CULTURE OF MICROBIAL CELL
PROBLEM TO BE SOLVED: To provide a method for efficiently culturing Pseudomonas bacteria having the ability to stably degrade TCE. SOLUTION: This method comprises culturing Pseudomonas bacteria having the ability to degrade trichloroethylene (TCE); specifically, the method is as follows: Pseudomonas...
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| creator | OSAWA TAKEHIKO KITAGAWA MASAYASU HIROSE AKIRA |
| description | PROBLEM TO BE SOLVED: To provide a method for efficiently culturing Pseudomonas bacteria having the ability to stably degrade TCE. SOLUTION: This method comprises culturing Pseudomonas bacteria having the ability to degrade trichloroethylene (TCE); specifically, the method is as follows: Pseudomonas bacteria is subjected to preculture at 10-40 deg.C using a culture fluid at pH 5-9 with an initial toluene concentration of 100-400 mg/L containing at least a phosphorus source, nitrogen source, calcium, magnesium and iron; wherein it is preferable that the preculture is carried out by repeating batch cultures twice to seven times. |
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SOLUTION: This method comprises culturing Pseudomonas bacteria having the ability to degrade trichloroethylene (TCE); specifically, the method is as follows: Pseudomonas bacteria is subjected to preculture at 10-40 deg.C using a culture fluid at pH 5-9 with an initial toluene concentration of 100-400 mg/L containing at least a phosphorus source, nitrogen source, calcium, magnesium and iron; wherein it is preferable that the preculture is carried out by repeating batch cultures twice to seven times.</description><edition>6</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; DISPOSAL OF SOLID WASTE ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PERFORMING OPERATIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; RECLAMATION OF CONTAMINATED SOIL ; RECLAMATION OF CONTAMINED SOIL SOIL ; SPIRITS ; TRANSPORTING ; TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE ; VINEGAR ; WINE</subject><creationdate>1999</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19990223&DB=EPODOC&CC=JP&NR=H1146758A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25569,76552</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19990223&DB=EPODOC&CC=JP&NR=H1146758A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>OSAWA TAKEHIKO</creatorcontrib><creatorcontrib>KITAGAWA MASAYASU</creatorcontrib><creatorcontrib>HIROSE AKIRA</creatorcontrib><title>CULTURE OF MICROBIAL CELL</title><description>PROBLEM TO BE SOLVED: To provide a method for efficiently culturing Pseudomonas bacteria having the ability to stably degrade TCE. 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SOLUTION: This method comprises culturing Pseudomonas bacteria having the ability to degrade trichloroethylene (TCE); specifically, the method is as follows: Pseudomonas bacteria is subjected to preculture at 10-40 deg.C using a culture fluid at pH 5-9 with an initial toluene concentration of 100-400 mg/L containing at least a phosphorus source, nitrogen source, calcium, magnesium and iron; wherein it is preferable that the preculture is carried out by repeating batch cultures twice to seven times.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DISPOSAL OF SOLID WASTE ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECLAMATION OF CONTAMINATED SOIL RECLAMATION OF CONTAMINED SOIL SOIL SPIRITS TRANSPORTING TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE VINEGAR WINE |
| title | CULTURE OF MICROBIAL CELL |
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