DETECTION OF ENZYME, COMPOSITION THEREFOR AND TEST SPECIMEN THEREFOR

PROBLEM TO BE SOLVED: To assay an enzymatic activity in a short time by bringing a fluid test specimen into contact with such a protease as to deactivate the aimed enzyme emitting a detectable response in a specified time but not to deactivate a reagent system providing the detectable response. SOLUTION: In this assay of enzymatic activity in a fluid test specimen that the aimed enzyme catalyzes the reaction in the fluid test specimen providing a detectable response reflecting the concentration of the enzyme or enzyme substrate in the specimen, a quantitated fluid test specimen is added to such a quantitated protease as to deactivate the aimed enzyme to emit a detectable response so that the reaction providing a color reaction ceases after a specified time determined by the concentration of the protease in a reagent system but not to deactivate the reagent system providing the detectable response, to carry out a reaction and detect the detectable response so as to reach the endpoint of the enzyme catalytic reaction in a short time, thus quickly assaying the enzymatic activity in the fluid test specimen such as body fluid.