SEPARATION AND PURIFICATION OF 3-OXO-5BETA-STEROID-DELTA4-DEHYDROGENASE
PURPOSE:To obtain high purity 3-oxo-5beta-steroid-DELTA -dehydrogenase (3-OSD) in separated and purified state on an industrial scale, by adding a specific gel to a solution containing 3-OSD, thereby allowing the gel to adsorb 3-OSD. CONSTITUTION:Polyvinyl gel or agarose gel having bonded butyl grou...
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| creator | USHIZAWA KOJI HIBINO MITSUGI MAKI AKEMICHI HIRATA MITSUYOSHI |
| description | PURPOSE:To obtain high purity 3-oxo-5beta-steroid-DELTA -dehydrogenase (3-OSD) in separated and purified state on an industrial scale, by adding a specific gel to a solution containing 3-OSD, thereby allowing the gel to adsorb 3-OSD. CONSTITUTION:Polyvinyl gel or agarose gel having bonded butyl group is suspended in a salt buffer solution of about 7pH at a concentration of 10-30wt% and the suspension is filled in a column. A 3-OSD solution produced by dissolving 3-OSD in a buffer solution having an electrical conductivity of 50-100mmho/cm and a definite pH is filled in the column to effect the adsorption of 3-OSD to the gel. A buffer solution same as the above buffer solution is passed through the column at an amount corresponding to 2-3 times the volume of the column to elute impurities. After the above treatment, a buffer solution of 7pH having low salt concentration is passed through the column to elute the enzyme. High purity 3-OSD produced by this process has a purification ratio of as high as 21-35 based on the crude raw material. |
| format | Patent |
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CONSTITUTION:Polyvinyl gel or agarose gel having bonded butyl group is suspended in a salt buffer solution of about 7pH at a concentration of 10-30wt% and the suspension is filled in a column. A 3-OSD solution produced by dissolving 3-OSD in a buffer solution having an electrical conductivity of 50-100mmho/cm and a definite pH is filled in the column to effect the adsorption of 3-OSD to the gel. A buffer solution same as the above buffer solution is passed through the column at an amount corresponding to 2-3 times the volume of the column to elute impurities. After the above treatment, a buffer solution of 7pH having low salt concentration is passed through the column to elute the enzyme. High purity 3-OSD produced by this process has a purification ratio of as high as 21-35 based on the crude raw material.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1987</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19871116&DB=EPODOC&CC=JP&NR=S62262991A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19871116&DB=EPODOC&CC=JP&NR=S62262991A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>USHIZAWA KOJI</creatorcontrib><creatorcontrib>HIBINO MITSUGI</creatorcontrib><creatorcontrib>MAKI AKEMICHI</creatorcontrib><creatorcontrib>HIRATA MITSUYOSHI</creatorcontrib><title>SEPARATION AND PURIFICATION OF 3-OXO-5BETA-STEROID-DELTA4-DEHYDROGENASE</title><description>PURPOSE:To obtain high purity 3-oxo-5beta-steroid-DELTA -dehydrogenase (3-OSD) in separated and purified state on an industrial scale, by adding a specific gel to a solution containing 3-OSD, thereby allowing the gel to adsorb 3-OSD. CONSTITUTION:Polyvinyl gel or agarose gel having bonded butyl group is suspended in a salt buffer solution of about 7pH at a concentration of 10-30wt% and the suspension is filled in a column. A 3-OSD solution produced by dissolving 3-OSD in a buffer solution having an electrical conductivity of 50-100mmho/cm and a definite pH is filled in the column to effect the adsorption of 3-OSD to the gel. A buffer solution same as the above buffer solution is passed through the column at an amount corresponding to 2-3 times the volume of the column to elute impurities. After the above treatment, a buffer solution of 7pH having low salt concentration is passed through the column to elute the enzyme. 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CONSTITUTION:Polyvinyl gel or agarose gel having bonded butyl group is suspended in a salt buffer solution of about 7pH at a concentration of 10-30wt% and the suspension is filled in a column. A 3-OSD solution produced by dissolving 3-OSD in a buffer solution having an electrical conductivity of 50-100mmho/cm and a definite pH is filled in the column to effect the adsorption of 3-OSD to the gel. A buffer solution same as the above buffer solution is passed through the column at an amount corresponding to 2-3 times the volume of the column to elute impurities. After the above treatment, a buffer solution of 7pH having low salt concentration is passed through the column to elute the enzyme. High purity 3-OSD produced by this process has a purification ratio of as high as 21-35 based on the crude raw material.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | SEPARATION AND PURIFICATION OF 3-OXO-5BETA-STEROID-DELTA4-DEHYDROGENASE |
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