METHOD FOR MEASURING ACTIVITY OF AMYLASE WITH FLOW INJECTION

PURPOSE: To accurately measure the activity of the amylase in a short time by treating starch with amylase, treating the maltose-produced solution with glucoamylase by a flow injection analysis method, oxidizing the treated product, and subsequently measuring the amount of the consumed oxygen with electrodes. CONSTITUTION: The method for measuring the activity of the amylase by a flow injection analysis method comprises charging the wheat flour of specimen into a test tube 21 with a spoon 20, adding an acetic acid buffer solution 25 into the test tube 21, stirring the mixture with a mixer 24, filtering the treated mixture, charging the filtrate into a syringe 23, adding the charged filtrate into an amylase solution through a filter 22 disposed at the tip of the syringe, adding the mixture into a reaction container 1 in which the acetic acid buffer solution of wheat flour is charged, thus hydrolyzing the starch at 30 deg.C for 10min, injecting the hydrolysis solution with a specimen injector 3 into an acetic acid buffer solution flow flowing through a Teflon tube 5 with a constant delivery pump 6 in an injector 9 for measuring the amount of the produced maltose by the flow injection analysis method, allowing the buffer solution flow to pass through a column 10 charged with immobilizing enzymes comprising glucoamylase and glucose oxidase, oxidizing the produced glucose, and subsequently measuring the amount of the consumed oxygen with a dissolved oxygen electrode 11 in a flow cell to measure the activity of the amylase.