MICRODETERMINATION OF COMPOUND HAVING PYRIMIDINE RING
PURPOSE:To enable microdetermination of a pyrimidine compd. which has a pyrimidine ring in the chemical structure and in which at least one of the nitrogen raw material in the ring is not substd. by subjecting the product obtd. by treating a sample with 4- bromomethyl-7-methoxy cumarine in the prese...
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| creator | YOSHIDA SHIGERU IWAMOTO MAKI CHIYOU TOSHIO HIROSE SHINGO |
| description | PURPOSE:To enable microdetermination of a pyrimidine compd. which has a pyrimidine ring in the chemical structure and in which at least one of the nitrogen raw material in the ring is not substd. by subjecting the product obtd. by treating a sample with 4- bromomethyl-7-methoxy cumarine in the presence of crown ethers to fluorescent high- speed liquid chromatography. CONSTITUTION:The compd. to be measured is a pyrimidine compd. which has a pyrimidine ring in the chemical structure and in which at least one of the nitrogen raw material in the ring has no substituent. Such compd. is exemplified by the base, nucleotide and nucleocide of a pyrimidine nucleic acid and the embodiment thereof is enumerated by uracil, thymine, cytosine, etc. The quantitative determination thereof is accomplished by treating the sample with 4-bromomethyl-7-methoxy cumarine in the presence of crown ethers. The pyrimidine compd. in the sample to be measured reacts with the 4- bromomethyl-7-methoxy cumarine as a result of such treatment and forms a fluorescence-emitting compd. The fluorescence-emitting compd. is the compd. in which the methyl group is bound to the nitrogen atom site having no substituent in the pyrimidine ring. Such fluorescence-emitting compd. is subjected to fluorescent high-speed liquid chromatography and is determined quantitatively by using a fluorescence detector. |
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| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_JPS6036954A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>JPS6036954A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_JPS6036954A3</originalsourceid><addsrcrecordid>eNrjZDD19XQO8ndxDXEN8vX0cwzx9PdT8HdTcPb3DfAP9XNR8HAM8_RzVwiIDPL09XTx9HNVCALyeRhY0xJzilN5oTQ3g4Kba4izh25qQX58anFBYnJqXmpJvFdAsJmBsZmlqYmjMRFKAM4eJ-I</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>MICRODETERMINATION OF COMPOUND HAVING PYRIMIDINE RING</title><source>esp@cenet</source><creator>YOSHIDA SHIGERU ; IWAMOTO MAKI ; CHIYOU TOSHIO ; HIROSE SHINGO</creator><creatorcontrib>YOSHIDA SHIGERU ; IWAMOTO MAKI ; CHIYOU TOSHIO ; HIROSE SHINGO</creatorcontrib><description>PURPOSE:To enable microdetermination of a pyrimidine compd. which has a pyrimidine ring in the chemical structure and in which at least one of the nitrogen raw material in the ring is not substd. by subjecting the product obtd. by treating a sample with 4- bromomethyl-7-methoxy cumarine in the presence of crown ethers to fluorescent high- speed liquid chromatography. CONSTITUTION:The compd. to be measured is a pyrimidine compd. which has a pyrimidine ring in the chemical structure and in which at least one of the nitrogen raw material in the ring has no substituent. Such compd. is exemplified by the base, nucleotide and nucleocide of a pyrimidine nucleic acid and the embodiment thereof is enumerated by uracil, thymine, cytosine, etc. The quantitative determination thereof is accomplished by treating the sample with 4-bromomethyl-7-methoxy cumarine in the presence of crown ethers. The pyrimidine compd. in the sample to be measured reacts with the 4- bromomethyl-7-methoxy cumarine as a result of such treatment and forms a fluorescence-emitting compd. The fluorescence-emitting compd. is the compd. in which the methyl group is bound to the nitrogen atom site having no substituent in the pyrimidine ring. Such fluorescence-emitting compd. is subjected to fluorescent high-speed liquid chromatography and is determined quantitatively by using a fluorescence detector.