PREPARATION OF HEAT-RESISTANT ENZYME

PURPOSE:To prepare a heat-resistant enzyme having high specific activity, in high efficiency, by completely sterilizing a transformed strain which can be obtained in large amount by cultivation, and reacting the product with a bacteriolytic enzyme. CONSTITUTION:A transformed bacterial strain such as...

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BETSUPU TERUHIKO
description PURPOSE:To prepare a heat-resistant enzyme having high specific activity, in high efficiency, by completely sterilizing a transformed strain which can be obtained in large amount by cultivation, and reacting the product with a bacteriolytic enzyme. CONSTITUTION:A transformed bacterial strain such as Thermus flavus AT62 strain is obtained by the insertion of a plasmid integrated with the gene of heat-resistant enzyme. Escherichia coli C600 strain is transformed to Escherichia coli F-74 strain (FERM-P No.6698) by plasmid PBR322 containing the gene of the heat-resistant L-alanine dehydrogenase of the above transformed bacterial strain. Thermus flavus AT62. The obtained F-74 strain is cultured, and the cultured cells are collected and suspended to obtain a cell suspension. The suspension or the above cultured liquid is heated to effect the complete sterilization of the bacterial cells, and the obtained killed cells are collected and suspended. A bacteriolytic enzyme or the enzyme and a surface active agent are added to the above suspension to dissolve the heat-resistant enzyme from the bacterial cell to the suspension.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title PREPARATION OF HEAT-RESISTANT ENZYME
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