HEAT RESISTANT METHIONINE AMINOPEPTITASE AND ITS GENE
PURPOSE:To obtain the subject new heat resistant enzyme having a specific amino acid sequence and heat resistance, having a remained methionine group corresponding to an initiation codon at the amino terminus of a protein produced by gene recombinant technique and useful in the field of protein engi...
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| creator | TSUNASAWA SUSUMU KATOU IKUNOSHIN IZU YUKIKO MITSUNAGA KENICHI NAKURA SATOMI |
| description | PURPOSE:To obtain the subject new heat resistant enzyme having a specific amino acid sequence and heat resistance, having a remained methionine group corresponding to an initiation codon at the amino terminus of a protein produced by gene recombinant technique and useful in the field of protein engineering, e.g. treatment of protein. CONSTITUTION:This is a new heat resistant methionine aminopeptitase useful in the field of gene technology, etc., having an amino acid sequence containing an amino acid sequence of the formula, preserving about >=95% of activity after the treatment at 75 deg.C for 60min, having a remained methionine group corresponding to an initiation codon at the amino terminus of a protein produced by gene recombinant technique, capable of giving a matured-type protein by selectively removing the methionine group at the amino terminus by treating its precursor protein. This enzyme is obtained by preparing a library from genomic DNAs isolated from Pyrococcus furiosus DSM 3638 by an ordinary method, selecting a gene by screening the library, combining the gene with a vector to prepare a recombinant plasmid, transducing the recombinant molecule into a host and making the gene to express. |
| format | Patent |
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CONSTITUTION:This is a new heat resistant methionine aminopeptitase useful in the field of gene technology, etc., having an amino acid sequence containing an amino acid sequence of the formula, preserving about >=95% of activity after the treatment at 75 deg.C for 60min, having a remained methionine group corresponding to an initiation codon at the amino terminus of a protein produced by gene recombinant technique, capable of giving a matured-type protein by selectively removing the methionine group at the amino terminus by treating its precursor protein. This enzyme is obtained by preparing a library from genomic DNAs isolated from Pyrococcus furiosus DSM 3638 by an ordinary method, selecting a gene by screening the library, combining the gene with a vector to prepare a recombinant plasmid, transducing the recombinant molecule into a host and making the gene to express.</description><edition>6</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1996</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19960116&DB=EPODOC&CC=JP&NR=H089979A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19960116&DB=EPODOC&CC=JP&NR=H089979A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TSUNASAWA SUSUMU</creatorcontrib><creatorcontrib>KATOU IKUNOSHIN</creatorcontrib><creatorcontrib>IZU YUKIKO</creatorcontrib><creatorcontrib>MITSUNAGA KENICHI</creatorcontrib><creatorcontrib>NAKURA SATOMI</creatorcontrib><title>HEAT RESISTANT METHIONINE AMINOPEPTITASE AND ITS GENE</title><description>PURPOSE:To obtain the subject new heat resistant enzyme having a specific amino acid sequence and heat resistance, having a remained methionine group corresponding to an initiation codon at the amino terminus of a protein produced by gene recombinant technique and useful in the field of protein engineering, e.g. treatment of protein. 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CONSTITUTION:This is a new heat resistant methionine aminopeptitase useful in the field of gene technology, etc., having an amino acid sequence containing an amino acid sequence of the formula, preserving about >=95% of activity after the treatment at 75 deg.C for 60min, having a remained methionine group corresponding to an initiation codon at the amino terminus of a protein produced by gene recombinant technique, capable of giving a matured-type protein by selectively removing the methionine group at the amino terminus by treating its precursor protein. This enzyme is obtained by preparing a library from genomic DNAs isolated from Pyrococcus furiosus DSM 3638 by an ordinary method, selecting a gene by screening the library, combining the gene with a vector to prepare a recombinant plasmid, transducing the recombinant molecule into a host and making the gene to express.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | HEAT RESISTANT METHIONINE AMINOPEPTITASE AND ITS GENE |
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