Ultrasound Transducer and Magnetic Bead</title><author>OH MINTACK ; LEE SOO HYUN ; NAKWON CHOI ; LEE BYUNG CHUL ; LEE HYUNGBEEN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_KR102086637BB13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; kor</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PERFORMING OPERATIONS</topic><topic>PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRANSPORTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>OH MINTACK</creatorcontrib><creatorcontrib>LEE SOO HYUN</creatorcontrib><creatorcontrib>NAKWON CHOI</creatorcontrib><creatorcontrib>LEE BYUNG CHUL</creatorcontrib><creatorcontrib>LEE HYUNGBEEN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>OH MINTACK</au><au>LEE SOO HYUN</au><au>NAKWON CHOI</au><au>LEE BYUNG CHUL</au><au>LEE HYUNGBEEN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Apparatus and Method for Extracting Genome Using Ultrasound Transducer and Magnetic Bead</title><date>2020-03-10</date><risdate>2020</risdate><abstract>Provided is a method for extracting a dielectric. According to an embodiment of the present application, the method for extracting a dielectric comprises the steps of: (a) injecting a solution containing a plurality of magnetic beads (b) into a channel (110); (b) moving a magnetic body (200) to a first position with respect to the channel (110) to aggregate the plurality of magnetic beads (b), and discharging a solution except the plurality of magnetic beads (b) to the outside of the channel (110); (c) injecting a solution containing a lysis buffer and a sample to be analyzed into the channel (110); and (d) moving the magnetic body (200) to a second position with respect to the channel (110), applying ultrasonic energy of a predetermined pattern to the channel (110) to cause the plurality of magnetic beads (b) to collide with a substance included in the sample to be analyzed such that the substance is lysed, and causing a dielectric to be analyzed contained in the substance to be eluted such that the dielectric to be analyzed is adsorbed to the plurality of magnetic beads (b), wherein a distance between the magnetic body (200) and the channel (110) at the first position may be closer than that at the second position. 유전체 추출 방법을 제공한다. 본 출원의 실시예에 따른 유전체 추출 방법은 (a) 복수의 자성 비드(Magnetic Bead)(b)가 포함된 용액이 채널(110)로 투입되는 단계, (b) 자성체(200)가 상기 채널(110)에 대한 제1 위치로 이동하여 상기 복수의 자성 비드(b)가 응집되고, 상기 복수의 자성 비드(b)를 제외한 용액이 상기 채널(110) 외부로 배출되는 단계, (c) 상기 채널(110)에 용해 버퍼(Lysis Buffer) 및 분석 대상 샘플을 포함하는 용액이 투입되는 단계 및 (d) 상기 자성체(200)가 상기 채널(110)에 대한 제2 위치로 이동하고, 상기 채널(110)에 기 결정된 패턴의 초음파 에너지가 인가되어, 상기 복수의 자성 비드(b)가 상기 분석 대상 샘플에 포함된 물질과 충돌하여 상기 물질이 용해(Lysis)되고, 상기 물질에 수용된 분석 대상 유전체가 용출되어 상기 복수의 자성 비드(b)에 상기 분석 대상 유전체가 흡착되는 단계를 포함하며, 상기 제1 위치에서 상기 자성체(200)와 상기 채널(110) 간의 거리는 상기 제2 위치에서보다 가까운 것일 수 있다.</abstract><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRANSPORTING
VINEGAR
WINE
title Apparatus and Method for Extracting Genome Using Ultrasound Transducer and Magnetic Bead
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T15%3A56%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=OH%20MINTACK&rft.date=2020-03-10&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EKR102086637BB1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true