MULTILAMELLAR LIPOSOME FOR EMBEDDING PLASMID THEREIN

PROBLEM TO BE SOLVED: To obtain the subject liposome, having a high capturing efficiency of a plasmid, a high expression efficiency of a product with a cell and low toxicity to the cell and useful for gene therapy, etc., by using a specific amount of a specified lipid, etc., having a positive charge...

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Bibliographische Detailangaben
Hauptverfasser:	YAGI KUNIO, KITO MARIKO, OISHI SHIGEKO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS SUGARS THEIR RELEVANT APPARATUS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To obtain the subject liposome, having a high capturing efficiency of a plasmid, a high expression efficiency of a product with a cell and low toxicity to the cell and useful for gene therapy, etc., by using a specific amount of a specified lipid, etc., having a positive charge as a constituent ingredient. SOLUTION: This multilamellar liposome for embedding a plasmid therein comprises N-(α-trimethylammonioacetyl)-didodecyl-D-glutamate chloride(TMAG), dioctanoylphosphatidylcholine(DOPC) and dioleoylphosphatidylethanolamine(DOPE), which are positively charged lipids as constituent lipids. The multilamellar liposome is obtained by preparing a lipid thin membrane at 2:5:3 the molar ratio of the TMAG:DOPC:DOPE, adding a phosphoric acid buffer physiological saline solution containing the plasmid thereto and carrying out the vortex stirring. The resultant liposome has a high capturing efficiency of the plasmid, an ultrahigh expression efficiency of a product with a cell and ultralow toxicity to the cell and suitable for gene therapy, etc.