DETECTION OF ENZYME, COMPOSITION THEREFOR AND TEST SPECIMEN THEREFOR
PROBLEM TO BE SOLVED: To assay an enzymatic activity in a short time by bringing a fluid test specimen into contact with such a protease as to deactivate the aimed enzyme emitting a detectable response in a specified time but not to deactivate a reagent system providing the detectable response. SOLU...
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| creator | SCHAEPER ROBERT J PUGIA MICHAEL J |
| description | PROBLEM TO BE SOLVED: To assay an enzymatic activity in a short time by bringing a fluid test specimen into contact with such a protease as to deactivate the aimed enzyme emitting a detectable response in a specified time but not to deactivate a reagent system providing the detectable response. SOLUTION: In this assay of enzymatic activity in a fluid test specimen that the aimed enzyme catalyzes the reaction in the fluid test specimen providing a detectable response reflecting the concentration of the enzyme or enzyme substrate in the specimen, a quantitated fluid test specimen is added to such a quantitated protease as to deactivate the aimed enzyme to emit a detectable response so that the reaction providing a color reaction ceases after a specified time determined by the concentration of the protease in a reagent system but not to deactivate the reagent system providing the detectable response, to carry out a reaction and detect the detectable response so as to reach the endpoint of the enzyme catalytic reaction in a short time, thus quickly assaying the enzymatic activity in the fluid test specimen such as body fluid. |
| format | Patent |
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SOLUTION: In this assay of enzymatic activity in a fluid test specimen that the aimed enzyme catalyzes the reaction in the fluid test specimen providing a detectable response reflecting the concentration of the enzyme or enzyme substrate in the specimen, a quantitated fluid test specimen is added to such a quantitated protease as to deactivate the aimed enzyme to emit a detectable response so that the reaction providing a color reaction ceases after a specified time determined by the concentration of the protease in a reagent system but not to deactivate the reagent system providing the detectable response, to carry out a reaction and detect the detectable response so as to reach the endpoint of the enzyme catalytic reaction in a short time, thus quickly assaying the enzymatic activity in the fluid test specimen such as body fluid.</description><edition>6</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1998</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19980217&DB=EPODOC&CC=JP&NR=H1042895A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19980217&DB=EPODOC&CC=JP&NR=H1042895A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SCHAEPER ROBERT J</creatorcontrib><creatorcontrib>PUGIA MICHAEL J</creatorcontrib><title>DETECTION OF ENZYME, COMPOSITION THEREFOR AND TEST SPECIMEN THEREFOR</title><description>PROBLEM TO BE SOLVED: To assay an enzymatic activity in a short time by bringing a fluid test specimen into contact with such a protease as to deactivate the aimed enzyme emitting a detectable response in a specified time but not to deactivate a reagent system providing the detectable response. 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SOLUTION: In this assay of enzymatic activity in a fluid test specimen that the aimed enzyme catalyzes the reaction in the fluid test specimen providing a detectable response reflecting the concentration of the enzyme or enzyme substrate in the specimen, a quantitated fluid test specimen is added to such a quantitated protease as to deactivate the aimed enzyme to emit a detectable response so that the reaction providing a color reaction ceases after a specified time determined by the concentration of the protease in a reagent system but not to deactivate the reagent system providing the detectable response, to carry out a reaction and detect the detectable response so as to reach the endpoint of the enzyme catalytic reaction in a short time, thus quickly assaying the enzymatic activity in the fluid test specimen such as body fluid.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | DETECTION OF ENZYME, COMPOSITION THEREFOR AND TEST SPECIMEN THEREFOR |
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