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>1985</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19850226&DB=EPODOC&CC=JP&NR=S6036954A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76294</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19850226&DB=EPODOC&CC=JP&NR=S6036954A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YOSHIDA SHIGERU</creatorcontrib><creatorcontrib>IWAMOTO MAKI</creatorcontrib><creatorcontrib>CHIYOU TOSHIO</creatorcontrib><creatorcontrib>HIROSE SHINGO</creatorcontrib><title>MICRODETERMINATION OF COMPOUND HAVING PYRIMIDINE RING</title><description>PURPOSE:To enable microdetermination of a pyrimidine compd. which has a pyrimidine ring in the chemical structure and in which at least one of the nitrogen raw material in the ring is not substd. by subjecting the product obtd. by treating a sample with 4- bromomethyl-7-methoxy cumarine in the presence of crown ethers to fluorescent high- speed liquid chromatography. CONSTITUTION:The compd. to be measured is a pyrimidine compd. which has a pyrimidine ring in the chemical structure and in which at least one of the nitrogen raw material in the ring has no substituent. Such compd. is exemplified by the base, nucleotide and nucleocide of a pyrimidine nucleic acid and the embodiment thereof is enumerated by uracil, thymine, cytosine, etc. The quantitative determination thereof is accomplished by treating the sample with 4-bromomethyl-7-methoxy cumarine in the presence of crown ethers. The pyrimidine compd. in the sample to be measured reacts with the 4- bromomethyl-7-methoxy cumarine as a result of such treatment and forms a fluorescence-emitting compd. The fluorescence-emitting compd. is the compd. in which the methyl group is bound to the nitrogen atom site having no substituent in the pyrimidine ring. Such fluorescence-emitting compd. is subjected to fluorescent high-speed liquid chromatography and is determined quantitatively by using a fluorescence detector.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1985</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDD19XQO8ndxDXEN8vX0cwzx9PdT8HdTcPb3DfAP9XNR8HAM8_RzVwiIDPL09XTx9HNVCALyeRhY0xJzilN5oTQ3g4Kba4izh25qQX58anFBYnJqXmpJvFdAsJmBsZmlqYmjMRFKAM4eJ-I</recordid><startdate>19850226</startdate><enddate>19850226</enddate><creator>YOSHIDA SHIGERU</creator><creator>IWAMOTO MAKI</creator><creator>CHIYOU TOSHIO</creator><creator>HIROSE SHINGO</creator><scope>EVB</scope></search><sort><creationdate>19850226</creationdate><title>MICRODETERMINATION OF COMPOUND HAVING PYRIMIDINE RING</title><author>YOSHIDA SHIGERU ; IWAMOTO MAKI ; CHIYOU TOSHIO ; HIROSE SHINGO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_JPS6036954A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>1985</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YOSHIDA SHIGERU</creatorcontrib><creatorcontrib>IWAMOTO MAKI</creatorcontrib><creatorcontrib>CHIYOU TOSHIO</creatorcontrib><creatorcontrib>HIROSE SHINGO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YOSHIDA SHIGERU</au><au>IWAMOTO MAKI</au><au>CHIYOU TOSHIO</au><au>HIROSE SHINGO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>MICRODETERMINATION OF COMPOUND HAVING PYRIMIDINE RING</title><date>1985-02-26</date><risdate>1985</risdate><abstract>PURPOSE:To enable microdetermination of a pyrimidine compd. which has a pyrimidine ring in the chemical structure and in which at least one of the nitrogen raw material in the ring is not substd. by subjecting the product obtd. by treating a sample with 4- bromomethyl-7-methoxy cumarine in the presence of crown ethers to fluorescent high- speed liquid chromatography. CONSTITUTION:The compd. to be measured is a pyrimidine compd. which has a pyrimidine ring in the chemical structure and in which at least one of the nitrogen raw material in the ring has no substituent. Such compd. is exemplified by the base, nucleotide and nucleocide of a pyrimidine nucleic acid and the embodiment thereof is enumerated by uracil, thymine, cytosine, etc. The quantitative determination thereof is accomplished by treating the sample with 4-bromomethyl-7-methoxy cumarine in the presence of crown ethers. The pyrimidine compd. in the sample to be measured reacts with the 4- bromomethyl-7-methoxy cumarine as a result of such treatment and forms a fluorescence-emitting compd. The fluorescence-emitting compd. is the compd. in which the methyl group is bound to the nitrogen atom site having no substituent in the pyrimidine ring. Such fluorescence-emitting compd. is subjected to fluorescent high-speed liquid chromatography and is determined quantitatively by using a fluorescence detector.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | MICRODETERMINATION OF COMPOUND HAVING PYRIMIDINE RING |
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