METHOD FOR WASHING LIQUID CHROMATOGRAPHY CARRIER AND ITS WASHING LIQUID

PROBLEM TO BE SOLVED: To wash a substance being attracted to a chromatography carrier quickly by containing or injecting a washing liquid containing oxygen in a mobile phase in a liquid chromatography that is being operated at least at a specific pressure. SOLUTION: Oxygen contained in a washing liquid is one corresponding to a constituent being attracted in a carrier and is selected according to the type of a sample to be inspected. The concentration of enzyme is determined so that a high enzyme activity can be obtained when the carrier touches the enzyme by considering condition such as pH in use, temperature, sample type, its concentration, and time for using the carrier. The concentration of a buffer agent in the washing liquid is determined to a concentration for maintaining a constant pH and for obtaining a high enzyme activity. Stabilization agents and activation agents that can be used generally as those of enzyme are selected. Jamming caused by the adsorption of protein or the like to the carrier can be decomposed and eliminated by a washing method and a washing liquid. The retention capacity of an analysis target in a sample and the pressure increase in the carrier being generated when a sample containing a organism constituent is directly injected cannot occur easily, thus using the carrier for a long time.